CHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt

Colistin resistance (Col-R) has been rising worldwide with high rate of morbidity and mortality. The emergence of mobile colistin resistance (mcr) harboring microorganisms become of a health concern. Hence, screening for fecal carriage of Col-R Enterobacteriaceae could aid in the prevention and c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Noha M. Mahmoud, Samah S. El-Kazzaz
Formato:	article
Lenguaje:	EN
Publicado:	Ain Shams University 2021
Materias:	colistin resistance fecal carriage mobile colistin resistance genes enterobacteriaceae mcr-1 gene Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8708fb79043d433abc6874f44de41a7e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8708fb79043d433abc6874f44de41a7e
record_format	dspace
spelling	oai:doaj.org-article:8708fb79043d433abc6874f44de41a7e2021-11-15T10:49:16ZCHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt10.21608/nrmj.2021.2048012537-02862537-0294https://doaj.org/article/8708fb79043d433abc6874f44de41a7e2021-11-01T00:00:00Zhttps://nrmj.journals.ekb.eg/article_204801_8e4af2360d1cfa1622c802b7764adadd.pdfhttps://doaj.org/toc/2537-0286https://doaj.org/toc/2537-0294Colistin resistance (Col-R) has been rising worldwide with high rate of morbidity and mortality. The emergence of mobile colistin resistance (mcr) harboring microorganisms become of a health concern. Hence, screening for fecal carriage of Col-R Enterobacteriaceae could aid in the prevention and control efforts of Col-R. This study aimed to screen for Col-R Enterobacteriaceae in the stool specimens of the hospitalized patients; explore for colistin minimal inhibitory concentrations (MIC) and the genetic determinants of these isolates, and to predict the risk factors among the studied patients' groups. Stool specimens from 290 hospitalized adult patients were screened for the presence of Col-R bacterial isolates using CHROMagar COL-APSE medium. Colistin MIC was estimated for Col-R Enterobacteriaceae using the broth microdilution (BMD) assay. Bacterial isolates were screened through the Polymerase chain reaction (PCR) for the existence of mcr-1 and mcr-2 genes. The fecal carriage of Col-R among the studied patients was 16.8 %. About 72 Col-R bacterial isolates were recovered. Col-R Enterobacteriaceae were predominant and were detected in 89.7 % of the bacterial isolates. Using the BMD, Col-R was confirmed and most of the isolates showed low resistance MIC titer (4 μg/ ml; 55.7 %). In addition, mcr-1 gene was the most frequent Col-R gene detected (69.2 %), while mcr-2 gene was less prevalent (11.5 %). The current study reported high prevalence of the Col-R and mcr-1 gene harbored by the fecal flora; with the risk to be easily transmitted inside the hospitals and within the different communities. This highlights the need for active surveillance in addition to the infection control programsNoha M. MahmoudSamah S. El-KazzazAin Shams Universityarticlecolistin resistancefecal carriagemobile colistin resistance genesenterobacteriaceaemcr-1 geneMicrobiologyQR1-502ENNovel Research in Microbiology Journal, Vol 5, Iss 6, Pp 1415-1430 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	colistin resistance fecal carriage mobile colistin resistance genes enterobacteriaceae mcr-1 gene Microbiology QR1-502
spellingShingle	colistin resistance fecal carriage mobile colistin resistance genes enterobacteriaceae mcr-1 gene Microbiology QR1-502 Noha M. Mahmoud Samah S. El-Kazzaz CHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt
description	Colistin resistance (Col-R) has been rising worldwide with high rate of morbidity and mortality. The emergence of mobile colistin resistance (mcr) harboring microorganisms become of a health concern. Hence, screening for fecal carriage of Col-R Enterobacteriaceae could aid in the prevention and control efforts of Col-R. This study aimed to screen for Col-R Enterobacteriaceae in the stool specimens of the hospitalized patients; explore for colistin minimal inhibitory concentrations (MIC) and the genetic determinants of these isolates, and to predict the risk factors among the studied patients' groups. Stool specimens from 290 hospitalized adult patients were screened for the presence of Col-R bacterial isolates using CHROMagar COL-APSE medium. Colistin MIC was estimated for Col-R Enterobacteriaceae using the broth microdilution (BMD) assay. Bacterial isolates were screened through the Polymerase chain reaction (PCR) for the existence of mcr-1 and mcr-2 genes. The fecal carriage of Col-R among the studied patients was 16.8 %. About 72 Col-R bacterial isolates were recovered. Col-R Enterobacteriaceae were predominant and were detected in 89.7 % of the bacterial isolates. Using the BMD, Col-R was confirmed and most of the isolates showed low resistance MIC titer (4 μg/ ml; 55.7 %). In addition, mcr-1 gene was the most frequent Col-R gene detected (69.2 %), while mcr-2 gene was less prevalent (11.5 %). The current study reported high prevalence of the Col-R and mcr-1 gene harbored by the fecal flora; with the risk to be easily transmitted inside the hospitals and within the different communities. This highlights the need for active surveillance in addition to the infection control programs
format	article
author	Noha M. Mahmoud Samah S. El-Kazzaz
author_facet	Noha M. Mahmoud Samah S. El-Kazzaz
author_sort	Noha M. Mahmoud
title	CHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt
title_short	CHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt
title_full	CHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt
title_fullStr	CHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt
title_full_unstemmed	CHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt
title_sort	chromagar col-apse medium as a screening method for fecal carriage of colistin resistant enterobacteriaceae among patients in mansoura university hospitals, egypt
publisher	Ain Shams University
publishDate	2021
url	https://doaj.org/article/8708fb79043d433abc6874f44de41a7e
work_keys_str_mv	AT nohammahmoud chromagarcolapsemediumasascreeningmethodforfecalcarriageofcolistinresistantenterobacteriaceaeamongpatientsinmansourauniversityhospitalsegypt AT samahselkazzaz chromagarcolapsemediumasascreeningmethodforfecalcarriageofcolistinresistantenterobacteriaceaeamongpatientsinmansourauniversityhospitalsegypt
_version_	1718428444453240832

CHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt

Ejemplares similares