Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex

Abstract Albumin re-uptake is a receptor-mediated pathway located in renal proximal tubuli. There is increasing evidence of glomerular protein handling by podocytes, but little is known about the mechanism behind this process. In this study, we found that human podocytes in vitro are committed to in...

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Autores principales:	Lisa Gianesello, Giovanna Priante, Monica Ceol, Claudia M. Radu, Moin A. Saleem, Paolo Simioni, Liliana Terrin, Franca Anglani, Dorella Del Prete
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/870c4846e16141b892ddc0d384672106
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spelling	oai:doaj.org-article:870c4846e16141b892ddc0d3846721062021-12-02T15:05:53ZAlbumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex10.1038/s41598-017-13789-z2045-2322https://doaj.org/article/870c4846e16141b892ddc0d3846721062017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-13789-zhttps://doaj.org/toc/2045-2322Abstract Albumin re-uptake is a receptor-mediated pathway located in renal proximal tubuli. There is increasing evidence of glomerular protein handling by podocytes, but little is known about the mechanism behind this process. In this study, we found that human podocytes in vitro are committed to internalizing albumin through a receptor-mediated mechanism even after exposure to low doses of albumin. We show that these cells express cubilin, megalin, ClC-5, amnionless and Dab2, which are partners in the tubular machinery. Exposing human podocytes to albumin overload prompted an increase in CUBILIN, AMNIONLESS and CLCN5 gene expression. Inhibiting cubilin led to a reduction in albumin uptake, highlighting its importance in this mechanism. We demonstrated that human podocytes are committed to performing endocytosis via a receptor-mediated mechanism even in the presence of low doses of albumin. We also disclosed that protein overload first acts on the expression of the cubilin-amnionless (CUBAM) complex in these cells, then involves the ClC-5 channel, providing the first evidence for a possible role of the CUBAM complex in albumin endocytosis in human podocytes.Lisa GianeselloGiovanna PrianteMonica CeolClaudia M. RaduMoin A. SaleemPaolo SimioniLiliana TerrinFranca AnglaniDorella Del PreteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lisa Gianesello Giovanna Priante Monica Ceol Claudia M. Radu Moin A. Saleem Paolo Simioni Liliana Terrin Franca Anglani Dorella Del Prete Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
description	Abstract Albumin re-uptake is a receptor-mediated pathway located in renal proximal tubuli. There is increasing evidence of glomerular protein handling by podocytes, but little is known about the mechanism behind this process. In this study, we found that human podocytes in vitro are committed to internalizing albumin through a receptor-mediated mechanism even after exposure to low doses of albumin. We show that these cells express cubilin, megalin, ClC-5, amnionless and Dab2, which are partners in the tubular machinery. Exposing human podocytes to albumin overload prompted an increase in CUBILIN, AMNIONLESS and CLCN5 gene expression. Inhibiting cubilin led to a reduction in albumin uptake, highlighting its importance in this mechanism. We demonstrated that human podocytes are committed to performing endocytosis via a receptor-mediated mechanism even in the presence of low doses of albumin. We also disclosed that protein overload first acts on the expression of the cubilin-amnionless (CUBAM) complex in these cells, then involves the ClC-5 channel, providing the first evidence for a possible role of the CUBAM complex in albumin endocytosis in human podocytes.
format	article
author	Lisa Gianesello Giovanna Priante Monica Ceol Claudia M. Radu Moin A. Saleem Paolo Simioni Liliana Terrin Franca Anglani Dorella Del Prete
author_facet	Lisa Gianesello Giovanna Priante Monica Ceol Claudia M. Radu Moin A. Saleem Paolo Simioni Liliana Terrin Franca Anglani Dorella Del Prete
author_sort	Lisa Gianesello
title	Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
title_short	Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
title_full	Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
title_fullStr	Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
title_full_unstemmed	Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
title_sort	albumin uptake in human podocytes: a possible role for the cubilin-amnionless (cubam) complex
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/870c4846e16141b892ddc0d384672106
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Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex

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