Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex

Abstract Albumin re-uptake is a receptor-mediated pathway located in renal proximal tubuli. There is increasing evidence of glomerular protein handling by podocytes, but little is known about the mechanism behind this process. In this study, we found that human podocytes in vitro are committed to in...

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Autores principales: Lisa Gianesello, Giovanna Priante, Monica Ceol, Claudia M. Radu, Moin A. Saleem, Paolo Simioni, Liliana Terrin, Franca Anglani, Dorella Del Prete
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Publicado: Nature Portfolio 2017
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spelling oai:doaj.org-article:870c4846e16141b892ddc0d3846721062021-12-02T15:05:53ZAlbumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex10.1038/s41598-017-13789-z2045-2322https://doaj.org/article/870c4846e16141b892ddc0d3846721062017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-13789-zhttps://doaj.org/toc/2045-2322Abstract Albumin re-uptake is a receptor-mediated pathway located in renal proximal tubuli. There is increasing evidence of glomerular protein handling by podocytes, but little is known about the mechanism behind this process. In this study, we found that human podocytes in vitro are committed to internalizing albumin through a receptor-mediated mechanism even after exposure to low doses of albumin. We show that these cells express cubilin, megalin, ClC-5, amnionless and Dab2, which are partners in the tubular machinery. Exposing human podocytes to albumin overload prompted an increase in CUBILIN, AMNIONLESS and CLCN5 gene expression. Inhibiting cubilin led to a reduction in albumin uptake, highlighting its importance in this mechanism. We demonstrated that human podocytes are committed to performing endocytosis via a receptor-mediated mechanism even in the presence of low doses of albumin. We also disclosed that protein overload first acts on the expression of the cubilin-amnionless (CUBAM) complex in these cells, then involves the ClC-5 channel, providing the first evidence for a possible role of the CUBAM complex in albumin endocytosis in human podocytes.Lisa GianeselloGiovanna PrianteMonica CeolClaudia M. RaduMoin A. SaleemPaolo SimioniLiliana TerrinFranca AnglaniDorella Del PreteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Lisa Gianesello
Giovanna Priante
Monica Ceol
Claudia M. Radu
Moin A. Saleem
Paolo Simioni
Liliana Terrin
Franca Anglani
Dorella Del Prete
Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
description Abstract Albumin re-uptake is a receptor-mediated pathway located in renal proximal tubuli. There is increasing evidence of glomerular protein handling by podocytes, but little is known about the mechanism behind this process. In this study, we found that human podocytes in vitro are committed to internalizing albumin through a receptor-mediated mechanism even after exposure to low doses of albumin. We show that these cells express cubilin, megalin, ClC-5, amnionless and Dab2, which are partners in the tubular machinery. Exposing human podocytes to albumin overload prompted an increase in CUBILIN, AMNIONLESS and CLCN5 gene expression. Inhibiting cubilin led to a reduction in albumin uptake, highlighting its importance in this mechanism. We demonstrated that human podocytes are committed to performing endocytosis via a receptor-mediated mechanism even in the presence of low doses of albumin. We also disclosed that protein overload first acts on the expression of the cubilin-amnionless (CUBAM) complex in these cells, then involves the ClC-5 channel, providing the first evidence for a possible role of the CUBAM complex in albumin endocytosis in human podocytes.
format article
author Lisa Gianesello
Giovanna Priante
Monica Ceol
Claudia M. Radu
Moin A. Saleem
Paolo Simioni
Liliana Terrin
Franca Anglani
Dorella Del Prete
author_facet Lisa Gianesello
Giovanna Priante
Monica Ceol
Claudia M. Radu
Moin A. Saleem
Paolo Simioni
Liliana Terrin
Franca Anglani
Dorella Del Prete
author_sort Lisa Gianesello
title Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
title_short Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
title_full Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
title_fullStr Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
title_full_unstemmed Albumin uptake in human podocytes: a possible role for the cubilin-amnionless (CUBAM) complex
title_sort albumin uptake in human podocytes: a possible role for the cubilin-amnionless (cubam) complex
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/870c4846e16141b892ddc0d384672106
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