Changes in lung immune cell infiltrates after electric field treatment in mice

Abstract Exogenous electric fields are currently used in human therapy in a number of contexts. Interestingly, electric fields have also been shown to alter migration and function of immune cells, suggesting the potential for electric field-based immune therapy. Little is known as to the effect of e...

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Autores principales:	Sophia I. Eliseeva, Zackery A. Knowlden, Gillian MSchiralli Lester, David A. Dean, Steve N. Georas, Timothy J. Chapman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/872ea39fdb4d4a9db837f26f8ca4c51a
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spelling	oai:doaj.org-article:872ea39fdb4d4a9db837f26f8ca4c51a2021-12-02T15:23:10ZChanges in lung immune cell infiltrates after electric field treatment in mice10.1038/s41598-021-81174-y2045-2322https://doaj.org/article/872ea39fdb4d4a9db837f26f8ca4c51a2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81174-yhttps://doaj.org/toc/2045-2322Abstract Exogenous electric fields are currently used in human therapy in a number of contexts. Interestingly, electric fields have also been shown to alter migration and function of immune cells, suggesting the potential for electric field-based immune therapy. Little is known as to the effect of electric field treatment (EFT) on the lung. To determine if EFT associates with changes in lung immune cell infiltration, we used a mouse model with varying methods of EFT application and measured cells and soluble mediators using flow cytometry and cytokine/chemokine multiplex. EFT was associated with a transient increase in lung neutrophils and decrease in eosinophils in naïve mice within 2 h of treatment, accompanied by an increase in IL-6 levels. In order to test whether EFT could alter eosinophil/neutrophil recruitment in a relevant disease model, a mouse model of allergic airway inflammation was used. Four EFT doses in allergen-sensitized mice resulted in increased neutrophil and reduced eosinophil infiltrates following allergen challenge, suggesting a durable change in inflammation by EFT. Mice with allergic inflammation were analyzed by flexiVent for measures of lung function. EFT-treated mice had increased inspiratory capacity and other measures of lung function were not diminished. These data suggest EFT may be used to manipulate immune cell infiltration in the lung without affecting lung function.Sophia I. EliseevaZackery A. KnowldenGillian MSchiralli LesterDavid A. DeanSteve N. GeorasTimothy J. ChapmanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sophia I. Eliseeva Zackery A. Knowlden Gillian MSchiralli Lester David A. Dean Steve N. Georas Timothy J. Chapman Changes in lung immune cell infiltrates after electric field treatment in mice
description	Abstract Exogenous electric fields are currently used in human therapy in a number of contexts. Interestingly, electric fields have also been shown to alter migration and function of immune cells, suggesting the potential for electric field-based immune therapy. Little is known as to the effect of electric field treatment (EFT) on the lung. To determine if EFT associates with changes in lung immune cell infiltration, we used a mouse model with varying methods of EFT application and measured cells and soluble mediators using flow cytometry and cytokine/chemokine multiplex. EFT was associated with a transient increase in lung neutrophils and decrease in eosinophils in naïve mice within 2 h of treatment, accompanied by an increase in IL-6 levels. In order to test whether EFT could alter eosinophil/neutrophil recruitment in a relevant disease model, a mouse model of allergic airway inflammation was used. Four EFT doses in allergen-sensitized mice resulted in increased neutrophil and reduced eosinophil infiltrates following allergen challenge, suggesting a durable change in inflammation by EFT. Mice with allergic inflammation were analyzed by flexiVent for measures of lung function. EFT-treated mice had increased inspiratory capacity and other measures of lung function were not diminished. These data suggest EFT may be used to manipulate immune cell infiltration in the lung without affecting lung function.
format	article
author	Sophia I. Eliseeva Zackery A. Knowlden Gillian MSchiralli Lester David A. Dean Steve N. Georas Timothy J. Chapman
author_facet	Sophia I. Eliseeva Zackery A. Knowlden Gillian MSchiralli Lester David A. Dean Steve N. Georas Timothy J. Chapman
author_sort	Sophia I. Eliseeva
title	Changes in lung immune cell infiltrates after electric field treatment in mice
title_short	Changes in lung immune cell infiltrates after electric field treatment in mice
title_full	Changes in lung immune cell infiltrates after electric field treatment in mice
title_fullStr	Changes in lung immune cell infiltrates after electric field treatment in mice
title_full_unstemmed	Changes in lung immune cell infiltrates after electric field treatment in mice
title_sort	changes in lung immune cell infiltrates after electric field treatment in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/872ea39fdb4d4a9db837f26f8ca4c51a
work_keys_str_mv	AT sophiaieliseeva changesinlungimmunecellinfiltratesafterelectricfieldtreatmentinmice AT zackeryaknowlden changesinlungimmunecellinfiltratesafterelectricfieldtreatmentinmice AT gillianmschirallilester changesinlungimmunecellinfiltratesafterelectricfieldtreatmentinmice AT davidadean changesinlungimmunecellinfiltratesafterelectricfieldtreatmentinmice AT stevengeoras changesinlungimmunecellinfiltratesafterelectricfieldtreatmentinmice AT timothyjchapman changesinlungimmunecellinfiltratesafterelectricfieldtreatmentinmice
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Changes in lung immune cell infiltrates after electric field treatment in mice

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