Variations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state

Abstract Ball-milling utilizes mechanical stress to modify properties of carbon nanotubes (CNTs) including size, capping, and functionalization. Ball-milling, however, may introduce structural defects resulting in altered CNT-biomolecule interactions. Nanomaterial-biomolecule interactions result in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Achyut J. Raghavendra, Kristofer Fritz, Sherleen Fu, Jared M. Brown, Ramakrishna Podila, Jonathan H. Shannahan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8740c07a311e4fc29e16a2ff02931b6d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8740c07a311e4fc29e16a2ff02931b6d
record_format	dspace
spelling	oai:doaj.org-article:8740c07a311e4fc29e16a2ff02931b6d2021-12-02T12:32:16ZVariations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state10.1038/s41598-017-08896-w2045-2322https://doaj.org/article/8740c07a311e4fc29e16a2ff02931b6d2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08896-whttps://doaj.org/toc/2045-2322Abstract Ball-milling utilizes mechanical stress to modify properties of carbon nanotubes (CNTs) including size, capping, and functionalization. Ball-milling, however, may introduce structural defects resulting in altered CNT-biomolecule interactions. Nanomaterial-biomolecule interactions result in the formation of the biocorona (BC), which alters nanomaterial properties, function, and biological responses. The formation of the BC is governed by the nanomaterial physicochemical properties and the physiological environment. Underlying disease states such as cardiovascular disease can alter the biological milieu possibly leading to unique BC identities. In this ex vivo study, we evaluated variations in the formation of the BC on single-walled CNTs (SWCNTs) due to physicochemical alterations in structure resulting from ball-milling and variations in the environment due to the high-cholesterol disease state. Increased ball-milling time of SWCNTs resulted in enhanced structural defects. Following incubation in normal mouse serum, label-free quantitative proteomics identified differences in the biomolecular content of the BC due to the ball-milling process. Further, incubation in cholesterol-rich mouse serum resulted in the formation of unique BCs compared to SWCNTs incubated in normal serum. Our study demonstrates that the BC is modified due to physicochemical modifications such as defects induced by ball-milling and physiological disease conditions, which may result in variable biological responses.Achyut J. RaghavendraKristofer FritzSherleen FuJared M. BrownRamakrishna PodilaJonathan H. ShannahanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Achyut J. Raghavendra Kristofer Fritz Sherleen Fu Jared M. Brown Ramakrishna Podila Jonathan H. Shannahan Variations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state
description	Abstract Ball-milling utilizes mechanical stress to modify properties of carbon nanotubes (CNTs) including size, capping, and functionalization. Ball-milling, however, may introduce structural defects resulting in altered CNT-biomolecule interactions. Nanomaterial-biomolecule interactions result in the formation of the biocorona (BC), which alters nanomaterial properties, function, and biological responses. The formation of the BC is governed by the nanomaterial physicochemical properties and the physiological environment. Underlying disease states such as cardiovascular disease can alter the biological milieu possibly leading to unique BC identities. In this ex vivo study, we evaluated variations in the formation of the BC on single-walled CNTs (SWCNTs) due to physicochemical alterations in structure resulting from ball-milling and variations in the environment due to the high-cholesterol disease state. Increased ball-milling time of SWCNTs resulted in enhanced structural defects. Following incubation in normal mouse serum, label-free quantitative proteomics identified differences in the biomolecular content of the BC due to the ball-milling process. Further, incubation in cholesterol-rich mouse serum resulted in the formation of unique BCs compared to SWCNTs incubated in normal serum. Our study demonstrates that the BC is modified due to physicochemical modifications such as defects induced by ball-milling and physiological disease conditions, which may result in variable biological responses.
format	article
author	Achyut J. Raghavendra Kristofer Fritz Sherleen Fu Jared M. Brown Ramakrishna Podila Jonathan H. Shannahan
author_facet	Achyut J. Raghavendra Kristofer Fritz Sherleen Fu Jared M. Brown Ramakrishna Podila Jonathan H. Shannahan
author_sort	Achyut J. Raghavendra
title	Variations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state
title_short	Variations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state
title_full	Variations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state
title_fullStr	Variations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state
title_full_unstemmed	Variations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state
title_sort	variations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8740c07a311e4fc29e16a2ff02931b6d
work_keys_str_mv	AT achyutjraghavendra variationsinbiocoronaformationrelatedtodefectsinthestructureofsinglewalledcarbonnanotubesandthehyperlipidemicdiseasestate AT kristoferfritz variationsinbiocoronaformationrelatedtodefectsinthestructureofsinglewalledcarbonnanotubesandthehyperlipidemicdiseasestate AT sherleenfu variationsinbiocoronaformationrelatedtodefectsinthestructureofsinglewalledcarbonnanotubesandthehyperlipidemicdiseasestate AT jaredmbrown variationsinbiocoronaformationrelatedtodefectsinthestructureofsinglewalledcarbonnanotubesandthehyperlipidemicdiseasestate AT ramakrishnapodila variationsinbiocoronaformationrelatedtodefectsinthestructureofsinglewalledcarbonnanotubesandthehyperlipidemicdiseasestate AT jonathanhshannahan variationsinbiocoronaformationrelatedtodefectsinthestructureofsinglewalledcarbonnanotubesandthehyperlipidemicdiseasestate
_version_	1718394105407471616

Variations in biocorona formation related to defects in the structure of single walled carbon nanotubes and the hyperlipidemic disease state

Ejemplares similares