The Maestro (Mro) gene is dispensable for normal sexual development and fertility in mice.

The mammalian gonad arises as a bipotential primordium from which a testis or ovary develops depending on the chromosomal sex of the individual. We have previously used DNA microarrays to screen for novel genes controlling the developmental fate of the indifferent embryonic mouse gonad. Maestro (Mro...

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Autores principales:	Lee Smith, John Willan, Nick Warr, Frances A Brook, Michael Cheeseman, Richard Sharpe, Pam Siggers, Andy Greenfield
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/874626d44c6a47d0ab62ac88e316edf9
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spelling	oai:doaj.org-article:874626d44c6a47d0ab62ac88e316edf92021-11-25T06:17:55ZThe Maestro (Mro) gene is dispensable for normal sexual development and fertility in mice.1932-620310.1371/journal.pone.0004091https://doaj.org/article/874626d44c6a47d0ab62ac88e316edf92008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19116663/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The mammalian gonad arises as a bipotential primordium from which a testis or ovary develops depending on the chromosomal sex of the individual. We have previously used DNA microarrays to screen for novel genes controlling the developmental fate of the indifferent embryonic mouse gonad. Maestro (Mro), which encodes a HEAT-repeat protein, was originally identified as a gene exhibiting sexually dimorphic expression during mouse gonad development. Wholemount in situ hybridisation analysis revealed Mro to be expressed in the embryonic male gonad from approximately 11.5 days post coitum, prior to overt sexual differentiation. No significant expression was detected in female gonads at the same developmental stage. In order to address its physiological function, we have generated mice lacking Maestro using gene targeting. Male and female mice homozygous for a Mro null allele are viable and fertile. We examined gonad development in homozygous male embryos in detail and observed no differences when compared to wild-type controls. Immunohistochemical analysis of homozygous mutant testes of adult mice revealed no overt abnormalities. Expression profiling using DNA microarrays also indicated no significant differences between homozygote embryonic male gonads and controls. We conclude that Maestro is dispensable for normal male sexual development and fertility in laboratory mice; however, the Mro locus itself does have utility as a site for insertion of transgenes for future studies in the fields of sexual development and Sertoli cell function.Lee SmithJohn WillanNick WarrFrances A BrookMichael CheesemanRichard SharpePam SiggersAndy GreenfieldPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 12, p e4091 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lee Smith John Willan Nick Warr Frances A Brook Michael Cheeseman Richard Sharpe Pam Siggers Andy Greenfield The Maestro (Mro) gene is dispensable for normal sexual development and fertility in mice.
description	The mammalian gonad arises as a bipotential primordium from which a testis or ovary develops depending on the chromosomal sex of the individual. We have previously used DNA microarrays to screen for novel genes controlling the developmental fate of the indifferent embryonic mouse gonad. Maestro (Mro), which encodes a HEAT-repeat protein, was originally identified as a gene exhibiting sexually dimorphic expression during mouse gonad development. Wholemount in situ hybridisation analysis revealed Mro to be expressed in the embryonic male gonad from approximately 11.5 days post coitum, prior to overt sexual differentiation. No significant expression was detected in female gonads at the same developmental stage. In order to address its physiological function, we have generated mice lacking Maestro using gene targeting. Male and female mice homozygous for a Mro null allele are viable and fertile. We examined gonad development in homozygous male embryos in detail and observed no differences when compared to wild-type controls. Immunohistochemical analysis of homozygous mutant testes of adult mice revealed no overt abnormalities. Expression profiling using DNA microarrays also indicated no significant differences between homozygote embryonic male gonads and controls. We conclude that Maestro is dispensable for normal male sexual development and fertility in laboratory mice; however, the Mro locus itself does have utility as a site for insertion of transgenes for future studies in the fields of sexual development and Sertoli cell function.
format	article
author	Lee Smith John Willan Nick Warr Frances A Brook Michael Cheeseman Richard Sharpe Pam Siggers Andy Greenfield
author_facet	Lee Smith John Willan Nick Warr Frances A Brook Michael Cheeseman Richard Sharpe Pam Siggers Andy Greenfield
author_sort	Lee Smith
title	The Maestro (Mro) gene is dispensable for normal sexual development and fertility in mice.
title_short	The Maestro (Mro) gene is dispensable for normal sexual development and fertility in mice.
title_full	The Maestro (Mro) gene is dispensable for normal sexual development and fertility in mice.
title_fullStr	The Maestro (Mro) gene is dispensable for normal sexual development and fertility in mice.
title_full_unstemmed	The Maestro (Mro) gene is dispensable for normal sexual development and fertility in mice.
title_sort	maestro (mro) gene is dispensable for normal sexual development and fertility in mice.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/874626d44c6a47d0ab62ac88e316edf9
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The Maestro (Mro) gene is dispensable for normal sexual development and fertility in mice.

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