Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells

Abstract Here, we report an atomic force microscopy (AFM)-based imaging method for resolving the fine nanostructures (e.g., fenestrations) in the membranes of live primary murine liver sinusoidal endothelial cells (LSECs). From data on topographical and nanomechanical properties of the selected cell...

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Autores principales:	B. Zapotoczny, K. Szafranska, K. Owczarczyk, E. Kus, S. Chlopicki, M. Szymonski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/874bf54c82ea442c9e65a05c2161e1a4
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spelling	oai:doaj.org-article:874bf54c82ea442c9e65a05c2161e1a42021-12-02T15:06:06ZAtomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells10.1038/s41598-017-08555-02045-2322https://doaj.org/article/874bf54c82ea442c9e65a05c2161e1a42017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08555-0https://doaj.org/toc/2045-2322Abstract Here, we report an atomic force microscopy (AFM)-based imaging method for resolving the fine nanostructures (e.g., fenestrations) in the membranes of live primary murine liver sinusoidal endothelial cells (LSECs). From data on topographical and nanomechanical properties of the selected cell areas collected within 1 min, we traced the dynamic rearrangement of the cell actin cytoskeleton connected with the formation or closing of cell fenestrations, both in non-stimulated LSECs as well as in response to cytochalasin B and antimycin A. In conclusion, AFM-based imaging permitted the near real-time measurements of dynamic changes in fenestrations in live LSECs.B. ZapotocznyK. SzafranskaK. OwczarczykE. KusS. ChlopickiM. SzymonskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q B. Zapotoczny K. Szafranska K. Owczarczyk E. Kus S. Chlopicki M. Szymonski Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
description	Abstract Here, we report an atomic force microscopy (AFM)-based imaging method for resolving the fine nanostructures (e.g., fenestrations) in the membranes of live primary murine liver sinusoidal endothelial cells (LSECs). From data on topographical and nanomechanical properties of the selected cell areas collected within 1 min, we traced the dynamic rearrangement of the cell actin cytoskeleton connected with the formation or closing of cell fenestrations, both in non-stimulated LSECs as well as in response to cytochalasin B and antimycin A. In conclusion, AFM-based imaging permitted the near real-time measurements of dynamic changes in fenestrations in live LSECs.
format	article
author	B. Zapotoczny K. Szafranska K. Owczarczyk E. Kus S. Chlopicki M. Szymonski
author_facet	B. Zapotoczny K. Szafranska K. Owczarczyk E. Kus S. Chlopicki M. Szymonski
author_sort	B. Zapotoczny
title	Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title_short	Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title_full	Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title_fullStr	Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title_full_unstemmed	Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
title_sort	atomic force microscopy reveals the dynamic morphology of fenestrations in live liver sinusoidal endothelial cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/874bf54c82ea442c9e65a05c2161e1a4
work_keys_str_mv	AT bzapotoczny atomicforcemicroscopyrevealsthedynamicmorphologyoffenestrationsinliveliversinusoidalendothelialcells AT kszafranska atomicforcemicroscopyrevealsthedynamicmorphologyoffenestrationsinliveliversinusoidalendothelialcells AT kowczarczyk atomicforcemicroscopyrevealsthedynamicmorphologyoffenestrationsinliveliversinusoidalendothelialcells AT ekus atomicforcemicroscopyrevealsthedynamicmorphologyoffenestrationsinliveliversinusoidalendothelialcells AT schlopicki atomicforcemicroscopyrevealsthedynamicmorphologyoffenestrationsinliveliversinusoidalendothelialcells AT mszymonski atomicforcemicroscopyrevealsthedynamicmorphologyoffenestrationsinliveliversinusoidalendothelialcells
_version_	1718388595243352064

Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells

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