A new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in C. elegans.

The cohesin complex is required for the cohesion of sister chromatids and for correct segregation during mitosis and meiosis. Crossover recombination, together with cohesion, is essential for the disjunction of homologous chromosomes during the first meiotic division. Cohesin has been implicated in...

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Autores principales:	Antoine Baudrimont, Alexandra Penkner, Alexander Woglar, Yasmine M Mamnun, Margot Hulek, Cathrin Struck, Ralf Schnabel, Josef Loidl, Verena Jantsch
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/87538796aa384083a94e6747e481cb12
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spelling	oai:doaj.org-article:87538796aa384083a94e6747e481cb122021-11-04T06:08:08ZA new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in C. elegans.1932-620310.1371/journal.pone.0024799https://doaj.org/article/87538796aa384083a94e6747e481cb122011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21957461/?tool=EBIhttps://doaj.org/toc/1932-6203The cohesin complex is required for the cohesion of sister chromatids and for correct segregation during mitosis and meiosis. Crossover recombination, together with cohesion, is essential for the disjunction of homologous chromosomes during the first meiotic division. Cohesin has been implicated in facilitating recombinational repair of DNA lesions via the sister chromatid. Here, we made use of a new temperature-sensitive mutation in the Caenorhabditis elegans SMC-3 protein to study the role of cohesin in the repair of DNA double-strand breaks (DSBs) and hence in meiotic crossing over. We report that attenuation of cohesin was associated with extensive SPO-11-dependent chromosome fragmentation, which is representative of unrepaired DSBs. We also found that attenuated cohesin likely increased the number of DSBs and eliminated the need of MRE-11 and RAD-50 for DSB formation in C. elegans, which suggests a role for the MRN complex in making cohesin-loaded chromatin susceptible to meiotic DSBs. Notably, in spite of largely intact sister chromatid cohesion, backup DSB repair via the sister chromatid was mostly impaired. We also found that weakened cohesins affected mitotic repair of DSBs by homologous recombination, whereas NHEJ repair was not affected. Our data suggest that recombinational DNA repair makes higher demands on cohesins than does chromosome segregation.Antoine BaudrimontAlexandra PenknerAlexander WoglarYasmine M MamnunMargot HulekCathrin StruckRalf SchnabelJosef LoidlVerena JantschPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24799 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antoine Baudrimont Alexandra Penkner Alexander Woglar Yasmine M Mamnun Margot Hulek Cathrin Struck Ralf Schnabel Josef Loidl Verena Jantsch A new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in C. elegans.
description	The cohesin complex is required for the cohesion of sister chromatids and for correct segregation during mitosis and meiosis. Crossover recombination, together with cohesion, is essential for the disjunction of homologous chromosomes during the first meiotic division. Cohesin has been implicated in facilitating recombinational repair of DNA lesions via the sister chromatid. Here, we made use of a new temperature-sensitive mutation in the Caenorhabditis elegans SMC-3 protein to study the role of cohesin in the repair of DNA double-strand breaks (DSBs) and hence in meiotic crossing over. We report that attenuation of cohesin was associated with extensive SPO-11-dependent chromosome fragmentation, which is representative of unrepaired DSBs. We also found that attenuated cohesin likely increased the number of DSBs and eliminated the need of MRE-11 and RAD-50 for DSB formation in C. elegans, which suggests a role for the MRN complex in making cohesin-loaded chromatin susceptible to meiotic DSBs. Notably, in spite of largely intact sister chromatid cohesion, backup DSB repair via the sister chromatid was mostly impaired. We also found that weakened cohesins affected mitotic repair of DSBs by homologous recombination, whereas NHEJ repair was not affected. Our data suggest that recombinational DNA repair makes higher demands on cohesins than does chromosome segregation.
format	article
author	Antoine Baudrimont Alexandra Penkner Alexander Woglar Yasmine M Mamnun Margot Hulek Cathrin Struck Ralf Schnabel Josef Loidl Verena Jantsch
author_facet	Antoine Baudrimont Alexandra Penkner Alexander Woglar Yasmine M Mamnun Margot Hulek Cathrin Struck Ralf Schnabel Josef Loidl Verena Jantsch
author_sort	Antoine Baudrimont
title	A new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in C. elegans.
title_short	A new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in C. elegans.
title_full	A new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in C. elegans.
title_fullStr	A new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in C. elegans.
title_full_unstemmed	A new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in C. elegans.
title_sort	new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in c. elegans.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/87538796aa384083a94e6747e481cb12
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A new thermosensitive smc-3 allele reveals involvement of cohesin in homologous recombination in C. elegans.

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