Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station.

The "GENARA A" experiment was designed to monitor global changes in the proteome of membranes of Arabidopsis thaliana seedlings subjected to microgravity on board the International Space Station (ISS). For this purpose, 12-day-old seedlings were grown either in space, in the European Modul...

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Autores principales:	Christian Mazars, Christian Brière, Sabine Grat, Carole Pichereaux, Michel Rossignol, Veronica Pereda-Loth, Brigitte Eche, Elodie Boucheron-Dubuisson, Isabel Le Disquet, Francisco Javier Medina, Annick Graziana, Eugénie Carnero-Diaz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8757db92704e49e1ac98bf9d9185f1d5
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spelling	oai:doaj.org-article:8757db92704e49e1ac98bf9d9185f1d52021-11-18T08:28:47ZMicrogravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station.1932-620310.1371/journal.pone.0091814https://doaj.org/article/8757db92704e49e1ac98bf9d9185f1d52014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24618597/?tool=EBIhttps://doaj.org/toc/1932-6203The "GENARA A" experiment was designed to monitor global changes in the proteome of membranes of Arabidopsis thaliana seedlings subjected to microgravity on board the International Space Station (ISS). For this purpose, 12-day-old seedlings were grown either in space, in the European Modular Cultivation System (EMCS) under microgravity or on a 1 g centrifuge, or on the ground. Proteins associated to membranes were selectively extracted from microsomes and identified and quantified through LC-MS-MS using a label-free method. Among the 1484 proteins identified and quantified in the 3 conditions mentioned above, 80 membrane-associated proteins were significantly more abundant in seedlings grown under microgravity in space than under 1 g (space and ground) and 69 were less abundant. Clustering of these proteins according to their predicted function indicates that proteins associated to auxin metabolism and trafficking were depleted in the microsomal fraction in µg space conditions, whereas proteins associated to stress responses, defence and metabolism were more abundant in µg than in 1 g indicating that microgravity is perceived by plants as a stressful environment. These results clearly indicate that a global membrane proteomics approach gives a snapshot of the cell status and its signaling activity in response to microgravity and highlight the major processes affected.Christian MazarsChristian BrièreSabine GratCarole PichereauxMichel RossignolVeronica Pereda-LothBrigitte EcheElodie Boucheron-DubuissonIsabel Le DisquetFrancisco Javier MedinaAnnick GrazianaEugénie Carnero-DiazPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91814 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Mazars Christian Brière Sabine Grat Carole Pichereaux Michel Rossignol Veronica Pereda-Loth Brigitte Eche Elodie Boucheron-Dubuisson Isabel Le Disquet Francisco Javier Medina Annick Graziana Eugénie Carnero-Diaz Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station.
description	The "GENARA A" experiment was designed to monitor global changes in the proteome of membranes of Arabidopsis thaliana seedlings subjected to microgravity on board the International Space Station (ISS). For this purpose, 12-day-old seedlings were grown either in space, in the European Modular Cultivation System (EMCS) under microgravity or on a 1 g centrifuge, or on the ground. Proteins associated to membranes were selectively extracted from microsomes and identified and quantified through LC-MS-MS using a label-free method. Among the 1484 proteins identified and quantified in the 3 conditions mentioned above, 80 membrane-associated proteins were significantly more abundant in seedlings grown under microgravity in space than under 1 g (space and ground) and 69 were less abundant. Clustering of these proteins according to their predicted function indicates that proteins associated to auxin metabolism and trafficking were depleted in the microsomal fraction in µg space conditions, whereas proteins associated to stress responses, defence and metabolism were more abundant in µg than in 1 g indicating that microgravity is perceived by plants as a stressful environment. These results clearly indicate that a global membrane proteomics approach gives a snapshot of the cell status and its signaling activity in response to microgravity and highlight the major processes affected.
format	article
author	Christian Mazars Christian Brière Sabine Grat Carole Pichereaux Michel Rossignol Veronica Pereda-Loth Brigitte Eche Elodie Boucheron-Dubuisson Isabel Le Disquet Francisco Javier Medina Annick Graziana Eugénie Carnero-Diaz
author_facet	Christian Mazars Christian Brière Sabine Grat Carole Pichereaux Michel Rossignol Veronica Pereda-Loth Brigitte Eche Elodie Boucheron-Dubuisson Isabel Le Disquet Francisco Javier Medina Annick Graziana Eugénie Carnero-Diaz
author_sort	Christian Mazars
title	Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station.
title_short	Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station.
title_full	Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station.
title_fullStr	Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station.
title_full_unstemmed	Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station.
title_sort	microgravity induces changes in microsome-associated proteins of arabidopsis seedlings grown on board the international space station.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/8757db92704e49e1ac98bf9d9185f1d5
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Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station.

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