Methylation of BRCA1 promoter region is associated with unfavorable prognosis in women with early-stage breast cancer.

BRCA1-associated breast cancers are associated with particular features such as early onset, poor histological differentiation, and hormone receptor negativity. Previous studies conducted in Taiwanese population showed that the mutation of BRCA1 gene does not play a significant role in the occurrenc...

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Autores principales:	Nicholas C Hsu, Ya-Fang Huang, Kazunari K Yokoyama, Pei-Yi Chu, Fang-Ming Chen, Ming-Feng Hou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/875a407dc2c645dea6f376175d4717ee
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spelling	oai:doaj.org-article:875a407dc2c645dea6f376175d4717ee2021-11-18T07:58:26ZMethylation of BRCA1 promoter region is associated with unfavorable prognosis in women with early-stage breast cancer.1932-620310.1371/journal.pone.0056256https://doaj.org/article/875a407dc2c645dea6f376175d4717ee2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23405268/?tool=EBIhttps://doaj.org/toc/1932-6203BRCA1-associated breast cancers are associated with particular features such as early onset, poor histological differentiation, and hormone receptor negativity. Previous studies conducted in Taiwanese population showed that the mutation of BRCA1 gene does not play a significant role in the occurrence of breast cancer. The present study explored methylation of BRCA1 promoter and its relationship to clinical features and outcome in Taiwanese breast cancer patients. Tumor specimens from a cohort of 139 early-stage breast cancer patients were obtained during surgery before adjuvant treatment for DNA extraction. Methylation of BRCA1 promoter region was determined by methylation-specific PCR and the results were related to clinical features and outcome of patients using statistical analysis. Methylation of the BRCA1 promoter was detected in 78 (56%) of the 139 tumors. Chi-square analysis indicated that BRCA1 promoter methylation correlated significantly with triple-negative (ER-/PR-/HER2-) status of breast cancer patients (p = 0.041). The Kaplan-Meier method showed that BRCA1 promoter methylation was significantly associated with poor overall survival (p = 0.026) and disease-free survival (p = 0.001). Multivariate analysis which incorporated variables of patients' age, tumor size, grade, and lymph node metastasis revealed that BRCA1 promoter methylation was associated with overall survival (p = 0.027; hazard ratio, 16.38) and disease-free survival (p = 0.003; hazard ratio, 12.19) [corrected].Our findings underscore the clinical relevance of the methylation of BRCA1 promoter in Taiwanese patients with early-stage breast cancer.Nicholas C HsuYa-Fang HuangKazunari K YokoyamaPei-Yi ChuFang-Ming ChenMing-Feng HouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56256 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicholas C Hsu Ya-Fang Huang Kazunari K Yokoyama Pei-Yi Chu Fang-Ming Chen Ming-Feng Hou Methylation of BRCA1 promoter region is associated with unfavorable prognosis in women with early-stage breast cancer.
description	BRCA1-associated breast cancers are associated with particular features such as early onset, poor histological differentiation, and hormone receptor negativity. Previous studies conducted in Taiwanese population showed that the mutation of BRCA1 gene does not play a significant role in the occurrence of breast cancer. The present study explored methylation of BRCA1 promoter and its relationship to clinical features and outcome in Taiwanese breast cancer patients. Tumor specimens from a cohort of 139 early-stage breast cancer patients were obtained during surgery before adjuvant treatment for DNA extraction. Methylation of BRCA1 promoter region was determined by methylation-specific PCR and the results were related to clinical features and outcome of patients using statistical analysis. Methylation of the BRCA1 promoter was detected in 78 (56%) of the 139 tumors. Chi-square analysis indicated that BRCA1 promoter methylation correlated significantly with triple-negative (ER-/PR-/HER2-) status of breast cancer patients (p = 0.041). The Kaplan-Meier method showed that BRCA1 promoter methylation was significantly associated with poor overall survival (p = 0.026) and disease-free survival (p = 0.001). Multivariate analysis which incorporated variables of patients' age, tumor size, grade, and lymph node metastasis revealed that BRCA1 promoter methylation was associated with overall survival (p = 0.027; hazard ratio, 16.38) and disease-free survival (p = 0.003; hazard ratio, 12.19) [corrected].Our findings underscore the clinical relevance of the methylation of BRCA1 promoter in Taiwanese patients with early-stage breast cancer.
format	article
author	Nicholas C Hsu Ya-Fang Huang Kazunari K Yokoyama Pei-Yi Chu Fang-Ming Chen Ming-Feng Hou
author_facet	Nicholas C Hsu Ya-Fang Huang Kazunari K Yokoyama Pei-Yi Chu Fang-Ming Chen Ming-Feng Hou
author_sort	Nicholas C Hsu
title	Methylation of BRCA1 promoter region is associated with unfavorable prognosis in women with early-stage breast cancer.
title_short	Methylation of BRCA1 promoter region is associated with unfavorable prognosis in women with early-stage breast cancer.
title_full	Methylation of BRCA1 promoter region is associated with unfavorable prognosis in women with early-stage breast cancer.
title_fullStr	Methylation of BRCA1 promoter region is associated with unfavorable prognosis in women with early-stage breast cancer.
title_full_unstemmed	Methylation of BRCA1 promoter region is associated with unfavorable prognosis in women with early-stage breast cancer.
title_sort	methylation of brca1 promoter region is associated with unfavorable prognosis in women with early-stage breast cancer.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/875a407dc2c645dea6f376175d4717ee
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Methylation of BRCA1 promoter region is associated with unfavorable prognosis in women with early-stage breast cancer.

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