A New Murine Model for Gastrointestinal Anthrax Infection.

The scientific community has been restricted by the lack of a practical and informative animal model of gastrointestinal infection with vegetative Bacillus anthracis. We herein report the development of a murine model of gastrointestinal anthrax infection by gavage of vegetative Sterne strain of Bac...

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Autores principales:	Tao Xie, Chen Sun, Kadriye Uslu, Roger D Auth, Hui Fang, Weiming Ouyang, David M Frucht
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/875c123228634e03bc0f917e3b83e803
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spelling	oai:doaj.org-article:875c123228634e03bc0f917e3b83e8032021-11-18T07:41:17ZA New Murine Model for Gastrointestinal Anthrax Infection.1932-620310.1371/journal.pone.0066943https://doaj.org/article/875c123228634e03bc0f917e3b83e8032013-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0066943https://doaj.org/toc/1932-6203The scientific community has been restricted by the lack of a practical and informative animal model of gastrointestinal infection with vegetative Bacillus anthracis. We herein report the development of a murine model of gastrointestinal anthrax infection by gavage of vegetative Sterne strain of Bacillus anthracis into the complement-deficient A/J mouse strain. Mice infected in this manner developed lethal infections in a dose-dependent manner and died 30 h-5 d following gavage. Histological findings were consistent with penetration and growth of the bacilli within the intestinal villi, with subsequent dissemination into major organs including the spleen, liver, kidney and lung. Blood cultures confirmed anthrax bacteremia in all moribund animals, with approximately 1/3 showing co-infection with commensal enteric organisms. However, no evidence of immune activation was observed during infection. Time-course experiments revealed early compromise of the intestinal epithelium, characterized by villus blunting and ulceration in the ileum and jejunum. A decrease in body temperature was most predictive of near-term lethality. Antibiotic treatment of infected animals 24 h following high-dose bacterial gavage protected all animals, demonstrating the utility of this animal model in evaluating potential therapeutics.Tao XieChen SunKadriye UsluRoger D AuthHui FangWeiming OuyangDavid M FruchtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66943 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tao Xie Chen Sun Kadriye Uslu Roger D Auth Hui Fang Weiming Ouyang David M Frucht A New Murine Model for Gastrointestinal Anthrax Infection.
description	The scientific community has been restricted by the lack of a practical and informative animal model of gastrointestinal infection with vegetative Bacillus anthracis. We herein report the development of a murine model of gastrointestinal anthrax infection by gavage of vegetative Sterne strain of Bacillus anthracis into the complement-deficient A/J mouse strain. Mice infected in this manner developed lethal infections in a dose-dependent manner and died 30 h-5 d following gavage. Histological findings were consistent with penetration and growth of the bacilli within the intestinal villi, with subsequent dissemination into major organs including the spleen, liver, kidney and lung. Blood cultures confirmed anthrax bacteremia in all moribund animals, with approximately 1/3 showing co-infection with commensal enteric organisms. However, no evidence of immune activation was observed during infection. Time-course experiments revealed early compromise of the intestinal epithelium, characterized by villus blunting and ulceration in the ileum and jejunum. A decrease in body temperature was most predictive of near-term lethality. Antibiotic treatment of infected animals 24 h following high-dose bacterial gavage protected all animals, demonstrating the utility of this animal model in evaluating potential therapeutics.
format	article
author	Tao Xie Chen Sun Kadriye Uslu Roger D Auth Hui Fang Weiming Ouyang David M Frucht
author_facet	Tao Xie Chen Sun Kadriye Uslu Roger D Auth Hui Fang Weiming Ouyang David M Frucht
author_sort	Tao Xie
title	A New Murine Model for Gastrointestinal Anthrax Infection.
title_short	A New Murine Model for Gastrointestinal Anthrax Infection.
title_full	A New Murine Model for Gastrointestinal Anthrax Infection.
title_fullStr	A New Murine Model for Gastrointestinal Anthrax Infection.
title_full_unstemmed	A New Murine Model for Gastrointestinal Anthrax Infection.
title_sort	new murine model for gastrointestinal anthrax infection.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/875c123228634e03bc0f917e3b83e803
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A New Murine Model for Gastrointestinal Anthrax Infection.

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