Immunohistochemical Detection of Vasa Antigen and Apoptosis-Related DNA Fragmentation in Ovaries of Sheep Fetuses Prenatally Exposed to Vitamin D Deficiency

The primordial germ cells (PGCs) in female animals are comprised of diplotene oocytes arrested in the first meiotic prophase. Expression of Vasa is one of the key factors required for subsequent resumption of development and recruitment of PGCs into the growing follicle class. Since vitamin D regula...

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Autores principales:	Mbegbu Edmund C., Obidike Ikechukwu R., Fouladi-Nashta Ali A.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2019
Materias:	apoptosis ovary sheep vasa vitamin d deficiency Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/875f2ae5ac594c47840c7071dfe616bd
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spelling	oai:doaj.org-article:875f2ae5ac594c47840c7071dfe616bd2021-11-17T21:27:53ZImmunohistochemical Detection of Vasa Antigen and Apoptosis-Related DNA Fragmentation in Ovaries of Sheep Fetuses Prenatally Exposed to Vitamin D Deficiency1820-744810.2478/acve-2019-0022https://doaj.org/article/875f2ae5ac594c47840c7071dfe616bd2019-09-01T00:00:00Zhttps://doi.org/10.2478/acve-2019-0022https://doaj.org/toc/1820-7448The primordial germ cells (PGCs) in female animals are comprised of diplotene oocytes arrested in the first meiotic prophase. Expression of Vasa is one of the key factors required for subsequent resumption of development and recruitment of PGCs into the growing follicle class. Since vitamin D regulates recruitment of PGCs and developmental competence of ovarian follicles, this study was designed to investigate the expression of Vasa and rate of apoptosis in foetal ovaries prenatally restricted from dietary vitamin D. Nineteen sexually mature Welsh mountain ewes were randomly assigned to vitamin D deficient (VDD) and vitamin D control (VDC) diets from 17d before mating, up to 125d of gestation, when fetal ovaries were collected and fixed in formalin for immunohistochemistry and TUNEL assay. VDD ovaries had fewer healthy oocytes that could stain positive for Vasa as well as a lower integrated density value for DAB staining intensity. Conversely, TUNNEL staining in VDD animals showed a higher integrated density value and percentage of affected area (P<0.05). The present findings indicate that Vasa expression is decreased, while the rate of apoptosis increased in VDD fetal ovaries, and this may adversely affect resumption of growth and development of PGCs reserve.Mbegbu Edmund C.Obidike Ikechukwu R.Fouladi-Nashta Ali A.Sciendoarticleapoptosisovarysheepvasavitamin d deficiencyVeterinary medicineSF600-1100ENActa Veterinaria, Vol 69, Iss 3, Pp 262-274 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis ovary sheep vasa vitamin d deficiency Veterinary medicine SF600-1100
spellingShingle	apoptosis ovary sheep vasa vitamin d deficiency Veterinary medicine SF600-1100 Mbegbu Edmund C. Obidike Ikechukwu R. Fouladi-Nashta Ali A. Immunohistochemical Detection of Vasa Antigen and Apoptosis-Related DNA Fragmentation in Ovaries of Sheep Fetuses Prenatally Exposed to Vitamin D Deficiency
description	The primordial germ cells (PGCs) in female animals are comprised of diplotene oocytes arrested in the first meiotic prophase. Expression of Vasa is one of the key factors required for subsequent resumption of development and recruitment of PGCs into the growing follicle class. Since vitamin D regulates recruitment of PGCs and developmental competence of ovarian follicles, this study was designed to investigate the expression of Vasa and rate of apoptosis in foetal ovaries prenatally restricted from dietary vitamin D. Nineteen sexually mature Welsh mountain ewes were randomly assigned to vitamin D deficient (VDD) and vitamin D control (VDC) diets from 17d before mating, up to 125d of gestation, when fetal ovaries were collected and fixed in formalin for immunohistochemistry and TUNEL assay. VDD ovaries had fewer healthy oocytes that could stain positive for Vasa as well as a lower integrated density value for DAB staining intensity. Conversely, TUNNEL staining in VDD animals showed a higher integrated density value and percentage of affected area (P<0.05). The present findings indicate that Vasa expression is decreased, while the rate of apoptosis increased in VDD fetal ovaries, and this may adversely affect resumption of growth and development of PGCs reserve.
format	article
author	Mbegbu Edmund C. Obidike Ikechukwu R. Fouladi-Nashta Ali A.
author_facet	Mbegbu Edmund C. Obidike Ikechukwu R. Fouladi-Nashta Ali A.
author_sort	Mbegbu Edmund C.
title	Immunohistochemical Detection of Vasa Antigen and Apoptosis-Related DNA Fragmentation in Ovaries of Sheep Fetuses Prenatally Exposed to Vitamin D Deficiency
title_short	Immunohistochemical Detection of Vasa Antigen and Apoptosis-Related DNA Fragmentation in Ovaries of Sheep Fetuses Prenatally Exposed to Vitamin D Deficiency
title_full	Immunohistochemical Detection of Vasa Antigen and Apoptosis-Related DNA Fragmentation in Ovaries of Sheep Fetuses Prenatally Exposed to Vitamin D Deficiency
title_fullStr	Immunohistochemical Detection of Vasa Antigen and Apoptosis-Related DNA Fragmentation in Ovaries of Sheep Fetuses Prenatally Exposed to Vitamin D Deficiency
title_full_unstemmed	Immunohistochemical Detection of Vasa Antigen and Apoptosis-Related DNA Fragmentation in Ovaries of Sheep Fetuses Prenatally Exposed to Vitamin D Deficiency
title_sort	immunohistochemical detection of vasa antigen and apoptosis-related dna fragmentation in ovaries of sheep fetuses prenatally exposed to vitamin d deficiency
publisher	Sciendo
publishDate	2019
url	https://doaj.org/article/875f2ae5ac594c47840c7071dfe616bd
work_keys_str_mv	AT mbegbuedmundc immunohistochemicaldetectionofvasaantigenandapoptosisrelateddnafragmentationinovariesofsheepfetusesprenatallyexposedtovitaminddeficiency AT obidikeikechukwur immunohistochemicaldetectionofvasaantigenandapoptosisrelateddnafragmentationinovariesofsheepfetusesprenatallyexposedtovitaminddeficiency AT fouladinashtaalia immunohistochemicaldetectionofvasaantigenandapoptosisrelateddnafragmentationinovariesofsheepfetusesprenatallyexposedtovitaminddeficiency
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Immunohistochemical Detection of Vasa Antigen and Apoptosis-Related DNA Fragmentation in Ovaries of Sheep Fetuses Prenatally Exposed to Vitamin D Deficiency

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