Berberine Influences the Survival of Fat Grafting by Inhibiting Autophagy and Apoptosis of Human Adipose Derived Mesenchymal Stem Cells

Hao Pang, Yongting Zhou, Jie Wang, Hao Wu, Xueyi Liu, Feng Gao, Zhibo Xiao Department of Plastic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, People’s Republic of ChinaCorrespondence: Zhibo XiaoDepartment of Plastic Surgery, The Second Affiliate...

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Autores principales:	Pang H, Zhou Y, Wang J, Wu H, Liu X, Gao F, Xiao Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	adipose derived mesenchymal stem cells berberine autophagy apoptosis fat grafting Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/8760a940eaea45e2ba015b96c4aa5132
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spelling	oai:doaj.org-article:8760a940eaea45e2ba015b96c4aa51322021-11-30T18:50:36ZBerberine Influences the Survival of Fat Grafting by Inhibiting Autophagy and Apoptosis of Human Adipose Derived Mesenchymal Stem Cells1177-8881https://doaj.org/article/8760a940eaea45e2ba015b96c4aa51322021-11-01T00:00:00Zhttps://www.dovepress.com/berberine-influences-the-survival-of-fat-grafting-by-inhibiting-autoph-peer-reviewed-fulltext-article-DDDThttps://doaj.org/toc/1177-8881Hao Pang, Yongting Zhou, Jie Wang, Hao Wu, Xueyi Liu, Feng Gao, Zhibo Xiao Department of Plastic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, People’s Republic of ChinaCorrespondence: Zhibo XiaoDepartment of Plastic Surgery, The Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu Avenue, Harbin, Heilongjiang, 150086, People’s Republic of ChinaEmail xiaozhibodoctor@126.comObjective: Human adipose-derived mesenchymal stem cells (ADSCs) have the potential to be applied to solid organ treatments. However, tissue regeneration is limited by the death of transplanted cells. Ischemia is the main cause of the poor outcome. This study aimed to investigate the effect of berberine (BBR) on ADSCs after fat grafting.Methods: The antioxidant BBR on apoptosis and autophagy of ADSCs in vitro ischemia model was induced by hypoxia and serum deprivation (HY/SD). The autophagy promoter rapamycin and autophagy inhibitor 3-MA were incubated separately to investigate the crosstalk between autophagy and apoptosis. Pathway inhibitors further verified whether the autophagy and apoptosis were regulated by AMPK/mTor signaling pathway. Fat survival, fibrosis, level of inflammatory cell infiltration, and the effect of angiogenesis after BBR treatment were observed in vivo.Results: BBR could reduce ROS production and reverse the decreasing cell survival rate. HY/SD would induce apoptosis and autophagy in ADSCs, and BBR could alleviate these processes. After interfering with the level of autophagy, we also proved that apoptosis was regulated by autophagy and changed accordingly. The results also indicated that BBR could protect against autophagy and apoptosis of ADSCs through AMPK/mTor pathway. The treated human-derived adipose tissue was transplanted into BALB/c nude mice, and with the intervention of BBR, the fat grafting had a higher survival rate, lower inflammatory cell infiltration and fibrosis level.Conclusion: Our present study revealed that BBR was a promising anti-autophagy and apoptosis agent for improving the survival rate of ADSCs during cell transplantation.Keywords: adipose derived mesenchymal stem cells, berberine, autophagy, apoptosis, fat graftingPang HZhou YWang JWu HLiu XGao FXiao ZDove Medical Pressarticleadipose derived mesenchymal stem cellsberberineautophagyapoptosisfat graftingTherapeutics. PharmacologyRM1-950ENDrug Design, Development and Therapy, Vol Volume 15, Pp 4795-4809 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose derived mesenchymal stem cells berberine autophagy apoptosis fat grafting Therapeutics. Pharmacology RM1-950
spellingShingle	adipose derived mesenchymal stem cells berberine autophagy apoptosis fat grafting Therapeutics. Pharmacology RM1-950 Pang H Zhou Y Wang J Wu H Liu X Gao F Xiao Z Berberine Influences the Survival of Fat Grafting by Inhibiting Autophagy and Apoptosis of Human Adipose Derived Mesenchymal Stem Cells
description	Hao Pang, Yongting Zhou, Jie Wang, Hao Wu, Xueyi Liu, Feng Gao, Zhibo Xiao Department of Plastic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, People’s Republic of ChinaCorrespondence: Zhibo XiaoDepartment of Plastic Surgery, The Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu Avenue, Harbin, Heilongjiang, 150086, People’s Republic of ChinaEmail xiaozhibodoctor@126.comObjective: Human adipose-derived mesenchymal stem cells (ADSCs) have the potential to be applied to solid organ treatments. However, tissue regeneration is limited by the death of transplanted cells. Ischemia is the main cause of the poor outcome. This study aimed to investigate the effect of berberine (BBR) on ADSCs after fat grafting.Methods: The antioxidant BBR on apoptosis and autophagy of ADSCs in vitro ischemia model was induced by hypoxia and serum deprivation (HY/SD). The autophagy promoter rapamycin and autophagy inhibitor 3-MA were incubated separately to investigate the crosstalk between autophagy and apoptosis. Pathway inhibitors further verified whether the autophagy and apoptosis were regulated by AMPK/mTor signaling pathway. Fat survival, fibrosis, level of inflammatory cell infiltration, and the effect of angiogenesis after BBR treatment were observed in vivo.Results: BBR could reduce ROS production and reverse the decreasing cell survival rate. HY/SD would induce apoptosis and autophagy in ADSCs, and BBR could alleviate these processes. After interfering with the level of autophagy, we also proved that apoptosis was regulated by autophagy and changed accordingly. The results also indicated that BBR could protect against autophagy and apoptosis of ADSCs through AMPK/mTor pathway. The treated human-derived adipose tissue was transplanted into BALB/c nude mice, and with the intervention of BBR, the fat grafting had a higher survival rate, lower inflammatory cell infiltration and fibrosis level.Conclusion: Our present study revealed that BBR was a promising anti-autophagy and apoptosis agent for improving the survival rate of ADSCs during cell transplantation.Keywords: adipose derived mesenchymal stem cells, berberine, autophagy, apoptosis, fat grafting
format	article
author	Pang H Zhou Y Wang J Wu H Liu X Gao F Xiao Z
author_facet	Pang H Zhou Y Wang J Wu H Liu X Gao F Xiao Z
author_sort	Pang H
title	Berberine Influences the Survival of Fat Grafting by Inhibiting Autophagy and Apoptosis of Human Adipose Derived Mesenchymal Stem Cells
title_short	Berberine Influences the Survival of Fat Grafting by Inhibiting Autophagy and Apoptosis of Human Adipose Derived Mesenchymal Stem Cells
title_full	Berberine Influences the Survival of Fat Grafting by Inhibiting Autophagy and Apoptosis of Human Adipose Derived Mesenchymal Stem Cells
title_fullStr	Berberine Influences the Survival of Fat Grafting by Inhibiting Autophagy and Apoptosis of Human Adipose Derived Mesenchymal Stem Cells
title_full_unstemmed	Berberine Influences the Survival of Fat Grafting by Inhibiting Autophagy and Apoptosis of Human Adipose Derived Mesenchymal Stem Cells
title_sort	berberine influences the survival of fat grafting by inhibiting autophagy and apoptosis of human adipose derived mesenchymal stem cells
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/8760a940eaea45e2ba015b96c4aa5132
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Berberine Influences the Survival of Fat Grafting by Inhibiting Autophagy and Apoptosis of Human Adipose Derived Mesenchymal Stem Cells

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