Host-Adaptation of Burkholderia pseudomallei Alters Metabolism and Virulence: a Global Proteome Analysis

Abstract Little is known about the evolution, adaptation and pathogenesis of Burkholderia pseudomallei within host during acute melioidosis infection. Melioidosis is a potential life threatening disease contracted through inhalation, ingestion, inoculation or direct entry of the organism into the bl...

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Autores principales:	Vanitha Mariappan, Kumutha Malar Vellasamy, Jamuna Vadivelu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8768e21642d94aad8c0616d20249b60e
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spelling	oai:doaj.org-article:8768e21642d94aad8c0616d20249b60e2021-12-02T16:06:56ZHost-Adaptation of Burkholderia pseudomallei Alters Metabolism and Virulence: a Global Proteome Analysis10.1038/s41598-017-09373-02045-2322https://doaj.org/article/8768e21642d94aad8c0616d20249b60e2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09373-0https://doaj.org/toc/2045-2322Abstract Little is known about the evolution, adaptation and pathogenesis of Burkholderia pseudomallei within host during acute melioidosis infection. Melioidosis is a potential life threatening disease contracted through inhalation, ingestion, inoculation or direct entry of the organism into the blood stream via wounds or skin abrasions from contaminated soil and water. Environmental B. pseudomallei strain (Bp MARAN ), isolated during a melioidosis outbreak in Pahang, Malaysia was injected intra-peritoneally into a mouse and passaged strain was recovered from spleen (Bpmouse-adapted). A gel-based comparative proteomics profiling approach was used, to map and identify differentially expressed proteins (fold-change ≥ 2; p-value ≤ 0.05) between the strains. A total of 730 and 685 spots were visualised in the Bp MARAN and Bpmouse-adapted strains, respectively. Of the 730 spots (Bp MARAN as reference gel), 87 spots were differentially regulated (44 up- and 43 down-regulated). The identified proteins were classified as proteins related to metabolism, stress response, virulence, signal transduction, or adhesion. In comparison, it was found that those proteins related to adhesins, virulence factors and stress- response were up-regulated and could possibly explain the adaptation of the bacteria in the host. Investigating the differentially expressed proteins may provide better perspective of bacterial factors which aid survivability of B. pseudomallei in host.Vanitha MariappanKumutha Malar VellasamyJamuna VadiveluNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vanitha Mariappan Kumutha Malar Vellasamy Jamuna Vadivelu Host-Adaptation of Burkholderia pseudomallei Alters Metabolism and Virulence: a Global Proteome Analysis
description	Abstract Little is known about the evolution, adaptation and pathogenesis of Burkholderia pseudomallei within host during acute melioidosis infection. Melioidosis is a potential life threatening disease contracted through inhalation, ingestion, inoculation or direct entry of the organism into the blood stream via wounds or skin abrasions from contaminated soil and water. Environmental B. pseudomallei strain (Bp MARAN ), isolated during a melioidosis outbreak in Pahang, Malaysia was injected intra-peritoneally into a mouse and passaged strain was recovered from spleen (Bpmouse-adapted). A gel-based comparative proteomics profiling approach was used, to map and identify differentially expressed proteins (fold-change ≥ 2; p-value ≤ 0.05) between the strains. A total of 730 and 685 spots were visualised in the Bp MARAN and Bpmouse-adapted strains, respectively. Of the 730 spots (Bp MARAN as reference gel), 87 spots were differentially regulated (44 up- and 43 down-regulated). The identified proteins were classified as proteins related to metabolism, stress response, virulence, signal transduction, or adhesion. In comparison, it was found that those proteins related to adhesins, virulence factors and stress- response were up-regulated and could possibly explain the adaptation of the bacteria in the host. Investigating the differentially expressed proteins may provide better perspective of bacterial factors which aid survivability of B. pseudomallei in host.
format	article
author	Vanitha Mariappan Kumutha Malar Vellasamy Jamuna Vadivelu
author_facet	Vanitha Mariappan Kumutha Malar Vellasamy Jamuna Vadivelu
author_sort	Vanitha Mariappan
title	Host-Adaptation of Burkholderia pseudomallei Alters Metabolism and Virulence: a Global Proteome Analysis
title_short	Host-Adaptation of Burkholderia pseudomallei Alters Metabolism and Virulence: a Global Proteome Analysis
title_full	Host-Adaptation of Burkholderia pseudomallei Alters Metabolism and Virulence: a Global Proteome Analysis
title_fullStr	Host-Adaptation of Burkholderia pseudomallei Alters Metabolism and Virulence: a Global Proteome Analysis
title_full_unstemmed	Host-Adaptation of Burkholderia pseudomallei Alters Metabolism and Virulence: a Global Proteome Analysis
title_sort	host-adaptation of burkholderia pseudomallei alters metabolism and virulence: a global proteome analysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8768e21642d94aad8c0616d20249b60e
work_keys_str_mv	AT vanithamariappan hostadaptationofburkholderiapseudomalleialtersmetabolismandvirulenceaglobalproteomeanalysis AT kumuthamalarvellasamy hostadaptationofburkholderiapseudomalleialtersmetabolismandvirulenceaglobalproteomeanalysis AT jamunavadivelu hostadaptationofburkholderiapseudomalleialtersmetabolismandvirulenceaglobalproteomeanalysis
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Host-Adaptation of Burkholderia pseudomallei Alters Metabolism and Virulence: a Global Proteome Analysis

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