Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.

Stratified epithelia such as the epidermis require coordinated regulation of stem and progenitor cell proliferation, survival, and differentiation to maintain homeostasis. Integrin-mediated anchorage of the basal layer stem cells of the epidermis to the underlying dermis through extracellular matrix...

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Autores principales:	Jingting Li, Yifang Chen, Manisha Tiwari, Varun Bansal, George L Sen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/876ca94cb1f14c4c935fb4a5da2f2309
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spelling	oai:doaj.org-article:876ca94cb1f14c4c935fb4a5da2f23092021-12-02T19:54:35ZRegulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.1544-91731545-788510.1371/journal.pbio.3001378https://doaj.org/article/876ca94cb1f14c4c935fb4a5da2f23092021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.3001378https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Stratified epithelia such as the epidermis require coordinated regulation of stem and progenitor cell proliferation, survival, and differentiation to maintain homeostasis. Integrin-mediated anchorage of the basal layer stem cells of the epidermis to the underlying dermis through extracellular matrix (ECM) proteins is crucial for this process. It is currently unknown how the expression of these integrins and ECM genes are regulated. Here, we show that the RNA-binding protein (RBP) heterogeneous nuclear ribonucleoprotein L (HNRNPL) binds to these genes on chromatin to promote their expression. HNRNPL recruits RNA polymerase II (Pol II) to integrin/ECM genes and is required for stabilizing Pol II transcription through those genes. In the absence of HNRNPL, the basal layer of the epidermis where the stem cells reside prematurely differentiates and detaches from the underlying dermis due to diminished integrin/ECM expression. Our results demonstrate a critical role for RBPs on chromatin to maintain stem and progenitor cell fate by dictating the expression of specific classes of genes.Jingting LiYifang ChenManisha TiwariVarun BansalGeorge L SenPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 19, Iss 9, p e3001378 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Jingting Li Yifang Chen Manisha Tiwari Varun Bansal George L Sen Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.
description	Stratified epithelia such as the epidermis require coordinated regulation of stem and progenitor cell proliferation, survival, and differentiation to maintain homeostasis. Integrin-mediated anchorage of the basal layer stem cells of the epidermis to the underlying dermis through extracellular matrix (ECM) proteins is crucial for this process. It is currently unknown how the expression of these integrins and ECM genes are regulated. Here, we show that the RNA-binding protein (RBP) heterogeneous nuclear ribonucleoprotein L (HNRNPL) binds to these genes on chromatin to promote their expression. HNRNPL recruits RNA polymerase II (Pol II) to integrin/ECM genes and is required for stabilizing Pol II transcription through those genes. In the absence of HNRNPL, the basal layer of the epidermis where the stem cells reside prematurely differentiates and detaches from the underlying dermis due to diminished integrin/ECM expression. Our results demonstrate a critical role for RBPs on chromatin to maintain stem and progenitor cell fate by dictating the expression of specific classes of genes.
format	article
author	Jingting Li Yifang Chen Manisha Tiwari Varun Bansal George L Sen
author_facet	Jingting Li Yifang Chen Manisha Tiwari Varun Bansal George L Sen
author_sort	Jingting Li
title	Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.
title_short	Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.
title_full	Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.
title_fullStr	Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.
title_full_unstemmed	Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.
title_sort	regulation of integrin and extracellular matrix genes by hnrnpl is necessary for epidermal renewal.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/876ca94cb1f14c4c935fb4a5da2f2309
work_keys_str_mv	AT jingtingli regulationofintegrinandextracellularmatrixgenesbyhnrnplisnecessaryforepidermalrenewal AT yifangchen regulationofintegrinandextracellularmatrixgenesbyhnrnplisnecessaryforepidermalrenewal AT manishatiwari regulationofintegrinandextracellularmatrixgenesbyhnrnplisnecessaryforepidermalrenewal AT varunbansal regulationofintegrinandextracellularmatrixgenesbyhnrnplisnecessaryforepidermalrenewal AT georgelsen regulationofintegrinandextracellularmatrixgenesbyhnrnplisnecessaryforepidermalrenewal
_version_	1718375912013037568

Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.

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