The distinct spatiotemporal distribution and effect of feed restriction on mtDNA copy number in broilers

Abstract Mitochondrial DNA (mtDNA) copy number reflects the abundance of mitochondria in cells and is dependent on the energy requirements of tissues. We hypothesized that the mtDNA copy number in poultry may change with age and tissue, and feed restriction may affect the growth and health of poultr...

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Autores principales:	Xiangli Zhang, Ting Wang, Jiefei Ji, Huanjie Wang, Xinghao Zhu, Pengfei Du, Yao Zhu, Yanqun Huang, Wen Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/87726ea2191d4a2abdad27231cdd3e58
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spelling	oai:doaj.org-article:87726ea2191d4a2abdad27231cdd3e582021-12-02T14:28:22ZThe distinct spatiotemporal distribution and effect of feed restriction on mtDNA copy number in broilers10.1038/s41598-020-60123-12045-2322https://doaj.org/article/87726ea2191d4a2abdad27231cdd3e582020-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-60123-1https://doaj.org/toc/2045-2322Abstract Mitochondrial DNA (mtDNA) copy number reflects the abundance of mitochondria in cells and is dependent on the energy requirements of tissues. We hypothesized that the mtDNA copy number in poultry may change with age and tissue, and feed restriction may affect the growth and health of poultry by changing mtDNA content in a tissue-specific pattern. TaqMan real-time PCR was used to quantify mtDNA copy number using three different segments of the mitochondrial genome (D-loop, ATP6, and ND6) relative to the nuclear single-copy preproglucagon gene (GCG). The effect of sex, age, and dietary restriction (quantitative, energy, and protein restriction) on mtDNA copy number variation in the tissues of broilers was investigated. We found that mtDNA copy number varied among tissues (P < 0.01) and presented a distinct change in spatiotemporal pattern. After hatching, the number of mtDNA copies significantly decreased with age in the liver and increased in muscle tissues, including heart, pectoralis, and leg muscles. Newborn broilers (unfed) and embryos (E 11 and E 17) had similar mtDNA contents in muscle tissues. Among 42 d broilers, females had a higher mtDNA copy number than males in the tissues examined. Feed restriction (8–21 d) significantly reduced the body weight but did not significantly change the mtDNA copy number of 21 d broilers. After three weeks of compensatory growth (22–42 d), only the body weight of broilers with a quantitatively restricted diet remained significantly lower than that of broilers in the control group (P < 0.05), while any type of early feed restriction significantly reduced the mtDNA copy number in muscle tissues of 42 d broilers. In summary, the mtDNA copy number of broilers was regulated in a tissue- and age-specific manner. A similar pattern of spatiotemporal change in response to early feed restriction was found in the mtDNA content of muscle tissues, including cardiac and skeletal muscle, whereas liver mtDNA content changed differently with age and dietary restriction. It seems that early restrictions in feed could effectively lower the mtDNA content in muscle cells to reduce the tissue overload in broilers at 42 d to some degree.Xiangli ZhangTing WangJiefei JiHuanjie WangXinghao ZhuPengfei DuYao ZhuYanqun HuangWen ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiangli Zhang Ting Wang Jiefei Ji Huanjie Wang Xinghao Zhu Pengfei Du Yao Zhu Yanqun Huang Wen Chen The distinct spatiotemporal distribution and effect of feed restriction on mtDNA copy number in broilers
description	Abstract Mitochondrial DNA (mtDNA) copy number reflects the abundance of mitochondria in cells and is dependent on the energy requirements of tissues. We hypothesized that the mtDNA copy number in poultry may change with age and tissue, and feed restriction may affect the growth and health of poultry by changing mtDNA content in a tissue-specific pattern. TaqMan real-time PCR was used to quantify mtDNA copy number using three different segments of the mitochondrial genome (D-loop, ATP6, and ND6) relative to the nuclear single-copy preproglucagon gene (GCG). The effect of sex, age, and dietary restriction (quantitative, energy, and protein restriction) on mtDNA copy number variation in the tissues of broilers was investigated. We found that mtDNA copy number varied among tissues (P < 0.01) and presented a distinct change in spatiotemporal pattern. After hatching, the number of mtDNA copies significantly decreased with age in the liver and increased in muscle tissues, including heart, pectoralis, and leg muscles. Newborn broilers (unfed) and embryos (E 11 and E 17) had similar mtDNA contents in muscle tissues. Among 42 d broilers, females had a higher mtDNA copy number than males in the tissues examined. Feed restriction (8–21 d) significantly reduced the body weight but did not significantly change the mtDNA copy number of 21 d broilers. After three weeks of compensatory growth (22–42 d), only the body weight of broilers with a quantitatively restricted diet remained significantly lower than that of broilers in the control group (P < 0.05), while any type of early feed restriction significantly reduced the mtDNA copy number in muscle tissues of 42 d broilers. In summary, the mtDNA copy number of broilers was regulated in a tissue- and age-specific manner. A similar pattern of spatiotemporal change in response to early feed restriction was found in the mtDNA content of muscle tissues, including cardiac and skeletal muscle, whereas liver mtDNA content changed differently with age and dietary restriction. It seems that early restrictions in feed could effectively lower the mtDNA content in muscle cells to reduce the tissue overload in broilers at 42 d to some degree.
format	article
author	Xiangli Zhang Ting Wang Jiefei Ji Huanjie Wang Xinghao Zhu Pengfei Du Yao Zhu Yanqun Huang Wen Chen
author_facet	Xiangli Zhang Ting Wang Jiefei Ji Huanjie Wang Xinghao Zhu Pengfei Du Yao Zhu Yanqun Huang Wen Chen
author_sort	Xiangli Zhang
title	The distinct spatiotemporal distribution and effect of feed restriction on mtDNA copy number in broilers
title_short	The distinct spatiotemporal distribution and effect of feed restriction on mtDNA copy number in broilers
title_full	The distinct spatiotemporal distribution and effect of feed restriction on mtDNA copy number in broilers
title_fullStr	The distinct spatiotemporal distribution and effect of feed restriction on mtDNA copy number in broilers
title_full_unstemmed	The distinct spatiotemporal distribution and effect of feed restriction on mtDNA copy number in broilers
title_sort	distinct spatiotemporal distribution and effect of feed restriction on mtdna copy number in broilers
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/87726ea2191d4a2abdad27231cdd3e58
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The distinct spatiotemporal distribution and effect of feed restriction on mtDNA copy number in broilers

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