High-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis.

<h4>Background</h4>MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in pla...

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Autores principales:	Ekua W Brenu, Kevin J Ashton, Jana Batovska, Donald R Staines, Sonya M Marshall-Gradisnik
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/87761deeb59f48fa98a0c565bc592825
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spelling	oai:doaj.org-article:87761deeb59f48fa98a0c565bc5928252021-11-25T05:59:56ZHigh-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis.1932-620310.1371/journal.pone.0102783https://doaj.org/article/87761deeb59f48fa98a0c565bc5928252014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0102783https://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME.<h4>Results</h4>Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients.<h4>Conclusion</h4>Our study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers.Ekua W BrenuKevin J AshtonJana BatovskaDonald R StainesSonya M Marshall-GradisnikPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e102783 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ekua W Brenu Kevin J Ashton Jana Batovska Donald R Staines Sonya M Marshall-Gradisnik High-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis.
description	<h4>Background</h4>MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME.<h4>Results</h4>Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients.<h4>Conclusion</h4>Our study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers.
format	article
author	Ekua W Brenu Kevin J Ashton Jana Batovska Donald R Staines Sonya M Marshall-Gradisnik
author_facet	Ekua W Brenu Kevin J Ashton Jana Batovska Donald R Staines Sonya M Marshall-Gradisnik
author_sort	Ekua W Brenu
title	High-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis.
title_short	High-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis.
title_full	High-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis.
title_fullStr	High-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis.
title_full_unstemmed	High-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis.
title_sort	high-throughput sequencing of plasma microrna in chronic fatigue syndrome/myalgic encephalomyelitis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/87761deeb59f48fa98a0c565bc592825
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High-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis.

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