Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer

Chenhui Wang,1,2,* Jiebing Yang,1,3,* Haobo Han,3 Jiawen Chen,3 Yudi Wang,3 Quanshun Li,3 Yanbo Wang1 1Department of Urology, First Hospital of Jilin University, 2Innovative Drug Research Centre, School of Pharmacy, Chongqing University, Chongqing, 3Key Laboratory for Molecular Enzymology and Engin...

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Autores principales:	Wang C, Yang J, Han H, Chen J, Wang Y, Li Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Disulfiram Poly(lactic-co-glycolic acid) Porous microparticle Non-small cell lung cancer Anti-proliferation Anti-migration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8779a7908380464d919d77fe5602228b
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spelling	oai:doaj.org-article:8779a7908380464d919d77fe5602228b2021-12-02T01:34:50ZDisulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer1178-2013https://doaj.org/article/8779a7908380464d919d77fe5602228b2017-01-01T00:00:00Zhttps://www.dovepress.com/disulfiram-loaded-porous-plga-microparticle-for-inhibiting-the-prolife-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chenhui Wang,1,2,* Jiebing Yang,1,3,* Haobo Han,3 Jiawen Chen,3 Yudi Wang,3 Quanshun Li,3 Yanbo Wang1 1Department of Urology, First Hospital of Jilin University, 2Innovative Drug Research Centre, School of Pharmacy, Chongqing University, Chongqing, 3Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, People’s Republic of China *These authors contributed equally to this work Abstract: In this study, poly(lactic-co-glycolic acid) (PLGA) was used as a carrier to construct disulfiram-loaded porous microparticle through the emulsion solvent evaporation method, using ammonium bicarbonate as a porogen. The microparticle possessed highly porous surface, suitable aerodynamic diameter for inhalation (8.31±1.33 µm), favorable drug loading (4.09%±0.11%), and sustained release profile. The antiproliferation effect of release supernatant was detected through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay using non-small-cell lung cancer A549 as a model, with only 13.3% of cell viability observed for the release supernatant at 7 days. The antiproliferation mechanism was elucidated to be associated with the enhanced induction of cell apoptosis and cell cycle arrest at S phase through flow cytometry and Western blotting analysis. Finally, wound healing and transwell migration assay showed that they could efficiently inhibit the cell migration. These results demonstrated that disulfiram-loaded porous PLGA microparticle could achieve favorable antitumor efficiency, implying the potential of treating non-small-cell lung cancer in a pulmonary administration. Keywords: disulfiram, poly(lactic-co-glycolic acid), porous microparticle, non-small-cell lung cancer, antiproliferation, antimigrationWang CYang JHan HChen JWang YLi QWang YDove Medical PressarticleDisulfiramPoly(lactic-co-glycolic acid)Porous microparticleNon-small cell lung cancerAnti-proliferationAnti-migrationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 827-837 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Disulfiram Poly(lactic-co-glycolic acid) Porous microparticle Non-small cell lung cancer Anti-proliferation Anti-migration Medicine (General) R5-920
spellingShingle	Disulfiram Poly(lactic-co-glycolic acid) Porous microparticle Non-small cell lung cancer Anti-proliferation Anti-migration Medicine (General) R5-920 Wang C Yang J Han H Chen J Wang Y Li Q Wang Y Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer
description	Chenhui Wang,1,2,* Jiebing Yang,1,3,* Haobo Han,3 Jiawen Chen,3 Yudi Wang,3 Quanshun Li,3 Yanbo Wang1 1Department of Urology, First Hospital of Jilin University, 2Innovative Drug Research Centre, School of Pharmacy, Chongqing University, Chongqing, 3Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, People’s Republic of China *These authors contributed equally to this work Abstract: In this study, poly(lactic-co-glycolic acid) (PLGA) was used as a carrier to construct disulfiram-loaded porous microparticle through the emulsion solvent evaporation method, using ammonium bicarbonate as a porogen. The microparticle possessed highly porous surface, suitable aerodynamic diameter for inhalation (8.31±1.33 µm), favorable drug loading (4.09%±0.11%), and sustained release profile. The antiproliferation effect of release supernatant was detected through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay using non-small-cell lung cancer A549 as a model, with only 13.3% of cell viability observed for the release supernatant at 7 days. The antiproliferation mechanism was elucidated to be associated with the enhanced induction of cell apoptosis and cell cycle arrest at S phase through flow cytometry and Western blotting analysis. Finally, wound healing and transwell migration assay showed that they could efficiently inhibit the cell migration. These results demonstrated that disulfiram-loaded porous PLGA microparticle could achieve favorable antitumor efficiency, implying the potential of treating non-small-cell lung cancer in a pulmonary administration. Keywords: disulfiram, poly(lactic-co-glycolic acid), porous microparticle, non-small-cell lung cancer, antiproliferation, antimigration
format	article
author	Wang C Yang J Han H Chen J Wang Y Li Q Wang Y
author_facet	Wang C Yang J Han H Chen J Wang Y Li Q Wang Y
author_sort	Wang C
title	Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer
title_short	Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer
title_full	Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer
title_fullStr	Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer
title_full_unstemmed	Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer
title_sort	disulfiram-loaded porous plga microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/8779a7908380464d919d77fe5602228b
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Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer

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