Cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization

Philip J Smith1, Maude Giroud2, Helen L Wiggins2, Florence Gower2, Jennifer A Thorley2, Bjorn Stolpe3, Julie Mazzolini2, Rosemary J Dyson4, Joshua Z Rappoport21Physical Sciences of Imaging for the Biomedical Sciences (PSIBS) Doctoral Training Center, School of Chemistry, 2School of Biosciences, 3Sch...

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Autores principales:	Smith PJ, Giroud M, Wiggins HL, Gower F, Thorley JA, Stolpe B, Mazzolini J, Dyson RJ, Rappoport JZ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/877d3b93b6324530a587ed4b441d0e8d
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spelling	oai:doaj.org-article:877d3b93b6324530a587ed4b441d0e8d2021-12-02T01:32:34ZCellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization1176-91141178-2013https://doaj.org/article/877d3b93b6324530a587ed4b441d0e8d2012-04-01T00:00:00Zhttp://www.dovepress.com/cellular-entry-of-nanoparticles-via-serum-sensitive-clathrin-mediated--a9769https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Philip J Smith1, Maude Giroud2, Helen L Wiggins2, Florence Gower2, Jennifer A Thorley2, Bjorn Stolpe3, Julie Mazzolini2, Rosemary J Dyson4, Joshua Z Rappoport21Physical Sciences of Imaging for the Biomedical Sciences (PSIBS) Doctoral Training Center, School of Chemistry, 2School of Biosciences, 3School of Geography, Earth, and Environmental Sciences, 4School of Mathematics, University of Birmingham, Edgbaston, Birmingham, United KingdomAbstract: Increasing production and application of nanomaterials raises significant questions regarding the potential for cellular entry and toxicity of nanoparticles. It was observed that the presence of serum reduces the cellular association of 20 nm carboxylate-modified fluorescent polystyrene beads up to 20-fold, relative to cells incubated in serum-free media. Analysis by confocal microscopy demonstrated that the presence of serum greatly reduces the cell surface association of nanoparticles, as well as the potential for internalization. However, both in the presence and absence of serum, nanoparticle entry depends upon clathrin-mediated endocytosis. Finally, experiments performed with cells cooled to 4&deg;C suggest that a proportion of the accumulation of nanoparticles in cells was likely due to direct permeabilization of the plasma membrane.Keywords: nanoparticles, polystyrene beads, serum, endocytosis, dynamin, clathrin, permeabilizationSmith PJGiroud MWiggins HLGower FThorley JAStolpe BMazzolini JDyson RJRappoport JZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2045-2055 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Smith PJ Giroud M Wiggins HL Gower F Thorley JA Stolpe B Mazzolini J Dyson RJ Rappoport JZ Cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization
description	Philip J Smith1, Maude Giroud2, Helen L Wiggins2, Florence Gower2, Jennifer A Thorley2, Bjorn Stolpe3, Julie Mazzolini2, Rosemary J Dyson4, Joshua Z Rappoport21Physical Sciences of Imaging for the Biomedical Sciences (PSIBS) Doctoral Training Center, School of Chemistry, 2School of Biosciences, 3School of Geography, Earth, and Environmental Sciences, 4School of Mathematics, University of Birmingham, Edgbaston, Birmingham, United KingdomAbstract: Increasing production and application of nanomaterials raises significant questions regarding the potential for cellular entry and toxicity of nanoparticles. It was observed that the presence of serum reduces the cellular association of 20 nm carboxylate-modified fluorescent polystyrene beads up to 20-fold, relative to cells incubated in serum-free media. Analysis by confocal microscopy demonstrated that the presence of serum greatly reduces the cell surface association of nanoparticles, as well as the potential for internalization. However, both in the presence and absence of serum, nanoparticle entry depends upon clathrin-mediated endocytosis. Finally, experiments performed with cells cooled to 4&deg;C suggest that a proportion of the accumulation of nanoparticles in cells was likely due to direct permeabilization of the plasma membrane.Keywords: nanoparticles, polystyrene beads, serum, endocytosis, dynamin, clathrin, permeabilization
format	article
author	Smith PJ Giroud M Wiggins HL Gower F Thorley JA Stolpe B Mazzolini J Dyson RJ Rappoport JZ
author_facet	Smith PJ Giroud M Wiggins HL Gower F Thorley JA Stolpe B Mazzolini J Dyson RJ Rappoport JZ
author_sort	Smith PJ
title	Cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization
title_short	Cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization
title_full	Cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization
title_fullStr	Cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization
title_full_unstemmed	Cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization
title_sort	cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/877d3b93b6324530a587ed4b441d0e8d
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Cellular entry of nanoparticles via serum sensitive clathrin-mediated endocytosis, and plasma membrane permeabilization

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