In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics

Rhizosphere arguably embodies the most diverse microbial ecosystem on the planet, yet it is largely a functional ‘black box’ of belowground plant-microbiome interactions. The rhizosphere is the primary site of entry for subsurface injection of fixed carbon (C) into soil with impacts on local to glob...

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Autores principales:	Richard Allen White, Joshua Rosnow, Paul D. Piehowski, Colin J. Brislawn, James J. Moran
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rhizosphere metaproteomics hot-spots metagenomics in situ non-destructive Agriculture S
Acceso en línea:	https://doaj.org/article/877e88450c9144808dffcb34ea5c4791
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id	oai:doaj.org-article:877e88450c9144808dffcb34ea5c4791
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spelling	oai:doaj.org-article:877e88450c9144808dffcb34ea5c47912021-11-25T16:08:38ZIn Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics10.3390/agronomy111122482073-4395https://doaj.org/article/877e88450c9144808dffcb34ea5c47912021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2248https://doaj.org/toc/2073-4395Rhizosphere arguably embodies the most diverse microbial ecosystem on the planet, yet it is largely a functional ‘black box’ of belowground plant-microbiome interactions. The rhizosphere is the primary site of entry for subsurface injection of fixed carbon (C) into soil with impacts on local to global scale C biogeochemistry and ultimately Earth’s climate. While spatial organization of rhizosphere is central to its function, small scale and steep microbial and geochemical gradients within this dynamic region make it easily disrupted by sampling. The significant challenge presented by sampling blocks elucidation of discreet functions, drivers, and interactions within rhizosphere ecosystems. Here, we describe a non-destructive sampling method linked to metaproteomic analysis in order to measure temporal shifts in the microbial composition and function of rhizosphere. A robust, non-destructive method of sampling microbial hotspots within rhizosphere provides an unperturbed window into the elusive functional interactome of this system over time and space.Richard Allen WhiteJoshua RosnowPaul D. PiehowskiColin J. BrislawnJames J. MoranMDPI AGarticlerhizospheremetaproteomicshot-spotsmetagenomicsin situnon-destructiveAgricultureSENAgronomy, Vol 11, Iss 2248, p 2248 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rhizosphere metaproteomics hot-spots metagenomics in situ non-destructive Agriculture S
spellingShingle	rhizosphere metaproteomics hot-spots metagenomics in situ non-destructive Agriculture S Richard Allen White Joshua Rosnow Paul D. Piehowski Colin J. Brislawn James J. Moran In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics
description	Rhizosphere arguably embodies the most diverse microbial ecosystem on the planet, yet it is largely a functional ‘black box’ of belowground plant-microbiome interactions. The rhizosphere is the primary site of entry for subsurface injection of fixed carbon (C) into soil with impacts on local to global scale C biogeochemistry and ultimately Earth’s climate. While spatial organization of rhizosphere is central to its function, small scale and steep microbial and geochemical gradients within this dynamic region make it easily disrupted by sampling. The significant challenge presented by sampling blocks elucidation of discreet functions, drivers, and interactions within rhizosphere ecosystems. Here, we describe a non-destructive sampling method linked to metaproteomic analysis in order to measure temporal shifts in the microbial composition and function of rhizosphere. A robust, non-destructive method of sampling microbial hotspots within rhizosphere provides an unperturbed window into the elusive functional interactome of this system over time and space.
format	article
author	Richard Allen White Joshua Rosnow Paul D. Piehowski Colin J. Brislawn James J. Moran
author_facet	Richard Allen White Joshua Rosnow Paul D. Piehowski Colin J. Brislawn James J. Moran
author_sort	Richard Allen White
title	In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics
title_short	In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics
title_full	In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics
title_fullStr	In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics
title_full_unstemmed	In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics
title_sort	in situ non-destructive temporal measurements of the rhizosphere microbiome ‘hot-spots’ using metaproteomics
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/877e88450c9144808dffcb34ea5c4791
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In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics

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