Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency

This work sought to address whether the presence of exogenous bicarbonate is required for pig sperm to elicit in vitro capacitation and further progesterone-induced acrosome exocytosis. For this purpose, sperm were either incubated in a standard in vitro capacitation medium or a similar medium with...

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Autores principales:	Bruna Resende Chaves, Ana Paula Pinoti Pavaneli, Olga Blanco-Prieto, Elisabeth Pinart, Sergi Bonet, Márcio Gilberto Zangeronimo, Joan E. Rodríguez-Gil, Marc Yeste
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sperm capacitation pig bicarbonate bovine serum albumin (BSA) glycogen synthase kinase (GSK3) protein kinase A (PKA) Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/87814e21f40f42fa98b6f656a772243a
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spelling	oai:doaj.org-article:87814e21f40f42fa98b6f656a772243a2021-11-25T16:47:03ZExogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency10.3390/biology101111052079-7737https://doaj.org/article/87814e21f40f42fa98b6f656a772243a2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1105https://doaj.org/toc/2079-7737This work sought to address whether the presence of exogenous bicarbonate is required for pig sperm to elicit in vitro capacitation and further progesterone-induced acrosome exocytosis. For this purpose, sperm were either incubated in a standard in vitro capacitation medium or a similar medium with different concentrations of bicarbonate (either 0 mM, 5 mM, 15 mM or 38 mM) and BSA (either 0 mg/mL or 5 mg/mL). The achievement of in vitro capacitation and progesterone-induced acrosomal exocytosis was tested through the analysis of sperm motility, plasma membrane integrity and lipid disorder, acrosome exocytosis, intracellular calcium levels, mitochondria membrane potential, O<sub>2</sub> consumption rate and the activities of both glycogen synthase kinase 3 alpha (GSK3α) and protein kinase A (PKA). While sperm incubated in media without BSA or BSA/bicarbonate, they did not achieve in vitro capacitation; those incubated in media with BSA achieved the capacitated status under any bicarbonate concentration, even when bicarbonate was absent. Moreover, there were differences related to the concentration of bicarbonate, since sperm incubated in media with BSA and with no bicarbonate or 5 mM bicarbonate showed lower overall efficiency in achieving in vitro capacitation than those incubated in the presence of BSA and 15 mM or 38 mM bicarbonate. Additionally, at the end of the experiment, sperm incubated in the presence of BSA and 38 mM bicarbonate showed significantly (<i>p</i> < 0.05) lower values of motility and plasma membrane integrity than those incubated in media with BSA and lower concentrations of bicarbonate. In conclusion, BSA is instrumental for pig sperm to elicit in vitro capacitation and trigger the subsequent progesterone-induced acrosome exocytosis. Furthermore, while exogenous bicarbonate does not seem to be essential to launch sperm capacitation, it does modulate its efficiency.Bruna Resende ChavesAna Paula Pinoti PavaneliOlga Blanco-PrietoElisabeth PinartSergi BonetMárcio Gilberto ZangeronimoJoan E. Rodríguez-GilMarc YesteMDPI AGarticlesperm capacitationpigbicarbonatebovine serum albumin (BSA)glycogen synthase kinase (GSK3)protein kinase A (PKA)Biology (General)QH301-705.5ENBiology, Vol 10, Iss 1105, p 1105 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sperm capacitation pig bicarbonate bovine serum albumin (BSA) glycogen synthase kinase (GSK3) protein kinase A (PKA) Biology (General) QH301-705.5
spellingShingle	sperm capacitation pig bicarbonate bovine serum albumin (BSA) glycogen synthase kinase (GSK3) protein kinase A (PKA) Biology (General) QH301-705.5 Bruna Resende Chaves Ana Paula Pinoti Pavaneli Olga Blanco-Prieto Elisabeth Pinart Sergi Bonet Márcio Gilberto Zangeronimo Joan E. Rodríguez-Gil Marc Yeste Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency
description	This work sought to address whether the presence of exogenous bicarbonate is required for pig sperm to elicit in vitro capacitation and further progesterone-induced acrosome exocytosis. For this purpose, sperm were either incubated in a standard in vitro capacitation medium or a similar medium with different concentrations of bicarbonate (either 0 mM, 5 mM, 15 mM or 38 mM) and BSA (either 0 mg/mL or 5 mg/mL). The achievement of in vitro capacitation and progesterone-induced acrosomal exocytosis was tested through the analysis of sperm motility, plasma membrane integrity and lipid disorder, acrosome exocytosis, intracellular calcium levels, mitochondria membrane potential, O<sub>2</sub> consumption rate and the activities of both glycogen synthase kinase 3 alpha (GSK3α) and protein kinase A (PKA). While sperm incubated in media without BSA or BSA/bicarbonate, they did not achieve in vitro capacitation; those incubated in media with BSA achieved the capacitated status under any bicarbonate concentration, even when bicarbonate was absent. Moreover, there were differences related to the concentration of bicarbonate, since sperm incubated in media with BSA and with no bicarbonate or 5 mM bicarbonate showed lower overall efficiency in achieving in vitro capacitation than those incubated in the presence of BSA and 15 mM or 38 mM bicarbonate. Additionally, at the end of the experiment, sperm incubated in the presence of BSA and 38 mM bicarbonate showed significantly (<i>p</i> < 0.05) lower values of motility and plasma membrane integrity than those incubated in media with BSA and lower concentrations of bicarbonate. In conclusion, BSA is instrumental for pig sperm to elicit in vitro capacitation and trigger the subsequent progesterone-induced acrosome exocytosis. Furthermore, while exogenous bicarbonate does not seem to be essential to launch sperm capacitation, it does modulate its efficiency.
format	article
author	Bruna Resende Chaves Ana Paula Pinoti Pavaneli Olga Blanco-Prieto Elisabeth Pinart Sergi Bonet Márcio Gilberto Zangeronimo Joan E. Rodríguez-Gil Marc Yeste
author_facet	Bruna Resende Chaves Ana Paula Pinoti Pavaneli Olga Blanco-Prieto Elisabeth Pinart Sergi Bonet Márcio Gilberto Zangeronimo Joan E. Rodríguez-Gil Marc Yeste
author_sort	Bruna Resende Chaves
title	Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency
title_short	Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency
title_full	Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency
title_fullStr	Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency
title_full_unstemmed	Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency
title_sort	exogenous albumin is crucial for pig sperm to elicit in vitro capacitation whereas bicarbonate only modulates its efficiency
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/87814e21f40f42fa98b6f656a772243a
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Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency

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