Immune complement activation is attenuated by surface nanotopography

Mats Hulander1, Anders Lundgren1, Mattias Berglin1, Mattias Ohrlander2, Jukka Lausmaa3,4, Hans Elwing1 1Department of Cell and Molecular Biology/Interface Biophysics, University of Gothenburg, Medicinaregatan 9E, Gothenburg, 2Bactiguard AB, Stockholm, 3SP Technical Research Institute, Boras, 4Biomat...

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Autores principales:	Elwing H, Lausmaa J, Ohrlander M, Berglin M, Lundgren A, Hulander M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8781de21db2f4a63a277a40fb63985ee
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spelling	oai:doaj.org-article:8781de21db2f4a63a277a40fb63985ee2021-12-02T02:44:07ZImmune complement activation is attenuated by surface nanotopography1176-91141178-2013https://doaj.org/article/8781de21db2f4a63a277a40fb63985ee2011-10-01T00:00:00Zhttp://www.dovepress.com/immune-complement-activation-is-attenuated-by-surface-nanotopography-a8572https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Mats Hulander1, Anders Lundgren1, Mattias Berglin1, Mattias Ohrlander2, Jukka Lausmaa3,4, Hans Elwing1 1Department of Cell and Molecular Biology/Interface Biophysics, University of Gothenburg, Medicinaregatan 9E, Gothenburg, 2Bactiguard AB, Stockholm, 3SP Technical Research Institute, Boras, 4Biomatcell, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Abstract: The immune complement (IC) is a cell-free protein cascade system, and the first part of the innate immune system to recognize foreign objects that enter the body. Elevated activation of the system from, for example, biomaterials or medical devices can result in both local and systemic adverse effects and eventually loss of function or rejection of the biomaterial. Here, the researchers have studied the effect of surface nanotopography on the activation of the IC system. By a simple nonlithographic process, gold nanoparticles with an average size of 58 nm were immobilized on a smooth gold substrate, creating surfaces where a nanostructure is introduced without changing the surface chemistry. The activation of the IC on smooth and nanostructured surfaces was viewed with fluorescence microscopy and quantified with quartz crystal microbalance with dissipation monitoring in human serum. Additionally, the ability of pre-adsorbed human immunoglobulin G (IgG) (a potent activator of the IC) to activate the IC after a change in surface hydrophobicity was studied. It was found that the activation of the IC was significantly attenuated on nanostructured surfaces with nearly a 50% reduction, even after pre-adsorption with IgG. An increase in surface hydrophobicity blunted this effect. The possible role of the curvature of the nanoparticles for the orientation of adsorbed IgG molecules, and how this can affect the subsequent activation of the IC, are discussed. The present findings are important for further understanding of how surface nanotopography affects complex protein adsorption, and for the future development of biomaterials and blood-contacting devices. Keywords: nanostructure, protein adsorption, gold nanoparticles, QCM-D, IgG, innate immunityElwing HLausmaa JOhrlander MBerglin MLundgren AHulander MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2653-2666 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Elwing H Lausmaa J Ohrlander M Berglin M Lundgren A Hulander M Immune complement activation is attenuated by surface nanotopography
description	Mats Hulander1, Anders Lundgren1, Mattias Berglin1, Mattias Ohrlander2, Jukka Lausmaa3,4, Hans Elwing1 1Department of Cell and Molecular Biology/Interface Biophysics, University of Gothenburg, Medicinaregatan 9E, Gothenburg, 2Bactiguard AB, Stockholm, 3SP Technical Research Institute, Boras, 4Biomatcell, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden Abstract: The immune complement (IC) is a cell-free protein cascade system, and the first part of the innate immune system to recognize foreign objects that enter the body. Elevated activation of the system from, for example, biomaterials or medical devices can result in both local and systemic adverse effects and eventually loss of function or rejection of the biomaterial. Here, the researchers have studied the effect of surface nanotopography on the activation of the IC system. By a simple nonlithographic process, gold nanoparticles with an average size of 58 nm were immobilized on a smooth gold substrate, creating surfaces where a nanostructure is introduced without changing the surface chemistry. The activation of the IC on smooth and nanostructured surfaces was viewed with fluorescence microscopy and quantified with quartz crystal microbalance with dissipation monitoring in human serum. Additionally, the ability of pre-adsorbed human immunoglobulin G (IgG) (a potent activator of the IC) to activate the IC after a change in surface hydrophobicity was studied. It was found that the activation of the IC was significantly attenuated on nanostructured surfaces with nearly a 50% reduction, even after pre-adsorption with IgG. An increase in surface hydrophobicity blunted this effect. The possible role of the curvature of the nanoparticles for the orientation of adsorbed IgG molecules, and how this can affect the subsequent activation of the IC, are discussed. The present findings are important for further understanding of how surface nanotopography affects complex protein adsorption, and for the future development of biomaterials and blood-contacting devices. Keywords: nanostructure, protein adsorption, gold nanoparticles, QCM-D, IgG, innate immunity
format	article
author	Elwing H Lausmaa J Ohrlander M Berglin M Lundgren A Hulander M
author_facet	Elwing H Lausmaa J Ohrlander M Berglin M Lundgren A Hulander M
author_sort	Elwing H
title	Immune complement activation is attenuated by surface nanotopography
title_short	Immune complement activation is attenuated by surface nanotopography
title_full	Immune complement activation is attenuated by surface nanotopography
title_fullStr	Immune complement activation is attenuated by surface nanotopography
title_full_unstemmed	Immune complement activation is attenuated by surface nanotopography
title_sort	immune complement activation is attenuated by surface nanotopography
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/8781de21db2f4a63a277a40fb63985ee
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Immune complement activation is attenuated by surface nanotopography

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