Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit

Abstract Plant-based human vaccines have been actively developed in recent years, and rice (Oryza sativa L.) is one of the best candidate crops for their production and delivery. By expressing a modified cholera toxin B (CTB) subunit, we previously developed MucoRice-CTB, a rice-based vaccine agains...

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Autores principales:	Takumi Ogawa, Koji Kashima, Yoshikazu Yuki, Mio Mejima, Shiho Kurokawa, Masaharu Kuroda, Atsushi Okazawa, Hiroshi Kiyono, Daisaku Ohta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/878a8d4f773e44a287735a995f7e344f
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spelling	oai:doaj.org-article:878a8d4f773e44a287735a995f7e344f2021-12-02T16:06:49ZSeed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit10.1038/s41598-017-04701-w2045-2322https://doaj.org/article/878a8d4f773e44a287735a995f7e344f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04701-whttps://doaj.org/toc/2045-2322Abstract Plant-based human vaccines have been actively developed in recent years, and rice (Oryza sativa L.) is one of the best candidate crops for their production and delivery. By expressing a modified cholera toxin B (CTB) subunit, we previously developed MucoRice-CTB, a rice-based vaccine against cholera, which is caused by infection of the intestine with the bacteria Vibrio cholerae. MucoRice-CTB lines have been extensively characterized by whole-genome sequencing and proteome analyses to evaluate the mutation profiles and proteome status, respectively. Here, we report non-targeted metabolomic profiling of the MucoRice-CTB transgenic rice line 51A (MR-CTB51A), MucoRice-RNAi (MR-RNAi), and their non-transgenic parent line by using gas chromatography–time-of-flight mass spectrometry. The levels of several amino acids, organic acids, carbohydrates, lipids, and secondary metabolites were significantly increased in MR-CTB51A compared with the non-transgenic parent line. These metabolomics results complement essential information obtained by genome sequencing and proteomics approaches, thereby contributing to comprehensive understanding of the properties of MucoRice-CTB as a plant-based vaccine.Takumi OgawaKoji KashimaYoshikazu YukiMio MejimaShiho KurokawaMasaharu KurodaAtsushi OkazawaHiroshi KiyonoDaisaku OhtaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takumi Ogawa Koji Kashima Yoshikazu Yuki Mio Mejima Shiho Kurokawa Masaharu Kuroda Atsushi Okazawa Hiroshi Kiyono Daisaku Ohta Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit
description	Abstract Plant-based human vaccines have been actively developed in recent years, and rice (Oryza sativa L.) is one of the best candidate crops for their production and delivery. By expressing a modified cholera toxin B (CTB) subunit, we previously developed MucoRice-CTB, a rice-based vaccine against cholera, which is caused by infection of the intestine with the bacteria Vibrio cholerae. MucoRice-CTB lines have been extensively characterized by whole-genome sequencing and proteome analyses to evaluate the mutation profiles and proteome status, respectively. Here, we report non-targeted metabolomic profiling of the MucoRice-CTB transgenic rice line 51A (MR-CTB51A), MucoRice-RNAi (MR-RNAi), and their non-transgenic parent line by using gas chromatography–time-of-flight mass spectrometry. The levels of several amino acids, organic acids, carbohydrates, lipids, and secondary metabolites were significantly increased in MR-CTB51A compared with the non-transgenic parent line. These metabolomics results complement essential information obtained by genome sequencing and proteomics approaches, thereby contributing to comprehensive understanding of the properties of MucoRice-CTB as a plant-based vaccine.
format	article
author	Takumi Ogawa Koji Kashima Yoshikazu Yuki Mio Mejima Shiho Kurokawa Masaharu Kuroda Atsushi Okazawa Hiroshi Kiyono Daisaku Ohta
author_facet	Takumi Ogawa Koji Kashima Yoshikazu Yuki Mio Mejima Shiho Kurokawa Masaharu Kuroda Atsushi Okazawa Hiroshi Kiyono Daisaku Ohta
author_sort	Takumi Ogawa
title	Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit
title_short	Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit
title_full	Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit
title_fullStr	Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit
title_full_unstemmed	Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit
title_sort	seed metabolome analysis of a transgenic rice line expressing cholera toxin b-subunit
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/878a8d4f773e44a287735a995f7e344f
work_keys_str_mv	AT takumiogawa seedmetabolomeanalysisofatransgenicricelineexpressingcholeratoxinbsubunit AT kojikashima seedmetabolomeanalysisofatransgenicricelineexpressingcholeratoxinbsubunit AT yoshikazuyuki seedmetabolomeanalysisofatransgenicricelineexpressingcholeratoxinbsubunit AT miomejima seedmetabolomeanalysisofatransgenicricelineexpressingcholeratoxinbsubunit AT shihokurokawa seedmetabolomeanalysisofatransgenicricelineexpressingcholeratoxinbsubunit AT masaharukuroda seedmetabolomeanalysisofatransgenicricelineexpressingcholeratoxinbsubunit AT atsushiokazawa seedmetabolomeanalysisofatransgenicricelineexpressingcholeratoxinbsubunit AT hiroshikiyono seedmetabolomeanalysisofatransgenicricelineexpressingcholeratoxinbsubunit AT daisakuohta seedmetabolomeanalysisofatransgenicricelineexpressingcholeratoxinbsubunit
_version_	1718384852325105664

Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit

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