Bpifcl modulates kiss2 expression under the influence of 11-ketotestosterone in female zebrafish

Abstract The bactericidal/permeability-increasing (BPI) fold-containing (BPIF) superfamily of genes expressed in the brain are purportedly involved in modulating brain function in response to stress, such as inflammation. Kisspeptin, encoded by kiss, is affected by inflammation in the brain; therefo...

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Autores principales:	Shogo Moriya, Nabila Tahsin, Ishwar S. Parhar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/879b7c8f799b43628df534ed0c845ecf
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spelling	oai:doaj.org-article:879b7c8f799b43628df534ed0c845ecf2021-12-02T11:40:44ZBpifcl modulates kiss2 expression under the influence of 11-ketotestosterone in female zebrafish10.1038/s41598-017-08248-82045-2322https://doaj.org/article/879b7c8f799b43628df534ed0c845ecf2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08248-8https://doaj.org/toc/2045-2322Abstract The bactericidal/permeability-increasing (BPI) fold-containing (BPIF) superfamily of genes expressed in the brain are purportedly involved in modulating brain function in response to stress, such as inflammation. Kisspeptin, encoded by kiss, is affected by inflammation in the brain; therefore, BPIF family genes might be involved in the modulation of kisspeptin in the brain. In this study, we investigated the expression of BPIF family C, like (bpifcl) in zebrafish brain and its involvement in kiss2 regulation. The identified, full-length sequence of a bpifcl isoform expressed in the zebrafish brain contained the BPI fold shared by BPIF family members. bpifcl mRNA expression in female zebrafish brains was significantly higher than that in males. Exposure of female zebrafish to 11-ketotestosterone decreased bpifcl and kiss2 mRNA expression. bpifcl knockdown by bpifcl-specific small interfering RNA administration to female zebrafish brain decreased kiss2 mRNA expression. bpifcl expression was widely distributed in the brain, including in the dorsal zone of the periventricular hypothalamus (Hd). Furthermore, bpifcl was also expressed in KISS2 neurons in the Hd. These results suggest that the Bpifcl modulates kiss2 mRNA expression under the influence of testosterone in the Hd of female zebrafish.Shogo MoriyaNabila TahsinIshwar S. ParharNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shogo Moriya Nabila Tahsin Ishwar S. Parhar Bpifcl modulates kiss2 expression under the influence of 11-ketotestosterone in female zebrafish
description	Abstract The bactericidal/permeability-increasing (BPI) fold-containing (BPIF) superfamily of genes expressed in the brain are purportedly involved in modulating brain function in response to stress, such as inflammation. Kisspeptin, encoded by kiss, is affected by inflammation in the brain; therefore, BPIF family genes might be involved in the modulation of kisspeptin in the brain. In this study, we investigated the expression of BPIF family C, like (bpifcl) in zebrafish brain and its involvement in kiss2 regulation. The identified, full-length sequence of a bpifcl isoform expressed in the zebrafish brain contained the BPI fold shared by BPIF family members. bpifcl mRNA expression in female zebrafish brains was significantly higher than that in males. Exposure of female zebrafish to 11-ketotestosterone decreased bpifcl and kiss2 mRNA expression. bpifcl knockdown by bpifcl-specific small interfering RNA administration to female zebrafish brain decreased kiss2 mRNA expression. bpifcl expression was widely distributed in the brain, including in the dorsal zone of the periventricular hypothalamus (Hd). Furthermore, bpifcl was also expressed in KISS2 neurons in the Hd. These results suggest that the Bpifcl modulates kiss2 mRNA expression under the influence of testosterone in the Hd of female zebrafish.
format	article
author	Shogo Moriya Nabila Tahsin Ishwar S. Parhar
author_facet	Shogo Moriya Nabila Tahsin Ishwar S. Parhar
author_sort	Shogo Moriya
title	Bpifcl modulates kiss2 expression under the influence of 11-ketotestosterone in female zebrafish
title_short	Bpifcl modulates kiss2 expression under the influence of 11-ketotestosterone in female zebrafish
title_full	Bpifcl modulates kiss2 expression under the influence of 11-ketotestosterone in female zebrafish
title_fullStr	Bpifcl modulates kiss2 expression under the influence of 11-ketotestosterone in female zebrafish
title_full_unstemmed	Bpifcl modulates kiss2 expression under the influence of 11-ketotestosterone in female zebrafish
title_sort	bpifcl modulates kiss2 expression under the influence of 11-ketotestosterone in female zebrafish
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/879b7c8f799b43628df534ed0c845ecf
work_keys_str_mv	AT shogomoriya bpifclmodulateskiss2expressionundertheinfluenceof11ketotestosteroneinfemalezebrafish AT nabilatahsin bpifclmodulateskiss2expressionundertheinfluenceof11ketotestosteroneinfemalezebrafish AT ishwarsparhar bpifclmodulateskiss2expressionundertheinfluenceof11ketotestosteroneinfemalezebrafish
_version_	1718395592372125696

Bpifcl modulates kiss2 expression under the influence of 11-ketotestosterone in female zebrafish

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