Development of an indirect competitive ELISA based on immunomagnetic beads’ clean-up for detection of maduramicin in three chicken tissues

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on immunomagnetic beads' (IMBs) clean-up was developed for detection of the residues of maduramicin (MD) in different chicken tissues. IMBs coated with a specific monoclonal antibody (MAb) against MD named MAb 2D6 were a...

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Autores principales:	Meirong Song, Zhiming Xiao, Yanan Xue, Xiya Zhang, Shuangyang Ding, Jiancheng Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	chicken elisa immunomagnetic bead maduramicin residue Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/87a158aa0dab467e860d36beb0cded1f
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spelling	oai:doaj.org-article:87a158aa0dab467e860d36beb0cded1f2021-11-26T11:19:46ZDevelopment of an indirect competitive ELISA based on immunomagnetic beads’ clean-up for detection of maduramicin in three chicken tissues0954-01051465-344310.1080/09540105.2017.1418842https://doaj.org/article/87a158aa0dab467e860d36beb0cded1f2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1418842https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on immunomagnetic beads' (IMBs) clean-up was developed for detection of the residues of maduramicin (MD) in different chicken tissues. IMBs coated with a specific monoclonal antibody (MAb) against MD named MAb 2D6 were applied to enrich the residues of MD in chicken tissues. The specificities of these IMBs were well maintained and the reversibility remained at more than 73% of the original capability after being used for three times. After elution, enriched MD was detected by a conventional ic-ELISA. The limits of detection of MD were 72, 74 and 173 μg/kg in chicken muscle, skin and fat, and liver, respectively. Recoveries ranged from 80.0% to 115.8% with coefficients of variation being less than 11.3%. These results indicated that a rapid, robust clean-up of IMBs combining ELISA provides a simple, time-saving and environmentally friendly method to detect MD in chicken tissues.Meirong SongZhiming XiaoYanan XueXiya ZhangShuangyang DingJiancheng LiTaylor & Francis Grouparticlechickenelisaimmunomagnetic beadmaduramicinresidueAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 590-599 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	chicken elisa immunomagnetic bead maduramicin residue Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	chicken elisa immunomagnetic bead maduramicin residue Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Meirong Song Zhiming Xiao Yanan Xue Xiya Zhang Shuangyang Ding Jiancheng Li Development of an indirect competitive ELISA based on immunomagnetic beads’ clean-up for detection of maduramicin in three chicken tissues
description	An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on immunomagnetic beads' (IMBs) clean-up was developed for detection of the residues of maduramicin (MD) in different chicken tissues. IMBs coated with a specific monoclonal antibody (MAb) against MD named MAb 2D6 were applied to enrich the residues of MD in chicken tissues. The specificities of these IMBs were well maintained and the reversibility remained at more than 73% of the original capability after being used for three times. After elution, enriched MD was detected by a conventional ic-ELISA. The limits of detection of MD were 72, 74 and 173 μg/kg in chicken muscle, skin and fat, and liver, respectively. Recoveries ranged from 80.0% to 115.8% with coefficients of variation being less than 11.3%. These results indicated that a rapid, robust clean-up of IMBs combining ELISA provides a simple, time-saving and environmentally friendly method to detect MD in chicken tissues.
format	article
author	Meirong Song Zhiming Xiao Yanan Xue Xiya Zhang Shuangyang Ding Jiancheng Li
author_facet	Meirong Song Zhiming Xiao Yanan Xue Xiya Zhang Shuangyang Ding Jiancheng Li
author_sort	Meirong Song
title	Development of an indirect competitive ELISA based on immunomagnetic beads’ clean-up for detection of maduramicin in three chicken tissues
title_short	Development of an indirect competitive ELISA based on immunomagnetic beads’ clean-up for detection of maduramicin in three chicken tissues
title_full	Development of an indirect competitive ELISA based on immunomagnetic beads’ clean-up for detection of maduramicin in three chicken tissues
title_fullStr	Development of an indirect competitive ELISA based on immunomagnetic beads’ clean-up for detection of maduramicin in three chicken tissues
title_full_unstemmed	Development of an indirect competitive ELISA based on immunomagnetic beads’ clean-up for detection of maduramicin in three chicken tissues
title_sort	development of an indirect competitive elisa based on immunomagnetic beads’ clean-up for detection of maduramicin in three chicken tissues
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/87a158aa0dab467e860d36beb0cded1f
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Development of an indirect competitive ELISA based on immunomagnetic beads’ clean-up for detection of maduramicin in three chicken tissues

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