Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane

Shih-Wen Peng,1 Ching-Wen Li,2 Ing-Ming Chiu,3,4 Gou-Jen Wang1–3 1Graduate Institute of Biomedical Engineering, 2Department of Mechanical Engineering, 3PhD Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, 4Institute of Cellular and System...

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Autores principales:	Peng SW, Li CW, Chiu IM, Wang GJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	nerve guidance conduit microfibrous micro/nanostructured PLGA nerve regeneration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/87a43dabac354834b7c6f993b265f4dd
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spelling	oai:doaj.org-article:87a43dabac354834b7c6f993b265f4dd2021-12-02T06:34:32ZNerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane1178-2013https://doaj.org/article/87a43dabac354834b7c6f993b265f4dd2017-01-01T00:00:00Zhttps://www.dovepress.com/nerve-guidance-conduit-with-a-hybrid-structure-of-a-plga-microfibrous--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Shih-Wen Peng,1 Ching-Wen Li,2 Ing-Ming Chiu,3,4 Gou-Jen Wang1–3 1Graduate Institute of Biomedical Engineering, 2Department of Mechanical Engineering, 3PhD Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, 4Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli, Taiwan Abstract: Nerve repair in tissue engineering involves the precise construction of a scaffold to guide nerve cell regeneration in the desired direction. However, improvements are needed to facilitate the cell migration/growth rate of nerves in the center of a nerve conduit. In this paper, we propose a nerve guidance conduit with a hybrid structure comprising a microfibrous poly(lactic-co-glycolic acid) (PLGA) bundle wrapped in a micro/nanostructured PLGA membrane. We applied sequential fabrication processes, including photolithography, nano-electroforming, and polydimethylsiloxane casting to manufacture master molds for the repeated production of the PLGA subelements. After demolding it from the master molds, we rolled the microfibrous membrane into a bundle and then wrapped it in the micro/nanostructured membrane to form a nerve-guiding conduit. We used KT98/F1B-GFP cells to estimate the migration rate and guidance ability of the fabricated nerve conduit and found that both elements increased the migration rate 1.6-fold compared with a flat PLGA membrane. We also found that 90% of the cells in the hybrid nano/microstructured membrane grew in the direction of the designed patterns. After 3 days of culturing, the interior of the nerve conduit was filled with cells, and the microfiber bundle was also surrounded by cells. Our conduit cell culture results also demonstrate that the proposed micro/nanohybrid and microfibrous structures can retain their shapes. The proposed hybrid-structured conduit demonstrates a high capability for guiding nerve cells and promoting cell migration, and, as such, is feasible for use in clinical applications. Keywords: nerve guidance conduit, microfibrous, micro/nanostructured, PLGA, nerve regenerationPeng SWLi CWChiu IMWang GJDove Medical Pressarticlenerve guidance conduitmicrofibrousmicro/nanostructuredPLGAnerve regenerationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 421-432 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	nerve guidance conduit microfibrous micro/nanostructured PLGA nerve regeneration Medicine (General) R5-920
spellingShingle	nerve guidance conduit microfibrous micro/nanostructured PLGA nerve regeneration Medicine (General) R5-920 Peng SW Li CW Chiu IM Wang GJ Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane
description	Shih-Wen Peng,1 Ching-Wen Li,2 Ing-Ming Chiu,3,4 Gou-Jen Wang1–3 1Graduate Institute of Biomedical Engineering, 2Department of Mechanical Engineering, 3PhD Program in Tissue Engineering and Regenerative Medicine, National Chung-Hsing University, Taichung, 4Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli, Taiwan Abstract: Nerve repair in tissue engineering involves the precise construction of a scaffold to guide nerve cell regeneration in the desired direction. However, improvements are needed to facilitate the cell migration/growth rate of nerves in the center of a nerve conduit. In this paper, we propose a nerve guidance conduit with a hybrid structure comprising a microfibrous poly(lactic-co-glycolic acid) (PLGA) bundle wrapped in a micro/nanostructured PLGA membrane. We applied sequential fabrication processes, including photolithography, nano-electroforming, and polydimethylsiloxane casting to manufacture master molds for the repeated production of the PLGA subelements. After demolding it from the master molds, we rolled the microfibrous membrane into a bundle and then wrapped it in the micro/nanostructured membrane to form a nerve-guiding conduit. We used KT98/F1B-GFP cells to estimate the migration rate and guidance ability of the fabricated nerve conduit and found that both elements increased the migration rate 1.6-fold compared with a flat PLGA membrane. We also found that 90% of the cells in the hybrid nano/microstructured membrane grew in the direction of the designed patterns. After 3 days of culturing, the interior of the nerve conduit was filled with cells, and the microfiber bundle was also surrounded by cells. Our conduit cell culture results also demonstrate that the proposed micro/nanohybrid and microfibrous structures can retain their shapes. The proposed hybrid-structured conduit demonstrates a high capability for guiding nerve cells and promoting cell migration, and, as such, is feasible for use in clinical applications. Keywords: nerve guidance conduit, microfibrous, micro/nanostructured, PLGA, nerve regeneration
format	article
author	Peng SW Li CW Chiu IM Wang GJ
author_facet	Peng SW Li CW Chiu IM Wang GJ
author_sort	Peng SW
title	Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane
title_short	Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane
title_full	Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane
title_fullStr	Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane
title_full_unstemmed	Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane
title_sort	nerve guidance conduit with a hybrid structure of a plga microfibrous bundle wrapped in a micro/nanostructured membrane
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/87a43dabac354834b7c6f993b265f4dd
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Nerve guidance conduit with a hybrid structure of a PLGA microfibrous bundle wrapped in a micro/nanostructured membrane

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