Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line

Abstract Microplastic and nanoplastic particles are prevalent in the environment and are beginning to enter the living system through multiple channels. Currently, little is known about the impact of plastic nanoparticles in living organisms. In order to investigate the health impact of micro- and n...

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Autores principales:	Gomathi Mahadevan, Suresh Valiyaveettil
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/87a4d3c4124140459ab8366271245702
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spelling	oai:doaj.org-article:87a4d3c4124140459ab83662712457022021-12-02T13:48:53ZUnderstanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line10.1038/s41598-020-80708-02045-2322https://doaj.org/article/87a4d3c4124140459ab83662712457022021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80708-0https://doaj.org/toc/2045-2322Abstract Microplastic and nanoplastic particles are prevalent in the environment and are beginning to enter the living system through multiple channels. Currently, little is known about the impact of plastic nanoparticles in living organisms. In order to investigate the health impact of micro- and nanoparticles of common polymers in a systematic way, luminescent plastic nanoparticles from two common polymers, polyvinyl chloride (PVC) and poly (methyl methacrylate) (PMMA) with relatively narrow size distribution are prepared using a nanoprecipitation method. As a model system, BHK-21 cells were exposed to polymer nanoparticles to understand the mode of uptake, internalization and biochemical changes inside the cells. The cellular effects of the nanoparticles were evaluated by monitoring the changes in cell viability, cell morphology, concentrations of reactive oxygen species (ROS), adenine triphosphate (ATP) and lactate dehydrogenase at different concentrations of the nanoparticles and time of exposure. PVC and PMMA nanoparticles induced a reduction in the cell viability along with a reduction of ATP and increase of ROS concentrations in a dose- and time-dependent manner. The plastic nanoparticles are internalized into the cell via endocytosis, as confirmed by Dynasore inhibition assay and colocalization with latex beads. Our findings suggest that plastic nanoparticle internalization could perturb cellular physiology and affect cell survival under laboratory conditions.Gomathi MahadevanSuresh ValiyaveettilNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gomathi Mahadevan Suresh Valiyaveettil Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line
description	Abstract Microplastic and nanoplastic particles are prevalent in the environment and are beginning to enter the living system through multiple channels. Currently, little is known about the impact of plastic nanoparticles in living organisms. In order to investigate the health impact of micro- and nanoparticles of common polymers in a systematic way, luminescent plastic nanoparticles from two common polymers, polyvinyl chloride (PVC) and poly (methyl methacrylate) (PMMA) with relatively narrow size distribution are prepared using a nanoprecipitation method. As a model system, BHK-21 cells were exposed to polymer nanoparticles to understand the mode of uptake, internalization and biochemical changes inside the cells. The cellular effects of the nanoparticles were evaluated by monitoring the changes in cell viability, cell morphology, concentrations of reactive oxygen species (ROS), adenine triphosphate (ATP) and lactate dehydrogenase at different concentrations of the nanoparticles and time of exposure. PVC and PMMA nanoparticles induced a reduction in the cell viability along with a reduction of ATP and increase of ROS concentrations in a dose- and time-dependent manner. The plastic nanoparticles are internalized into the cell via endocytosis, as confirmed by Dynasore inhibition assay and colocalization with latex beads. Our findings suggest that plastic nanoparticle internalization could perturb cellular physiology and affect cell survival under laboratory conditions.
format	article
author	Gomathi Mahadevan Suresh Valiyaveettil
author_facet	Gomathi Mahadevan Suresh Valiyaveettil
author_sort	Gomathi Mahadevan
title	Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line
title_short	Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line
title_full	Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line
title_fullStr	Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line
title_full_unstemmed	Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line
title_sort	understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with bhk-21 cell line
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/87a4d3c4124140459ab8366271245702
work_keys_str_mv	AT gomathimahadevan understandingtheinteractionsofpolymethylmethacrylateandpolyvinylchloridenanoparticleswithbhk21cellline AT sureshvaliyaveettil understandingtheinteractionsofpolymethylmethacrylateandpolyvinylchloridenanoparticleswithbhk21cellline
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Understanding the interactions of poly(methyl methacrylate) and poly(vinyl chloride) nanoparticles with BHK-21 cell line

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