The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory

Sibling species identification is very important in the understanding of malaria epidemiology. Morphological criteria are usually used in the identification of anopheline species, but this fails when sibling or cryptic species occur. Analysis by PCR-RFLP of rDNA ITS2 is currently the most reliable a...

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Autores principales:	Ign. Joko Suyono, Jesmandt Situmorang, R.C. Hidayat Soesilohadi, Rarastoeti Pratiwi
Formato:	article
Lenguaje:	EN ID
Publicado:	The Entomological Society of Indonesia 2015
Materias:	molecular identification sibling species PCR-RFLP rDNA ITS2 secondary structure Zoology QL1-991
Acceso en línea:	https://doaj.org/article/87ac93666b644eb2ab8e1ad67ef61777
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spelling	oai:doaj.org-article:87ac93666b644eb2ab8e1ad67ef617772021-12-02T04:22:11ZThe effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory1829-77222089-025710.5994/jei.10.2.93https://doaj.org/article/87ac93666b644eb2ab8e1ad67ef617772015-09-01T00:00:00Zhttp://jurnal.pei-pusat.org/index.php/jei/article/view/59https://doaj.org/toc/1829-7722https://doaj.org/toc/2089-0257Sibling species identification is very important in the understanding of malaria epidemiology. Morphological criteria are usually used in the identification of anopheline species, but this fails when sibling or cryptic species occur. Analysis by PCR-RFLP of rDNA ITS2 is currently the most reliable and sensitive method for distinguishing between members of the<em> Anopheles punctulatus</em> group. The objective of this study was to investigate the effect of DMSO concentration on ITS2 amplification of <em>An. farauti</em> from the colony maintained at BATAN Jakarta using PCR-RFLP based on the rDNA ITS2. The results showed that the addition of 6 % and 7 % DMSO produced ITS2 amplification products in the size 750 bp. DMSO could be used in PCR to relieve secondary structures when amplifying high GC templates. Molecular identification of <em>An. farauti</em> is found to be <em>Anopheles farauti</em> sensu stricto.Ign. Joko SuyonoJesmandt SitumorangR.C. Hidayat SoesilohadiRarastoeti PratiwiThe Entomological Society of Indonesiaarticlemolecular identificationsibling speciesPCR-RFLPrDNA ITS2secondary structureZoologyQL1-991ENIDJurnal Entomologi Indonesia, Vol 10, Iss 2 (2015)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	molecular identification sibling species PCR-RFLP rDNA ITS2 secondary structure Zoology QL1-991
spellingShingle	molecular identification sibling species PCR-RFLP rDNA ITS2 secondary structure Zoology QL1-991 Ign. Joko Suyono Jesmandt Situmorang R.C. Hidayat Soesilohadi Rarastoeti Pratiwi The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory
description	Sibling species identification is very important in the understanding of malaria epidemiology. Morphological criteria are usually used in the identification of anopheline species, but this fails when sibling or cryptic species occur. Analysis by PCR-RFLP of rDNA ITS2 is currently the most reliable and sensitive method for distinguishing between members of the<em> Anopheles punctulatus</em> group. The objective of this study was to investigate the effect of DMSO concentration on ITS2 amplification of <em>An. farauti</em> from the colony maintained at BATAN Jakarta using PCR-RFLP based on the rDNA ITS2. The results showed that the addition of 6 % and 7 % DMSO produced ITS2 amplification products in the size 750 bp. DMSO could be used in PCR to relieve secondary structures when amplifying high GC templates. Molecular identification of <em>An. farauti</em> is found to be <em>Anopheles farauti</em> sensu stricto.
format	article
author	Ign. Joko Suyono Jesmandt Situmorang R.C. Hidayat Soesilohadi Rarastoeti Pratiwi
author_facet	Ign. Joko Suyono Jesmandt Situmorang R.C. Hidayat Soesilohadi Rarastoeti Pratiwi
author_sort	Ign. Joko Suyono
title	The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory
title_short	The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory
title_full	The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory
title_fullStr	The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory
title_full_unstemmed	The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory
title_sort	effect of dmso on its2 amplification in the molecular identification of anopheles farauti laveran (diptera: culicidae), from a colony established in the laboratory
publisher	The Entomological Society of Indonesia
publishDate	2015
url	https://doaj.org/article/87ac93666b644eb2ab8e1ad67ef61777
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The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory

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