(-)-Epigallocatechin-3-gallate Ameliorates Intervertebral Disc Degeneration Through Reprogramming of the Circadian Clock

The circadian clock is vital in the management of our daily physiological as well as metabolic processes. Disturbances of the clock can cause degenerative and age-related diseases. Increasing evidence has indicated that the intervertebral discs contain an internal biological clock related to degener...

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Autores principales:	Liangwei Mei, Yi Zheng, Teng Ma, Bing Xia, Xue Gao, Yiming Hao, Zhuojing Luo, Jinghui Huang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	circadian clock intervertebral disc degeneration (IDD) (-)-epigallocatechin-3-gallate (EGCG) oxidative stress Bmal1 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/87ade570b5f64563875bdde52b7fd042
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spelling	oai:doaj.org-article:87ade570b5f64563875bdde52b7fd0422021-11-04T05:55:59Z(-)-Epigallocatechin-3-gallate Ameliorates Intervertebral Disc Degeneration Through Reprogramming of the Circadian Clock1663-981210.3389/fphar.2021.753548https://doaj.org/article/87ade570b5f64563875bdde52b7fd0422021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.753548/fullhttps://doaj.org/toc/1663-9812The circadian clock is vital in the management of our daily physiological as well as metabolic processes. Disturbances of the clock can cause degenerative and age-related diseases. Increasing evidence has indicated that the intervertebral discs contain an internal biological clock related to degeneration. However, to date, no bioactive compounds have been found that can ameliorate intervertebral disc degeneration (IDD) by restoring the circadian clock. (-)-Epigallocatechin-3-gallate (EGCG) is a nutritious food with powerful antioxidant properties, as well as entraining biological clock to improve health. The purpose of this study was to determine whether the protective effects of EGCG on nucleus pulposus (NPCs) under oxidative stress is related to the circadian clock. First, we found that EGCG attenuated H2O2-induced extracellular matrix degradation in NPCs and inhibited H2O2-induced NPC apoptosis. Our in vivo experiments also confirmed this finding. Furthermore, EGCG attenuated H2O2-triggered dampening of phase shifts and daily oscillations in circadian clock gene transcription as well as protein expression levels. Intriguingly, core clock gene (Bmal1) knockdown notably blocked the protective effects of EGCG. To our knowledge, this study provides the first convincing evidence that EGCG prevents IDD in a Bmal1-dependent manner. In general, EGCG supplementation can be used as a nutritional prevention strategy for the rehabilitation of degenerative diseases related to the circadian clock.Liangwei MeiYi ZhengTeng MaBing XiaXue GaoYiming HaoZhuojing LuoJinghui HuangFrontiers Media S.A.articlecircadian clockintervertebral disc degeneration (IDD)(-)-epigallocatechin-3-gallate (EGCG)oxidative stressBmal1Therapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circadian clock intervertebral disc degeneration (IDD) (-)-epigallocatechin-3-gallate (EGCG) oxidative stress Bmal1 Therapeutics. Pharmacology RM1-950
spellingShingle	circadian clock intervertebral disc degeneration (IDD) (-)-epigallocatechin-3-gallate (EGCG) oxidative stress Bmal1 Therapeutics. Pharmacology RM1-950 Liangwei Mei Yi Zheng Teng Ma Bing Xia Xue Gao Yiming Hao Zhuojing Luo Jinghui Huang (-)-Epigallocatechin-3-gallate Ameliorates Intervertebral Disc Degeneration Through Reprogramming of the Circadian Clock
description	The circadian clock is vital in the management of our daily physiological as well as metabolic processes. Disturbances of the clock can cause degenerative and age-related diseases. Increasing evidence has indicated that the intervertebral discs contain an internal biological clock related to degeneration. However, to date, no bioactive compounds have been found that can ameliorate intervertebral disc degeneration (IDD) by restoring the circadian clock. (-)-Epigallocatechin-3-gallate (EGCG) is a nutritious food with powerful antioxidant properties, as well as entraining biological clock to improve health. The purpose of this study was to determine whether the protective effects of EGCG on nucleus pulposus (NPCs) under oxidative stress is related to the circadian clock. First, we found that EGCG attenuated H2O2-induced extracellular matrix degradation in NPCs and inhibited H2O2-induced NPC apoptosis. Our in vivo experiments also confirmed this finding. Furthermore, EGCG attenuated H2O2-triggered dampening of phase shifts and daily oscillations in circadian clock gene transcription as well as protein expression levels. Intriguingly, core clock gene (Bmal1) knockdown notably blocked the protective effects of EGCG. To our knowledge, this study provides the first convincing evidence that EGCG prevents IDD in a Bmal1-dependent manner. In general, EGCG supplementation can be used as a nutritional prevention strategy for the rehabilitation of degenerative diseases related to the circadian clock.
format	article
author	Liangwei Mei Yi Zheng Teng Ma Bing Xia Xue Gao Yiming Hao Zhuojing Luo Jinghui Huang
author_facet	Liangwei Mei Yi Zheng Teng Ma Bing Xia Xue Gao Yiming Hao Zhuojing Luo Jinghui Huang
author_sort	Liangwei Mei
title	(-)-Epigallocatechin-3-gallate Ameliorates Intervertebral Disc Degeneration Through Reprogramming of the Circadian Clock
title_short	(-)-Epigallocatechin-3-gallate Ameliorates Intervertebral Disc Degeneration Through Reprogramming of the Circadian Clock
title_full	(-)-Epigallocatechin-3-gallate Ameliorates Intervertebral Disc Degeneration Through Reprogramming of the Circadian Clock
title_fullStr	(-)-Epigallocatechin-3-gallate Ameliorates Intervertebral Disc Degeneration Through Reprogramming of the Circadian Clock
title_full_unstemmed	(-)-Epigallocatechin-3-gallate Ameliorates Intervertebral Disc Degeneration Through Reprogramming of the Circadian Clock
title_sort	(-)-epigallocatechin-3-gallate ameliorates intervertebral disc degeneration through reprogramming of the circadian clock
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/87ade570b5f64563875bdde52b7fd042
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(-)-Epigallocatechin-3-gallate Ameliorates Intervertebral Disc Degeneration Through Reprogramming of the Circadian Clock

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