Contribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia
Coagulase is a critical factor for distinguishing <i>Staphylococcus aureus</i> and coagulase-negative Staphylococcus. Our previous studies demonstrated that the null mutation of coagulase (<i>coa</i>) or its direct regulator, SaeRS, significantly enhanced the ability of S<...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/87af1dc9661140668e0a11eec5174c96 |
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| Sumario: | Coagulase is a critical factor for distinguishing <i>Staphylococcus aureus</i> and coagulase-negative Staphylococcus. Our previous studies demonstrated that the null mutation of coagulase (<i>coa</i>) or its direct regulator, SaeRS, significantly enhanced the ability of S<i>. aureus</i> (CA-MRSA 923) to survive in human blood in vitro. This led us to further investigate the role of coagulase and its direct regulator, SaeRS, in the pathogenicity of CA-MRSA 923 in bacteremia during infection. In this study, we found that the null mutation of <i>coa</i> significantly decreased the mortality of CA-MRSA 923; moreover, the single null mutation of <i>saeRS</i> and the double deletion of <i>coa</i>/<i>saeRS</i> abolished the virulence of CA-MRSA 923. Moreover, the mice infected with either the <i>saeRS</i> knockout or the <i>coa</i>/<i>saeRS</i> double knockout mutant exhibited fewer histological lesions and less neutrophils infiltration in the infected kidneys compared to those infected with the <i>coa</i> knockout mutant or their parental control. Furthermore, we examined the impact of <i>coa</i> and <i>saeRS</i> on bacterial survival in vitro. The null mutation of <i>coa</i> had no impact on bacterial survival in mice blood, whereas the deletion mutation of <i>saeRS</i> or <i>coa</i>/<i>saeRS</i> significantly enhanced bacterial survival in mice blood. These data indicate that SaeRS plays a key role in the lethality of CA-MRSA 923 bacteremia, and that coagulase is one of the important virulence factors that is regulated by SaeRS and contributes to the pathogenicity of CA–MRSA 923. |
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