Contribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia

Coagulase is a critical factor for distinguishing <i>Staphylococcus aureus</i> and coagulase-negative Staphylococcus. Our previous studies demonstrated that the null mutation of coagulase (<i>coa</i>) or its direct regulator, SaeRS, significantly enhanced the ability of S<...

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Autores principales:	Ying Liu, Wei Gao, Junshu Yang, Haiyong Guo, Jiang Zhang, Yinduo Ji
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Staphylococcus aureus</i> CA-MRSA coagulase SaeRS regulator virulence bacteremia Medicine R
Acceso en línea:	https://doaj.org/article/87af1dc9661140668e0a11eec5174c96
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spelling	oai:doaj.org-article:87af1dc9661140668e0a11eec5174c962021-11-25T18:37:54ZContribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia10.3390/pathogens101113962076-0817https://doaj.org/article/87af1dc9661140668e0a11eec5174c962021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1396https://doaj.org/toc/2076-0817Coagulase is a critical factor for distinguishing <i>Staphylococcus aureus</i> and coagulase-negative Staphylococcus. Our previous studies demonstrated that the null mutation of coagulase (<i>coa</i>) or its direct regulator, SaeRS, significantly enhanced the ability of S<i>. aureus</i> (CA-MRSA 923) to survive in human blood in vitro. This led us to further investigate the role of coagulase and its direct regulator, SaeRS, in the pathogenicity of CA-MRSA 923 in bacteremia during infection. In this study, we found that the null mutation of <i>coa</i> significantly decreased the mortality of CA-MRSA 923; moreover, the single null mutation of <i>saeRS</i> and the double deletion of <i>coa</i>/<i>saeRS</i> abolished the virulence of CA-MRSA 923. Moreover, the mice infected with either the <i>saeRS</i> knockout or the <i>coa</i>/<i>saeRS</i> double knockout mutant exhibited fewer histological lesions and less neutrophils infiltration in the infected kidneys compared to those infected with the <i>coa</i> knockout mutant or their parental control. Furthermore, we examined the impact of <i>coa</i> and <i>saeRS</i> on bacterial survival in vitro. The null mutation of <i>coa</i> had no impact on bacterial survival in mice blood, whereas the deletion mutation of <i>saeRS</i> or <i>coa</i>/<i>saeRS</i> significantly enhanced bacterial survival in mice blood. These data indicate that SaeRS plays a key role in the lethality of CA-MRSA 923 bacteremia, and that coagulase is one of the important virulence factors that is regulated by SaeRS and contributes to the pathogenicity of CA–MRSA 923.Ying LiuWei GaoJunshu YangHaiyong GuoJiang ZhangYinduo JiMDPI AGarticle<i>Staphylococcus aureus</i>CA-MRSAcoagulaseSaeRS regulatorvirulencebacteremiaMedicineRENPathogens, Vol 10, Iss 1396, p 1396 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Staphylococcus aureus</i> CA-MRSA coagulase SaeRS regulator virulence bacteremia Medicine R
spellingShingle	<i>Staphylococcus aureus</i> CA-MRSA coagulase SaeRS regulator virulence bacteremia Medicine R Ying Liu Wei Gao Junshu Yang Haiyong Guo Jiang Zhang Yinduo Ji Contribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia
description	Coagulase is a critical factor for distinguishing <i>Staphylococcus aureus</i> and coagulase-negative Staphylococcus. Our previous studies demonstrated that the null mutation of coagulase (<i>coa</i>) or its direct regulator, SaeRS, significantly enhanced the ability of S<i>. aureus</i> (CA-MRSA 923) to survive in human blood in vitro. This led us to further investigate the role of coagulase and its direct regulator, SaeRS, in the pathogenicity of CA-MRSA 923 in bacteremia during infection. In this study, we found that the null mutation of <i>coa</i> significantly decreased the mortality of CA-MRSA 923; moreover, the single null mutation of <i>saeRS</i> and the double deletion of <i>coa</i>/<i>saeRS</i> abolished the virulence of CA-MRSA 923. Moreover, the mice infected with either the <i>saeRS</i> knockout or the <i>coa</i>/<i>saeRS</i> double knockout mutant exhibited fewer histological lesions and less neutrophils infiltration in the infected kidneys compared to those infected with the <i>coa</i> knockout mutant or their parental control. Furthermore, we examined the impact of <i>coa</i> and <i>saeRS</i> on bacterial survival in vitro. The null mutation of <i>coa</i> had no impact on bacterial survival in mice blood, whereas the deletion mutation of <i>saeRS</i> or <i>coa</i>/<i>saeRS</i> significantly enhanced bacterial survival in mice blood. These data indicate that SaeRS plays a key role in the lethality of CA-MRSA 923 bacteremia, and that coagulase is one of the important virulence factors that is regulated by SaeRS and contributes to the pathogenicity of CA–MRSA 923.
format	article
author	Ying Liu Wei Gao Junshu Yang Haiyong Guo Jiang Zhang Yinduo Ji
author_facet	Ying Liu Wei Gao Junshu Yang Haiyong Guo Jiang Zhang Yinduo Ji
author_sort	Ying Liu
title	Contribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia
title_short	Contribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia
title_full	Contribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia
title_fullStr	Contribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia
title_full_unstemmed	Contribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia
title_sort	contribution of coagulase and its regulator saers to lethality of ca-mrsa 923 bacteremia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/87af1dc9661140668e0a11eec5174c96
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Contribution of Coagulase and Its Regulator SaeRS to Lethality of CA-MRSA 923 Bacteremia

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