A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS

Abstract. Present study was performed to investigate the influence of polysaccharide fragment or lipid A upon induction of TNFα and IL-6 cytokines. The study was performed with human THP-1 monocytic leukemia cells that were induced to differentiate into macrophage-like cells using PMA treatment. Bac...

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Autores principales:	E. V. Voloshina, S. V. Zubova, S. V. Prokhorenko, N. I. Kosjakova, I. R. Prokhorenko
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	lipopolysaccharide thp-1 cells tnfα il-6 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/87af23ca3b494916a568744bc47f730b
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spelling	oai:doaj.org-article:87af23ca3b494916a568744bc47f730b2021-11-18T08:03:40ZA COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS1563-06252313-741X10.15789/1563-0625-2009-6-509-514https://doaj.org/article/87af23ca3b494916a568744bc47f730b2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/351https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Present study was performed to investigate the influence of polysaccharide fragment or lipid A upon induction of TNFα and IL-6 cytokines. The study was performed with human THP-1 monocytic leukemia cells that were induced to differentiate into macrophage-like cells using PMA treatment. Bacterial lipopolysaccharides from S. typhimurium (S-chemotype form), S. typhimurium SL1181 (R-chemotype, Re-mutant), E. coli O55:B5 (S-chemotype), and E. coli JM103 (R-chemotype, Re-mutant) were used in this study. A decreased molar ratio for lipid A-KDO in S-form of LPS from E. coli is accompanied by diminished TNFα and IL-6 expression. By the contrast, for S-form of LPS from Salmonella, a decrease in lipid A-KDO molar ratio did cause a sufficient enhancement of TNFα expression. A contribution of lipid A structure into biological activity of LPS is more significant for Re-chemotype than for S-chemotype, independently on bacterial species.E. V. VoloshinaS. V. ZubovaS. V. ProkhorenkoN. I. KosjakovaI. R. ProkhorenkoSPb RAACIarticlelipopolysaccharidethp-1 cellstnfαil-6Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 11, Iss 6, Pp 509-514 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	lipopolysaccharide thp-1 cells tnfα il-6 Immunologic diseases. Allergy RC581-607
spellingShingle	lipopolysaccharide thp-1 cells tnfα il-6 Immunologic diseases. Allergy RC581-607 E. V. Voloshina S. V. Zubova S. V. Prokhorenko N. I. Kosjakova I. R. Prokhorenko A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS
description	Abstract. Present study was performed to investigate the influence of polysaccharide fragment or lipid A upon induction of TNFα and IL-6 cytokines. The study was performed with human THP-1 monocytic leukemia cells that were induced to differentiate into macrophage-like cells using PMA treatment. Bacterial lipopolysaccharides from S. typhimurium (S-chemotype form), S. typhimurium SL1181 (R-chemotype, Re-mutant), E. coli O55:B5 (S-chemotype), and E. coli JM103 (R-chemotype, Re-mutant) were used in this study. A decreased molar ratio for lipid A-KDO in S-form of LPS from E. coli is accompanied by diminished TNFα and IL-6 expression. By the contrast, for S-form of LPS from Salmonella, a decrease in lipid A-KDO molar ratio did cause a sufficient enhancement of TNFα expression. A contribution of lipid A structure into biological activity of LPS is more significant for Re-chemotype than for S-chemotype, independently on bacterial species.
format	article
author	E. V. Voloshina S. V. Zubova S. V. Prokhorenko N. I. Kosjakova I. R. Prokhorenko
author_facet	E. V. Voloshina S. V. Zubova S. V. Prokhorenko N. I. Kosjakova I. R. Prokhorenko
author_sort	E. V. Voloshina
title	A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS
title_short	A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS
title_full	A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS
title_fullStr	A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS
title_full_unstemmed	A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS
title_sort	comparison of different lipopolysaccharide chemotypes from escherichia coli and salmonella upon synthesis of tnfα and il-6 by macrophage-like thp-1 cells
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/87af23ca3b494916a568744bc47f730b
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A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS

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