The Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts

Phytopathogenic fungi infect crops, presenting a worldwide threat to agriculture. Polyene macrolides are one of the most effective antifungal agents applied in human therapy and crop protection. In this study, we found a cryptic polyene biosynthetic gene cluster in <i>Actinokineospora sphecios...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ying Tang, Cuiyang Zhang, Tianqi Cui, Ping Lei, Zhaohui Guo, Hailong Wang, Qingshu Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polyene macrolide actinospene genome mining <i>Actinokineospora spheciospongiae</i> antifungal activity cryptic gene cluster Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/87b71c72d34243659f90b09f1bb49d01
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:87b71c72d34243659f90b09f1bb49d01
record_format	dspace
spelling	oai:doaj.org-article:87b71c72d34243659f90b09f1bb49d012021-11-25T18:29:15ZThe Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts10.3390/molecules262270201420-3049https://doaj.org/article/87b71c72d34243659f90b09f1bb49d012021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/7020https://doaj.org/toc/1420-3049Phytopathogenic fungi infect crops, presenting a worldwide threat to agriculture. Polyene macrolides are one of the most effective antifungal agents applied in human therapy and crop protection. In this study, we found a cryptic polyene biosynthetic gene cluster in <i>Actinokineospora spheciospongiae</i> by genome mining. Then, this gene cluster was activated via varying fermentation conditions, leading to the discovery of new polyene actinospene (<b>1</b>), which was subsequently isolated and its structure determined through spectroscopic techniques including UV, HR-MS, and NMR. The absolute configuration was confirmed by comparing the calculated and experimental electronic circular dichroism (ECD) spectra. Unlike known polyene macrolides, actinospene (<b>1</b>) demonstrated more versatile post-assembling decorations including two epoxide groups and an unusual isobutenyl side chain. In bioassays, actinospene (<b>1</b>) showed a broad spectrum of antifungal activity against several plant fungal pathogens as well as pathogenic yeasts with minimum inhibitory concentrations ranging between 2 and 10 μg/mL.Ying TangCuiyang ZhangTianqi CuiPing LeiZhaohui GuoHailong WangQingshu LiuMDPI AGarticlepolyene macrolideactinospenegenome mining<i>Actinokineospora spheciospongiae</i>antifungal activitycryptic gene clusterOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 7020, p 7020 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyene macrolide actinospene genome mining <i>Actinokineospora spheciospongiae</i> antifungal activity cryptic gene cluster Organic chemistry QD241-441
spellingShingle	polyene macrolide actinospene genome mining <i>Actinokineospora spheciospongiae</i> antifungal activity cryptic gene cluster Organic chemistry QD241-441 Ying Tang Cuiyang Zhang Tianqi Cui Ping Lei Zhaohui Guo Hailong Wang Qingshu Liu The Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts
description	Phytopathogenic fungi infect crops, presenting a worldwide threat to agriculture. Polyene macrolides are one of the most effective antifungal agents applied in human therapy and crop protection. In this study, we found a cryptic polyene biosynthetic gene cluster in <i>Actinokineospora spheciospongiae</i> by genome mining. Then, this gene cluster was activated via varying fermentation conditions, leading to the discovery of new polyene actinospene (<b>1</b>), which was subsequently isolated and its structure determined through spectroscopic techniques including UV, HR-MS, and NMR. The absolute configuration was confirmed by comparing the calculated and experimental electronic circular dichroism (ECD) spectra. Unlike known polyene macrolides, actinospene (<b>1</b>) demonstrated more versatile post-assembling decorations including two epoxide groups and an unusual isobutenyl side chain. In bioassays, actinospene (<b>1</b>) showed a broad spectrum of antifungal activity against several plant fungal pathogens as well as pathogenic yeasts with minimum inhibitory concentrations ranging between 2 and 10 μg/mL.
format	article
author	Ying Tang Cuiyang Zhang Tianqi Cui Ping Lei Zhaohui Guo Hailong Wang Qingshu Liu
author_facet	Ying Tang Cuiyang Zhang Tianqi Cui Ping Lei Zhaohui Guo Hailong Wang Qingshu Liu
author_sort	Ying Tang
title	The Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts
title_short	The Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts
title_full	The Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts
title_fullStr	The Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts
title_full_unstemmed	The Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts
title_sort	discovery of actinospene, a new polyene macrolide with broad activity against plant fungal pathogens and pathogenic yeasts
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/87b71c72d34243659f90b09f1bb49d01
work_keys_str_mv	AT yingtang thediscoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT cuiyangzhang thediscoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT tianqicui thediscoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT pinglei thediscoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT zhaohuiguo thediscoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT hailongwang thediscoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT qingshuliu thediscoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT yingtang discoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT cuiyangzhang discoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT tianqicui discoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT pinglei discoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT zhaohuiguo discoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT hailongwang discoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts AT qingshuliu discoveryofactinospeneanewpolyenemacrolidewithbroadactivityagainstplantfungalpathogensandpathogenicyeasts
_version_	1718411088001761280

The Discovery of Actinospene, a New Polyene Macrolide with Broad Activity against Plant Fungal Pathogens and Pathogenic Yeasts

Ejemplares similares