Microfluidic active loading of single cells enables analysis of complex clinical specimens

Single-cell detection methods are limited by the trade-off between flow rate and measurement precision. Here the authors introduce active loading, an optically triggered microfluidic system to concentrate diluted cell samples, which reduces clogging and decreases processing time in single-cell assay...

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Main Authors:	Nicholas L. Calistri, Robert J. Kimmerling, Seth W. Malinowski, Mehdi Touat, Mark M. Stevens, Selim Olcum, Keith L. Ligon, Scott R. Manalis
Format:	article
Language:	EN
Published:	Nature Portfolio 2018
Subjects:	Science Q
Online Access:	https://doaj.org/article/87b9574143a84e82ab152a42f9a0ce1c
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spelling	oai:doaj.org-article:87b9574143a84e82ab152a42f9a0ce1c2021-12-02T15:34:20ZMicrofluidic active loading of single cells enables analysis of complex clinical specimens10.1038/s41467-018-07283-x2041-1723https://doaj.org/article/87b9574143a84e82ab152a42f9a0ce1c2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07283-xhttps://doaj.org/toc/2041-1723Single-cell detection methods are limited by the trade-off between flow rate and measurement precision. Here the authors introduce active loading, an optically triggered microfluidic system to concentrate diluted cell samples, which reduces clogging and decreases processing time in single-cell assays.Nicholas L. CalistriRobert J. KimmerlingSeth W. MalinowskiMehdi TouatMark M. StevensSelim OlcumKeith L. LigonScott R. ManalisNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Nicholas L. Calistri Robert J. Kimmerling Seth W. Malinowski Mehdi Touat Mark M. Stevens Selim Olcum Keith L. Ligon Scott R. Manalis Microfluidic active loading of single cells enables analysis of complex clinical specimens
description	Single-cell detection methods are limited by the trade-off between flow rate and measurement precision. Here the authors introduce active loading, an optically triggered microfluidic system to concentrate diluted cell samples, which reduces clogging and decreases processing time in single-cell assays.
format	article
author	Nicholas L. Calistri Robert J. Kimmerling Seth W. Malinowski Mehdi Touat Mark M. Stevens Selim Olcum Keith L. Ligon Scott R. Manalis
author_facet	Nicholas L. Calistri Robert J. Kimmerling Seth W. Malinowski Mehdi Touat Mark M. Stevens Selim Olcum Keith L. Ligon Scott R. Manalis
author_sort	Nicholas L. Calistri
title	Microfluidic active loading of single cells enables analysis of complex clinical specimens
title_short	Microfluidic active loading of single cells enables analysis of complex clinical specimens
title_full	Microfluidic active loading of single cells enables analysis of complex clinical specimens
title_fullStr	Microfluidic active loading of single cells enables analysis of complex clinical specimens
title_full_unstemmed	Microfluidic active loading of single cells enables analysis of complex clinical specimens
title_sort	microfluidic active loading of single cells enables analysis of complex clinical specimens
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/87b9574143a84e82ab152a42f9a0ce1c
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Microfluidic active loading of single cells enables analysis of complex clinical specimens

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