Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats

Background: Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD) that is characterized by acute and chronic inflammation.The etiology and pathophysiology of IBD is unidentified, and there are many obstacles on the definite treatment ofthis disease. Recently, the useful ef...

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Autores principales:	Nader Tanideh, Meghdad Bahrani, Mohammad J. Khoshnood-Mansoorkhani, Davood Mehrabani, Donya Firoozi, Omid Koohi-Hosseinabadi, Aida Iraji
Formato:	article
Lenguaje:	EN
Publicado:	Shiraz University of Medical Sciences 2016
Materias:	inflammatory bowel disease ulcerative colitis antioxidant melillotus officinalis l Medicine R
Acceso en línea:	https://doaj.org/article/87bf404bee9f41a195f67345315dcc1d
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spelling	oai:doaj.org-article:87bf404bee9f41a195f67345315dcc1d2021-11-15T09:50:13ZEvaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats2783-2430https://doaj.org/article/87bf404bee9f41a195f67345315dcc1d2016-12-01T00:00:00Zhttps://colorectalresearch.sums.ac.ir/article_47150_58f6d6730c9a6beaffdbf37405704076.pdfhttps://doaj.org/toc/2783-2430Background: Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD) that is characterized by acute and chronic inflammation.The etiology and pathophysiology of IBD is unidentified, and there are many obstacles on the definite treatment ofthis disease. Recently, the useful effects of some herbal medicine on improving UC have been studied. Melillotus officinalis L. (M.officinalis) is an herb with antioxidant and anti-inflammatory effects used as food, forage and medicine.Objectives: This study evaluated the antioxidant effects of M. officinalis aqueous extracts in the acetic acid- induced ulcerative colitisin rats.Methods: Fifty rats were randomly divided into five equal groups. Group I (Control healthy group) received 1 mL/kg of normalsaline orally. Group II (control colitis group) received 1 mL/kg of normal saline orally. Group III (positive control) received 3 mg/kgprednisolone orally. Group IV received 1000 mg/kg M. officinalis aqueous extracts orally. Group V received 2000 mg/kg M. officinalisaqueous extracts orally. Ulcerative colitis was induced by intra-rectal acetic acid (3% v/v) administration. All treatments were done24 hours after induction of colitis and continued for seven days. On the eighth day, the rats were sacrificed and colonic biopsieswere taken for histopathological and biochemical studies. Data analysis was performed, using SPSS software and significance levelwas set at P≤0.05.Results: Treatment with M. officinalis aqueous extract could enhance colonic antioxidant capacity and decrease inflammation andacute colonic injury induced by acetic acid, which is dose-dependent. In addition, administrating the extract significantly (P≤0.05)reduced the colonic level of malondialdehyde and myeloperoxidase, and significantly (P ≤ 0.05) increased the level of reducedglutathione (P≤0.05). The extract had more effects at the dose of 2000 mg/kg than 1000 mg/kg dosage and prednisolone.Conclusions: This study revealed that M. officinalis had apparent curative effects on treating UC because of its antioxidant and antiinflammatoryactivities.Nader TanidehMeghdad BahraniMohammad J. Khoshnood-MansoorkhaniDavood MehrabaniDonya FirooziOmid Koohi-HosseinabadiAida IrajiShiraz University of Medical Sciencesarticleinflammatory bowel diseaseulcerative colitisantioxidantmelillotus officinalis lMedicineRENIranian Journal of Colorectal Research, Vol 4, Iss 4 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	inflammatory bowel disease ulcerative colitis antioxidant melillotus officinalis l Medicine R
spellingShingle	inflammatory bowel disease ulcerative colitis antioxidant melillotus officinalis l Medicine R Nader Tanideh Meghdad Bahrani Mohammad J. Khoshnood-Mansoorkhani Davood Mehrabani Donya Firoozi Omid Koohi-Hosseinabadi Aida Iraji Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats
description	Background: Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD) that is characterized by acute and chronic inflammation.The etiology and pathophysiology of IBD is unidentified, and there are many obstacles on the definite treatment ofthis disease. Recently, the useful effects of some herbal medicine on improving UC have been studied. Melillotus officinalis L. (M.officinalis) is an herb with antioxidant and anti-inflammatory effects used as food, forage and medicine.Objectives: This study evaluated the antioxidant effects of M. officinalis aqueous extracts in the acetic acid- induced ulcerative colitisin rats.Methods: Fifty rats were randomly divided into five equal groups. Group I (Control healthy group) received 1 mL/kg of normalsaline orally. Group II (control colitis group) received 1 mL/kg of normal saline orally. Group III (positive control) received 3 mg/kgprednisolone orally. Group IV received 1000 mg/kg M. officinalis aqueous extracts orally. Group V received 2000 mg/kg M. officinalisaqueous extracts orally. Ulcerative colitis was induced by intra-rectal acetic acid (3% v/v) administration. All treatments were done24 hours after induction of colitis and continued for seven days. On the eighth day, the rats were sacrificed and colonic biopsieswere taken for histopathological and biochemical studies. Data analysis was performed, using SPSS software and significance levelwas set at P≤0.05.Results: Treatment with M. officinalis aqueous extract could enhance colonic antioxidant capacity and decrease inflammation andacute colonic injury induced by acetic acid, which is dose-dependent. In addition, administrating the extract significantly (P≤0.05)reduced the colonic level of malondialdehyde and myeloperoxidase, and significantly (P ≤ 0.05) increased the level of reducedglutathione (P≤0.05). The extract had more effects at the dose of 2000 mg/kg than 1000 mg/kg dosage and prednisolone.Conclusions: This study revealed that M. officinalis had apparent curative effects on treating UC because of its antioxidant and antiinflammatoryactivities.
format	article
author	Nader Tanideh Meghdad Bahrani Mohammad J. Khoshnood-Mansoorkhani Davood Mehrabani Donya Firoozi Omid Koohi-Hosseinabadi Aida Iraji
author_facet	Nader Tanideh Meghdad Bahrani Mohammad J. Khoshnood-Mansoorkhani Davood Mehrabani Donya Firoozi Omid Koohi-Hosseinabadi Aida Iraji
author_sort	Nader Tanideh
title	Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats
title_short	Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats
title_full	Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats
title_fullStr	Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats
title_full_unstemmed	Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats
title_sort	evaluating the effect of melillotus officinalis l. aqueous extracts on healing of acetic acid-induced ulcerative colitis in male rats
publisher	Shiraz University of Medical Sciences
publishDate	2016
url	https://doaj.org/article/87bf404bee9f41a195f67345315dcc1d
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Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats

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