Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation

There is very little information on the transgenerational or genetic effects of low dose-rate ionizing radiation. We report the detection of the transgenerational effects of chronic low dose-rate irradiation in mice, at the molecular level in the whole genome, using array comparative genomic hybridi...

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Autores principales:	Keiji Ogura, Yoshiko Ayabe, Chihiro Harada, Ignacia Braga Tanaka, Satoshi Tanaka, Jun-ichiro Komura
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ionizing radiation low dose-rate transgenerational effects genetic effects copy number variation (CNV) array comprehensive genomic hybridization (array CGH) Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/87cde449a2ba4eb7b38b9026b6cdc156
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spelling	oai:doaj.org-article:87cde449a2ba4eb7b38b9026b6cdc1562021-11-25T17:56:38ZIncreased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation10.3390/ijms2222124371422-00671661-6596https://doaj.org/article/87cde449a2ba4eb7b38b9026b6cdc1562021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12437https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067There is very little information on the transgenerational or genetic effects of low dose-rate ionizing radiation. We report the detection of the transgenerational effects of chronic low dose-rate irradiation in mice, at the molecular level in the whole genome, using array comparative genomic hybridization technology. We observed that the number of the mice with de novo copy number variations (specifically, deletions) was significantly increased in the offspring of C57BL/6J male mice exposed to 20 mGy/day gamma-rays for 400 days (total dose: 8000 mGy), as compared to non-irradiated controls. We did not detect any difference in the size of the de novo deletions between the irradiated and the non-irradiated groups. An analysis of the life span of the offspring suggested a possibility that de novo copy-number variations may be associated with shorter life spans.Keiji OguraYoshiko AyabeChihiro HaradaIgnacia Braga TanakaSatoshi TanakaJun-ichiro KomuraMDPI AGarticleionizing radiationlow dose-ratetransgenerational effectsgenetic effectscopy number variation (CNV)array comprehensive genomic hybridization (array CGH)Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12437, p 12437 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ionizing radiation low dose-rate transgenerational effects genetic effects copy number variation (CNV) array comprehensive genomic hybridization (array CGH) Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	ionizing radiation low dose-rate transgenerational effects genetic effects copy number variation (CNV) array comprehensive genomic hybridization (array CGH) Biology (General) QH301-705.5 Chemistry QD1-999 Keiji Ogura Yoshiko Ayabe Chihiro Harada Ignacia Braga Tanaka Satoshi Tanaka Jun-ichiro Komura Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation
description	There is very little information on the transgenerational or genetic effects of low dose-rate ionizing radiation. We report the detection of the transgenerational effects of chronic low dose-rate irradiation in mice, at the molecular level in the whole genome, using array comparative genomic hybridization technology. We observed that the number of the mice with de novo copy number variations (specifically, deletions) was significantly increased in the offspring of C57BL/6J male mice exposed to 20 mGy/day gamma-rays for 400 days (total dose: 8000 mGy), as compared to non-irradiated controls. We did not detect any difference in the size of the de novo deletions between the irradiated and the non-irradiated groups. An analysis of the life span of the offspring suggested a possibility that de novo copy-number variations may be associated with shorter life spans.
format	article
author	Keiji Ogura Yoshiko Ayabe Chihiro Harada Ignacia Braga Tanaka Satoshi Tanaka Jun-ichiro Komura
author_facet	Keiji Ogura Yoshiko Ayabe Chihiro Harada Ignacia Braga Tanaka Satoshi Tanaka Jun-ichiro Komura
author_sort	Keiji Ogura
title	Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation
title_short	Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation
title_full	Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation
title_fullStr	Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation
title_full_unstemmed	Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation
title_sort	increased frequency of copy number variations revealed by array comparative genomic hybridization in the offspring of male mice exposed to low dose-rate ionizing radiation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/87cde449a2ba4eb7b38b9026b6cdc156
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Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation

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