Probing Multicellular Tissue Fusion of Cocultured Spheroids—A 3D‐Bioassembly Model

Abstract While decades of research have enriched the knowledge of how to grow cells into mature tissues, little is yet known about the next phase: fusing of these engineered tissues into larger functional structures. The specific effect of multicellular interfaces on tissue fusion remains largely un...

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Autores principales:	Gabriella C. J. Lindberg, Xiaolin Cui, Mitchell Durham, Laura Veenendaal, Benjamin S. Schon, Gary J. Hooper, Khoon S. Lim, Tim B. F. Woodfield
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	3D‐bioassembly cartilage tissues cocultured spheroids high throughput microtissues spheroid fusion Science Q
Acceso en línea:	https://doaj.org/article/87d9dbf986684de386293a43cd8fa3b3
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spelling	oai:doaj.org-article:87d9dbf986684de386293a43cd8fa3b32021-11-17T08:40:31ZProbing Multicellular Tissue Fusion of Cocultured Spheroids—A 3D‐Bioassembly Model2198-384410.1002/advs.202103320https://doaj.org/article/87d9dbf986684de386293a43cd8fa3b32021-11-01T00:00:00Zhttps://doi.org/10.1002/advs.202103320https://doaj.org/toc/2198-3844Abstract While decades of research have enriched the knowledge of how to grow cells into mature tissues, little is yet known about the next phase: fusing of these engineered tissues into larger functional structures. The specific effect of multicellular interfaces on tissue fusion remains largely unexplored. Here, a facile 3D‐bioassembly platform is introduced to primarily study fusion of cartilage–cartilage interfaces using spheroids formed from human mesenchymal stromal cells (hMSCs) and articular chondrocytes (hACs). 3D‐bioassembly of two adjacent hMSCs spheroids displays coordinated migration and noteworthy matrix deposition while the interface between two hAC tissues lacks both cells and type‐II collagen. Cocultures contribute to increased phenotypic stability in the fusion region while close initial contact between hMSCs and hACs (mixed) yields superior hyaline differentiation over more distant, indirect cocultures. This reduced ability of potent hMSCs to fuse with mature hAC tissue further underlines the major clinical challenge that is integration. Together, this data offer the first proof of an in vitro 3D‐model to reliably study lateral fusion mechanisms between multicellular spheroids and mature cartilage tissues. Ultimately, this high‐throughput 3D‐bioassembly model provides a bridge between understanding cellular differentiation and tissue fusion and offers the potential to probe fundamental biological mechanisms that underpin organogenesis.Gabriella C. J. LindbergXiaolin CuiMitchell DurhamLaura VeenendaalBenjamin S. SchonGary J. HooperKhoon S. LimTim B. F. WoodfieldWileyarticle3D‐bioassemblycartilage tissuescocultured spheroidshigh throughputmicrotissuesspheroid fusionScienceQENAdvanced Science, Vol 8, Iss 22, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	3D‐bioassembly cartilage tissues cocultured spheroids high throughput microtissues spheroid fusion Science Q
spellingShingle	3D‐bioassembly cartilage tissues cocultured spheroids high throughput microtissues spheroid fusion Science Q Gabriella C. J. Lindberg Xiaolin Cui Mitchell Durham Laura Veenendaal Benjamin S. Schon Gary J. Hooper Khoon S. Lim Tim B. F. Woodfield Probing Multicellular Tissue Fusion of Cocultured Spheroids—A 3D‐Bioassembly Model
description	Abstract While decades of research have enriched the knowledge of how to grow cells into mature tissues, little is yet known about the next phase: fusing of these engineered tissues into larger functional structures. The specific effect of multicellular interfaces on tissue fusion remains largely unexplored. Here, a facile 3D‐bioassembly platform is introduced to primarily study fusion of cartilage–cartilage interfaces using spheroids formed from human mesenchymal stromal cells (hMSCs) and articular chondrocytes (hACs). 3D‐bioassembly of two adjacent hMSCs spheroids displays coordinated migration and noteworthy matrix deposition while the interface between two hAC tissues lacks both cells and type‐II collagen. Cocultures contribute to increased phenotypic stability in the fusion region while close initial contact between hMSCs and hACs (mixed) yields superior hyaline differentiation over more distant, indirect cocultures. This reduced ability of potent hMSCs to fuse with mature hAC tissue further underlines the major clinical challenge that is integration. Together, this data offer the first proof of an in vitro 3D‐model to reliably study lateral fusion mechanisms between multicellular spheroids and mature cartilage tissues. Ultimately, this high‐throughput 3D‐bioassembly model provides a bridge between understanding cellular differentiation and tissue fusion and offers the potential to probe fundamental biological mechanisms that underpin organogenesis.
format	article
author	Gabriella C. J. Lindberg Xiaolin Cui Mitchell Durham Laura Veenendaal Benjamin S. Schon Gary J. Hooper Khoon S. Lim Tim B. F. Woodfield
author_facet	Gabriella C. J. Lindberg Xiaolin Cui Mitchell Durham Laura Veenendaal Benjamin S. Schon Gary J. Hooper Khoon S. Lim Tim B. F. Woodfield
author_sort	Gabriella C. J. Lindberg
title	Probing Multicellular Tissue Fusion of Cocultured Spheroids—A 3D‐Bioassembly Model
title_short	Probing Multicellular Tissue Fusion of Cocultured Spheroids—A 3D‐Bioassembly Model
title_full	Probing Multicellular Tissue Fusion of Cocultured Spheroids—A 3D‐Bioassembly Model
title_fullStr	Probing Multicellular Tissue Fusion of Cocultured Spheroids—A 3D‐Bioassembly Model
title_full_unstemmed	Probing Multicellular Tissue Fusion of Cocultured Spheroids—A 3D‐Bioassembly Model
title_sort	probing multicellular tissue fusion of cocultured spheroids—a 3d‐bioassembly model
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/87d9dbf986684de386293a43cd8fa3b3
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_version_	1718425682226184192

Probing Multicellular Tissue Fusion of Cocultured Spheroids—A 3D‐Bioassembly Model

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