Single virus genomics: a new tool for virus discovery.

Whole genome amplification and sequencing of single microbial cells has significantly influenced genomics and microbial ecology by facilitating direct recovery of reference genome data. However, viral genomics continues to suffer due to difficulties related to the isolation and characterization of u...

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Autores principales:	Lisa Zeigler Allen, Thomas Ishoey, Mark A Novotny, Jeffrey S McLean, Roger S Lasken, Shannon J Williamson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/87e70087d2784e6d8e93e02ad5c4b07b
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spelling	oai:doaj.org-article:87e70087d2784e6d8e93e02ad5c4b07b2021-11-18T06:56:57ZSingle virus genomics: a new tool for virus discovery.1932-620310.1371/journal.pone.0017722https://doaj.org/article/87e70087d2784e6d8e93e02ad5c4b07b2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21436882/?tool=EBIhttps://doaj.org/toc/1932-6203Whole genome amplification and sequencing of single microbial cells has significantly influenced genomics and microbial ecology by facilitating direct recovery of reference genome data. However, viral genomics continues to suffer due to difficulties related to the isolation and characterization of uncultivated viruses. We report here on a new approach called 'Single Virus Genomics', which enabled the isolation and complete genome sequencing of the first single virus particle. A mixed assemblage comprised of two known viruses; E. coli bacteriophages lambda and T4, were sorted using flow cytometric methods and subsequently immobilized in an agarose matrix. Genome amplification was then achieved in situ via multiple displacement amplification (MDA). The complete lambda phage genome was recovered with an average depth of coverage of approximately 437X. The isolation and genome sequencing of uncultivated viruses using Single Virus Genomics approaches will enable researchers to address questions about viral diversity, evolution, adaptation and ecology that were previously unattainable.Lisa Zeigler AllenThomas IshoeyMark A NovotnyJeffrey S McLeanRoger S LaskenShannon J WilliamsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17722 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lisa Zeigler Allen Thomas Ishoey Mark A Novotny Jeffrey S McLean Roger S Lasken Shannon J Williamson Single virus genomics: a new tool for virus discovery.
description	Whole genome amplification and sequencing of single microbial cells has significantly influenced genomics and microbial ecology by facilitating direct recovery of reference genome data. However, viral genomics continues to suffer due to difficulties related to the isolation and characterization of uncultivated viruses. We report here on a new approach called 'Single Virus Genomics', which enabled the isolation and complete genome sequencing of the first single virus particle. A mixed assemblage comprised of two known viruses; E. coli bacteriophages lambda and T4, were sorted using flow cytometric methods and subsequently immobilized in an agarose matrix. Genome amplification was then achieved in situ via multiple displacement amplification (MDA). The complete lambda phage genome was recovered with an average depth of coverage of approximately 437X. The isolation and genome sequencing of uncultivated viruses using Single Virus Genomics approaches will enable researchers to address questions about viral diversity, evolution, adaptation and ecology that were previously unattainable.
format	article
author	Lisa Zeigler Allen Thomas Ishoey Mark A Novotny Jeffrey S McLean Roger S Lasken Shannon J Williamson
author_facet	Lisa Zeigler Allen Thomas Ishoey Mark A Novotny Jeffrey S McLean Roger S Lasken Shannon J Williamson
author_sort	Lisa Zeigler Allen
title	Single virus genomics: a new tool for virus discovery.
title_short	Single virus genomics: a new tool for virus discovery.
title_full	Single virus genomics: a new tool for virus discovery.
title_fullStr	Single virus genomics: a new tool for virus discovery.
title_full_unstemmed	Single virus genomics: a new tool for virus discovery.
title_sort	single virus genomics: a new tool for virus discovery.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/87e70087d2784e6d8e93e02ad5c4b07b
work_keys_str_mv	AT lisazeiglerallen singlevirusgenomicsanewtoolforvirusdiscovery AT thomasishoey singlevirusgenomicsanewtoolforvirusdiscovery AT markanovotny singlevirusgenomicsanewtoolforvirusdiscovery AT jeffreysmclean singlevirusgenomicsanewtoolforvirusdiscovery AT rogerslasken singlevirusgenomicsanewtoolforvirusdiscovery AT shannonjwilliamson singlevirusgenomicsanewtoolforvirusdiscovery
_version_	1718424177990434816

Single virus genomics: a new tool for virus discovery.

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