Integrated Analysis of Mutations, miRNA and mRNA Expression in Glioblastoma

ShiChao Wang,1 HuanMin Zhou,1 RuiJian Zhang,2 YanRu Zhang1 1College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, People’s Republic of China; 2Department of Neurosurgery, The People’s Hospital of Inner Mongolia, Hohhot, Inner Mongolia, 010017, People’s Rep...

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Autores principales:	Wang S, Zhou H, Zhang R, Zhang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	glioblastoma multiforme transcriptome mirna Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/87f4ecc137aa4d4a85e1f90ac39a4e2b
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spelling	oai:doaj.org-article:87f4ecc137aa4d4a85e1f90ac39a4e2b2021-11-16T18:47:50ZIntegrated Analysis of Mutations, miRNA and mRNA Expression in Glioblastoma1178-7074https://doaj.org/article/87f4ecc137aa4d4a85e1f90ac39a4e2b2021-11-01T00:00:00Zhttps://www.dovepress.com/integrated-analysis-of-mutations-mirna-and-mrna-expression-in-glioblas-peer-reviewed-fulltext-article-IJGMhttps://doaj.org/toc/1178-7074ShiChao Wang,1 HuanMin Zhou,1 RuiJian Zhang,2 YanRu Zhang1 1College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, People’s Republic of China; 2Department of Neurosurgery, The People’s Hospital of Inner Mongolia, Hohhot, Inner Mongolia, 010017, People’s Republic of ChinaCorrespondence: YanRu Zhang Tel +976 18047183314Email mdmzhh_2008@126.comBackground: Glioblastoma multiforme (GBM) is a common, malignant brain tumor in adults, with a median survival of only 15– 23 months. Organisms respond to disease stress through sophisticated mechanisms at the physiological, transcriptional and metabolic levels. However, the molecular regulatory networks responsible for occurrence, progression and recurrence of glioma have yet to be elucidated.Methods: In this study, we sought to determine the cause of gliomas by developing an RNA-seq technique that analyzes mRNA and small RNA (sRNA) with the aim of discovering potential methods for precisely blocking key signaling pathways in occurrence, progression, and recurrence. The explication of mechanisms leading to GBM formation has become a feasible and promising new therapeutic method.Results: GBM-associated genes were identified based on their expression during the disease stress response. Analysis of the inverse correlations between microRNAs (miRNAs) and target mRNAs revealed 43 mRNA–miRNA interactions during disease progression. BOC-SMO and BOC-RAS were found to promote the malignant progression of glioma. A total of 3088 differentially expressed genes were identified as involved in several biological processes, such as amino acid metabolism, protein transport associated with immune response, cell proliferation, and cell apoptosis. Fifteen miRNAs were also identified as being differentially expressed in GBM and control groups.Conclusion: The results of this study provide an important foundation for understanding the pathogenesis of glioma and discovering new therapeutic targets.Keywords: glioblastoma multiforme, transcriptome, miRNAWang SZhou HZhang RZhang YDove Medical Pressarticleglioblastoma multiformetranscriptomemirnaMedicine (General)R5-920ENInternational Journal of General Medicine, Vol Volume 14, Pp 8281-8292 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioblastoma multiforme transcriptome mirna Medicine (General) R5-920
spellingShingle	glioblastoma multiforme transcriptome mirna Medicine (General) R5-920 Wang S Zhou H Zhang R Zhang Y Integrated Analysis of Mutations, miRNA and mRNA Expression in Glioblastoma
description	ShiChao Wang,1 HuanMin Zhou,1 RuiJian Zhang,2 YanRu Zhang1 1College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, People’s Republic of China; 2Department of Neurosurgery, The People’s Hospital of Inner Mongolia, Hohhot, Inner Mongolia, 010017, People’s Republic of ChinaCorrespondence: YanRu Zhang Tel +976 18047183314Email mdmzhh_2008@126.comBackground: Glioblastoma multiforme (GBM) is a common, malignant brain tumor in adults, with a median survival of only 15– 23 months. Organisms respond to disease stress through sophisticated mechanisms at the physiological, transcriptional and metabolic levels. However, the molecular regulatory networks responsible for occurrence, progression and recurrence of glioma have yet to be elucidated.Methods: In this study, we sought to determine the cause of gliomas by developing an RNA-seq technique that analyzes mRNA and small RNA (sRNA) with the aim of discovering potential methods for precisely blocking key signaling pathways in occurrence, progression, and recurrence. The explication of mechanisms leading to GBM formation has become a feasible and promising new therapeutic method.Results: GBM-associated genes were identified based on their expression during the disease stress response. Analysis of the inverse correlations between microRNAs (miRNAs) and target mRNAs revealed 43 mRNA–miRNA interactions during disease progression. BOC-SMO and BOC-RAS were found to promote the malignant progression of glioma. A total of 3088 differentially expressed genes were identified as involved in several biological processes, such as amino acid metabolism, protein transport associated with immune response, cell proliferation, and cell apoptosis. Fifteen miRNAs were also identified as being differentially expressed in GBM and control groups.Conclusion: The results of this study provide an important foundation for understanding the pathogenesis of glioma and discovering new therapeutic targets.Keywords: glioblastoma multiforme, transcriptome, miRNA
format	article
author	Wang S Zhou H Zhang R Zhang Y
author_facet	Wang S Zhou H Zhang R Zhang Y
author_sort	Wang S
title	Integrated Analysis of Mutations, miRNA and mRNA Expression in Glioblastoma
title_short	Integrated Analysis of Mutations, miRNA and mRNA Expression in Glioblastoma
title_full	Integrated Analysis of Mutations, miRNA and mRNA Expression in Glioblastoma
title_fullStr	Integrated Analysis of Mutations, miRNA and mRNA Expression in Glioblastoma
title_full_unstemmed	Integrated Analysis of Mutations, miRNA and mRNA Expression in Glioblastoma
title_sort	integrated analysis of mutations, mirna and mrna expression in glioblastoma
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/87f4ecc137aa4d4a85e1f90ac39a4e2b
work_keys_str_mv	AT wangs integratedanalysisofmutationsmirnaandmrnaexpressioninglioblastoma AT zhouh integratedanalysisofmutationsmirnaandmrnaexpressioninglioblastoma AT zhangr integratedanalysisofmutationsmirnaandmrnaexpressioninglioblastoma AT zhangy integratedanalysisofmutationsmirnaandmrnaexpressioninglioblastoma
_version_	1718426252218466304

Integrated Analysis of Mutations, miRNA and mRNA Expression in Glioblastoma

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