Magnesium-doped Nanostructured Titanium Surface Modulates Macrophage-mediated Inflammatory Response for Ameliorative Osseointegration

Xinrui Qiao, Jie Yang, Yuli Shang, Shu Deng, Shiyu Yao, Zhe Wang, Yi Guo, Cheng Peng Department of Stomatology, The Second Hospital of Tianjin Medical University, Tianjin, People’s Republic of ChinaCorrespondence: Cheng Peng Department of StomotologyThe Second Hospital of Tianjin Medical U...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qiao X, Yang J, Shang Y, Deng S, Yao S, Wang Z, Guo Y, Peng C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	tio2 nanotube，magnesium ions，osteoimmunomodulation，macrophage Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/87f79eaf2c0f4b6eb149e95f00122201
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:87f79eaf2c0f4b6eb149e95f00122201
record_format	dspace
spelling	oai:doaj.org-article:87f79eaf2c0f4b6eb149e95f001222012021-12-02T08:59:44ZMagnesium-doped Nanostructured Titanium Surface Modulates Macrophage-mediated Inflammatory Response for Ameliorative Osseointegration1178-2013https://doaj.org/article/87f79eaf2c0f4b6eb149e95f001222012020-09-01T00:00:00Zhttps://www.dovepress.com/magnesium-doped-nanostructured-titanium-surface-modulates-macrophage-m-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xinrui Qiao, Jie Yang, Yuli Shang, Shu Deng, Shiyu Yao, Zhe Wang, Yi Guo, Cheng Peng Department of Stomatology, The Second Hospital of Tianjin Medical University, Tianjin, People’s Republic of ChinaCorrespondence: Cheng Peng Department of StomotologyThe Second Hospital of Tianjin Medical University, 23 Pingjiang Road, Hexi District, Tianjin 300211, People’s Republic of ChinaTel +86 139 2007 9346Email peng_cheng2013@163.comBackground: Next generation of coating materials on the surface of implants is designed with a paradigm shift from an inert material to an osteoimmunomodulatory material. Regulating immune response to biomedical implants through influencing the polarization of macrophage has been proven to be an effective strategy.Methods: Through anodization and hydrothermal treatment, magnesium ion incorporated TiO2 nanotube array (MgN) coating was fabricated on the surface of titanium and it is hypothesized that it has osteoimmunomodulatory properties. To verify this assumption, systematic studies were carried out by in vitro and in vivo experiments.Results: Mg ion release behavior results showed that MgN coating was successfully fabricated on the surface of titanium using anodization and hydrothermal technology. Scanning electron microscopy (SEM) images showed the morphology of the MgN coating on the titanium. The expression of inflammation-related genes (IL-6, IL-1β, TNF-α) was downregulated in MgN group compared with TiO2 nanotube (NT) and blank Ti groups, but anti-inflammatory genes (IL-10 and IL-1ra) were remarkably upregulated in the MgN group. The in vitro and in vivo results demonstrated that MgN coating influenced macrophage polarization toward the M2 phenotype compared with NT and blank-Ti groups, which enhanced osteogenic differentiation of rat bone mesenchymal stem cells rBMSCs in conditioned media (CM) generated by macrophages.Conclusion: MgN coating on the titanium endowed the surface with immune-regulatory features and exerted an advantageous effect on osteogenesis, thereby providing excellent strategies for the surface modification of biomedical implants.Keywords: TiO2 nanotube, magnesium ions, osteoimmunomodulation, macrophageQiao XYang JShang YDeng SYao SWang ZGuo YPeng CDove Medical Pressarticletio2 nanotube，magnesium ions，osteoimmunomodulation，macrophageMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 7185-7198 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	tio2 nanotube，magnesium ions，osteoimmunomodulation，macrophage Medicine (General) R5-920
spellingShingle	tio2 nanotube，magnesium ions，osteoimmunomodulation，macrophage Medicine (General) R5-920 Qiao X Yang J Shang Y Deng S Yao S Wang Z Guo Y Peng C Magnesium-doped Nanostructured Titanium Surface Modulates Macrophage-mediated Inflammatory Response for Ameliorative Osseointegration
description	Xinrui Qiao, Jie Yang, Yuli Shang, Shu Deng, Shiyu Yao, Zhe Wang, Yi Guo, Cheng Peng Department of Stomatology, The Second Hospital of Tianjin Medical University, Tianjin, People’s Republic of ChinaCorrespondence: Cheng Peng Department of StomotologyThe Second Hospital of Tianjin Medical University, 23 Pingjiang Road, Hexi District, Tianjin 300211, People’s Republic of ChinaTel +86 139 2007 9346Email peng_cheng2013@163.comBackground: Next generation of coating materials on the surface of implants is designed with a paradigm shift from an inert material to an osteoimmunomodulatory material. Regulating immune response to biomedical implants through influencing the polarization of macrophage has been proven to be an effective strategy.Methods: Through anodization and hydrothermal treatment, magnesium ion incorporated TiO2 nanotube array (MgN) coating was fabricated on the surface of titanium and it is hypothesized that it has osteoimmunomodulatory properties. To verify this assumption, systematic studies were carried out by in vitro and in vivo experiments.Results: Mg ion release behavior results showed that MgN coating was successfully fabricated on the surface of titanium using anodization and hydrothermal technology. Scanning electron microscopy (SEM) images showed the morphology of the MgN coating on the titanium. The expression of inflammation-related genes (IL-6, IL-1β, TNF-α) was downregulated in MgN group compared with TiO2 nanotube (NT) and blank Ti groups, but anti-inflammatory genes (IL-10 and IL-1ra) were remarkably upregulated in the MgN group. The in vitro and in vivo results demonstrated that MgN coating influenced macrophage polarization toward the M2 phenotype compared with NT and blank-Ti groups, which enhanced osteogenic differentiation of rat bone mesenchymal stem cells rBMSCs in conditioned media (CM) generated by macrophages.Conclusion: MgN coating on the titanium endowed the surface with immune-regulatory features and exerted an advantageous effect on osteogenesis, thereby providing excellent strategies for the surface modification of biomedical implants.Keywords: TiO2 nanotube, magnesium ions, osteoimmunomodulation, macrophage
format	article
author	Qiao X Yang J Shang Y Deng S Yao S Wang Z Guo Y Peng C
author_facet	Qiao X Yang J Shang Y Deng S Yao S Wang Z Guo Y Peng C
author_sort	Qiao X
title	Magnesium-doped Nanostructured Titanium Surface Modulates Macrophage-mediated Inflammatory Response for Ameliorative Osseointegration
title_short	Magnesium-doped Nanostructured Titanium Surface Modulates Macrophage-mediated Inflammatory Response for Ameliorative Osseointegration
title_full	Magnesium-doped Nanostructured Titanium Surface Modulates Macrophage-mediated Inflammatory Response for Ameliorative Osseointegration
title_fullStr	Magnesium-doped Nanostructured Titanium Surface Modulates Macrophage-mediated Inflammatory Response for Ameliorative Osseointegration
title_full_unstemmed	Magnesium-doped Nanostructured Titanium Surface Modulates Macrophage-mediated Inflammatory Response for Ameliorative Osseointegration
title_sort	magnesium-doped nanostructured titanium surface modulates macrophage-mediated inflammatory response for ameliorative osseointegration
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/87f79eaf2c0f4b6eb149e95f00122201
work_keys_str_mv	AT qiaox magnesiumdopednanostructuredtitaniumsurfacemodulatesmacrophagemediatedinflammatoryresponseforameliorativeosseointegration AT yangj magnesiumdopednanostructuredtitaniumsurfacemodulatesmacrophagemediatedinflammatoryresponseforameliorativeosseointegration AT shangy magnesiumdopednanostructuredtitaniumsurfacemodulatesmacrophagemediatedinflammatoryresponseforameliorativeosseointegration AT dengs magnesiumdopednanostructuredtitaniumsurfacemodulatesmacrophagemediatedinflammatoryresponseforameliorativeosseointegration AT yaos magnesiumdopednanostructuredtitaniumsurfacemodulatesmacrophagemediatedinflammatoryresponseforameliorativeosseointegration AT wangz magnesiumdopednanostructuredtitaniumsurfacemodulatesmacrophagemediatedinflammatoryresponseforameliorativeosseointegration AT guoy magnesiumdopednanostructuredtitaniumsurfacemodulatesmacrophagemediatedinflammatoryresponseforameliorativeosseointegration AT pengc magnesiumdopednanostructuredtitaniumsurfacemodulatesmacrophagemediatedinflammatoryresponseforameliorativeosseointegration
_version_	1718398314480664576

Magnesium-doped Nanostructured Titanium Surface Modulates Macrophage-mediated Inflammatory Response for Ameliorative Osseointegration

Ejemplares similares