The MogR transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in Listeria monocytogenes.

Flagella are surface structures critical for motility and virulence of many bacterial species. In Listeria monocytogenes, MogR tightly represses expression of flagellin (FlaA) during extracellular growth at 37 degrees C and during intracellular infection. MogR is also required for full virulence in...

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Autores principales:	Aimee Shen, Darren E Higgins
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2006
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/87fea8adbb2d48459e1a3a7221f97547
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spelling	oai:doaj.org-article:87fea8adbb2d48459e1a3a7221f975472021-11-25T05:46:25ZThe MogR transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in Listeria monocytogenes.1553-73661553-737410.1371/journal.ppat.0020030https://doaj.org/article/87fea8adbb2d48459e1a3a7221f975472006-04-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.0020030https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Flagella are surface structures critical for motility and virulence of many bacterial species. In Listeria monocytogenes, MogR tightly represses expression of flagellin (FlaA) during extracellular growth at 37 degrees C and during intracellular infection. MogR is also required for full virulence in a murine model of infection. Using in vitro and in vivo infection models, we determined that the severe virulence defect of MogR-negative bacteria is due to overexpression of FlaA. Specifically, overproduction of FlaA in MogR-negative bacteria caused pleiotropic defects in bacterial division (chaining phenotype), intracellular spread, and virulence in mice. DNA binding and microarray analyses revealed that MogR represses transcription of all known flagellar motility genes by binding directly to a minimum of two TTTT-N5-AAAA recognition sites positioned within promoter regions such that RNA polymerase binding is occluded. Analysis of MogR protein levels demonstrated that modulation of MogR repression activity confers the temperature-specificity to flagellar motility gene expression. Epistasis analysis revealed that MogR repression of transcription is antagonized in a temperature-dependent manner by the DegU response regulator and that DegU further regulates FlaA levels through a posttranscriptional mechanism. These studies provide the first known example to our knowledge of a transcriptional repressor functioning as a master regulator controlling nonhierarchal expression of flagellar motility genes.Aimee ShenDarren E HigginsPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 2, Iss 4, p e30 (2006)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Aimee Shen Darren E Higgins The MogR transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in Listeria monocytogenes.
description	Flagella are surface structures critical for motility and virulence of many bacterial species. In Listeria monocytogenes, MogR tightly represses expression of flagellin (FlaA) during extracellular growth at 37 degrees C and during intracellular infection. MogR is also required for full virulence in a murine model of infection. Using in vitro and in vivo infection models, we determined that the severe virulence defect of MogR-negative bacteria is due to overexpression of FlaA. Specifically, overproduction of FlaA in MogR-negative bacteria caused pleiotropic defects in bacterial division (chaining phenotype), intracellular spread, and virulence in mice. DNA binding and microarray analyses revealed that MogR represses transcription of all known flagellar motility genes by binding directly to a minimum of two TTTT-N5-AAAA recognition sites positioned within promoter regions such that RNA polymerase binding is occluded. Analysis of MogR protein levels demonstrated that modulation of MogR repression activity confers the temperature-specificity to flagellar motility gene expression. Epistasis analysis revealed that MogR repression of transcription is antagonized in a temperature-dependent manner by the DegU response regulator and that DegU further regulates FlaA levels through a posttranscriptional mechanism. These studies provide the first known example to our knowledge of a transcriptional repressor functioning as a master regulator controlling nonhierarchal expression of flagellar motility genes.
format	article
author	Aimee Shen Darren E Higgins
author_facet	Aimee Shen Darren E Higgins
author_sort	Aimee Shen
title	The MogR transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in Listeria monocytogenes.
title_short	The MogR transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in Listeria monocytogenes.
title_full	The MogR transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in Listeria monocytogenes.
title_fullStr	The MogR transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in Listeria monocytogenes.
title_full_unstemmed	The MogR transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in Listeria monocytogenes.
title_sort	mogr transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in listeria monocytogenes.
publisher	Public Library of Science (PLoS)
publishDate	2006
url	https://doaj.org/article/87fea8adbb2d48459e1a3a7221f97547
work_keys_str_mv	AT aimeeshen themogrtranscriptionalrepressorregulatesnonhierarchalexpressionofflagellarmotilitygenesandvirulenceinlisteriamonocytogenes AT darrenehiggins themogrtranscriptionalrepressorregulatesnonhierarchalexpressionofflagellarmotilitygenesandvirulenceinlisteriamonocytogenes AT aimeeshen mogrtranscriptionalrepressorregulatesnonhierarchalexpressionofflagellarmotilitygenesandvirulenceinlisteriamonocytogenes AT darrenehiggins mogrtranscriptionalrepressorregulatesnonhierarchalexpressionofflagellarmotilitygenesandvirulenceinlisteriamonocytogenes
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The MogR transcriptional repressor regulates nonhierarchal expression of flagellar motility genes and virulence in Listeria monocytogenes.

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