Doxycycline Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy in Mice

After successful surgeries for patients with rhegmatogenous retinal detachment, the most common cause of retinal redetachment is proliferative vitreoretinopathy (PVR), which causes severe vision impairment and even blindness worldwide. Until now, the major treatment for PVR is surgical removal of th...

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Autores principales:	Shun-Hua Chen, Yu-Jheng Lin, Li-Chiu Wang, Hsien-Yang Tsai, Chang-Hao Yang, Yu-Ti Teng, Sheng-Min Hsu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	doxycycline proliferative vitreoretinopathy (PVR) rhegmatogenous retinal detachment (RRD) Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/8806277f6b544396bdb91f06fab6d627
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spelling	oai:doaj.org-article:8806277f6b544396bdb91f06fab6d6272021-11-11T17:08:07ZDoxycycline Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy in Mice10.3390/ijms2221116701422-00671661-6596https://doaj.org/article/8806277f6b544396bdb91f06fab6d6272021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11670https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067After successful surgeries for patients with rhegmatogenous retinal detachment, the most common cause of retinal redetachment is proliferative vitreoretinopathy (PVR), which causes severe vision impairment and even blindness worldwide. Until now, the major treatment for PVR is surgical removal of the epiretinal membrane, while effective treatment to prevent PVR is still unavailable. Therefore, we investigated the potential of doxycycline, an antibiotic in the tetracycline class, to treat PVR using a mouse model. We used the human retinal pigment epithelial cell line, ARPE-19, for in vitro and in vivo studies to test doxycycline for PVR treatment. We found that doxycycline suppressed the migration, proliferation, and contraction of ARPE-19 cells with reduced p38 MAPK activation and total MMP activity. Intravitreal doxycycline and topical tetracycline treatment significantly ameliorated the PVR severity induced by ARPE-19 cells in mice. PVR increased the expression of MMP-9 and IL-4 and p38 MAPK phosphorylation and modestly decreased IL-10. These effects were reversed by doxycycline and tetracycline treatment in the mouse retina. These results suggest that doxycycline will be a potential treatment for PVR in the future.Shun-Hua ChenYu-Jheng LinLi-Chiu WangHsien-Yang TsaiChang-Hao YangYu-Ti TengSheng-Min HsuMDPI AGarticledoxycyclineproliferative vitreoretinopathy (PVR)rhegmatogenous retinal detachment (RRD)Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11670, p 11670 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	doxycycline proliferative vitreoretinopathy (PVR) rhegmatogenous retinal detachment (RRD) Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	doxycycline proliferative vitreoretinopathy (PVR) rhegmatogenous retinal detachment (RRD) Biology (General) QH301-705.5 Chemistry QD1-999 Shun-Hua Chen Yu-Jheng Lin Li-Chiu Wang Hsien-Yang Tsai Chang-Hao Yang Yu-Ti Teng Sheng-Min Hsu Doxycycline Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy in Mice
description	After successful surgeries for patients with rhegmatogenous retinal detachment, the most common cause of retinal redetachment is proliferative vitreoretinopathy (PVR), which causes severe vision impairment and even blindness worldwide. Until now, the major treatment for PVR is surgical removal of the epiretinal membrane, while effective treatment to prevent PVR is still unavailable. Therefore, we investigated the potential of doxycycline, an antibiotic in the tetracycline class, to treat PVR using a mouse model. We used the human retinal pigment epithelial cell line, ARPE-19, for in vitro and in vivo studies to test doxycycline for PVR treatment. We found that doxycycline suppressed the migration, proliferation, and contraction of ARPE-19 cells with reduced p38 MAPK activation and total MMP activity. Intravitreal doxycycline and topical tetracycline treatment significantly ameliorated the PVR severity induced by ARPE-19 cells in mice. PVR increased the expression of MMP-9 and IL-4 and p38 MAPK phosphorylation and modestly decreased IL-10. These effects were reversed by doxycycline and tetracycline treatment in the mouse retina. These results suggest that doxycycline will be a potential treatment for PVR in the future.
format	article
author	Shun-Hua Chen Yu-Jheng Lin Li-Chiu Wang Hsien-Yang Tsai Chang-Hao Yang Yu-Ti Teng Sheng-Min Hsu
author_facet	Shun-Hua Chen Yu-Jheng Lin Li-Chiu Wang Hsien-Yang Tsai Chang-Hao Yang Yu-Ti Teng Sheng-Min Hsu
author_sort	Shun-Hua Chen
title	Doxycycline Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy in Mice
title_short	Doxycycline Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy in Mice
title_full	Doxycycline Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy in Mice
title_fullStr	Doxycycline Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy in Mice
title_full_unstemmed	Doxycycline Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy in Mice
title_sort	doxycycline ameliorates the severity of experimental proliferative vitreoretinopathy in mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8806277f6b544396bdb91f06fab6d627
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Doxycycline Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy in Mice

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