First identification of ITM2B interactome in the human retina

Abstract Integral Membrane Protein 2 B (ITM2B) is a type II ubiquitous transmembrane protein which role remains unclear. ITM2B mutations have been associated with different disorders: mutations leading to longer mutant proteins have been reported in two distinct Alzheimer-like autosomal dominant dis...

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Autores principales:	J. Wohlschlegel, M. Argentini, C. Michiels, C. Letellier, V. Forster, C. Condroyer, Z. He, G. Thuret, C. Zeitz, T. Léger, I. Audo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8808d894b8d94bb192c4750edd86a0ff
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spelling	oai:doaj.org-article:8808d894b8d94bb192c4750edd86a0ff2021-12-02T19:02:33ZFirst identification of ITM2B interactome in the human retina10.1038/s41598-021-96571-62045-2322https://doaj.org/article/8808d894b8d94bb192c4750edd86a0ff2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96571-6https://doaj.org/toc/2045-2322Abstract Integral Membrane Protein 2 B (ITM2B) is a type II ubiquitous transmembrane protein which role remains unclear. ITM2B mutations have been associated with different disorders: mutations leading to longer mutant proteins have been reported in two distinct Alzheimer-like autosomal dominant disorders with early-onset progressive dementia and cerebellar ataxia. Both disorders share neurological features including severe cerebral amyloid angiopathy, non-neuritic plaques, and fibrillary tangles as in Alzheimer disease. Our group reported a missense mutation in ITM2B, in an unusual retinal dystrophy with no dementia. This finding suggests a specific role of ITM2B in the retina. As the identification of retinal-specific ITM2B partners could bring new insights into the cellular functions of ITM2B, we performed quantitative proteomics of ITM2B interactome of the human retina. Overall, 457 ITM2B partners were identified with 8 of them involved in visual transduction. In addition, bulk Gene Ontology analyses showed that many ITM2B partners are involved in several other biological functions, such as microtubule organization, protein translation and interestingly, mitochondrial homeostasis. These data represent the first report of the ITM2B interactome in the human retina and may serve as a valuable inventory of new potential ITM2B partners for future investigations of ITM2B physiological functions and dysfunctions.J. WohlschlegelM. ArgentiniC. MichielsC. LetellierV. ForsterC. CondroyerZ. HeG. ThuretC. ZeitzT. LégerI. AudoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J. Wohlschlegel M. Argentini C. Michiels C. Letellier V. Forster C. Condroyer Z. He G. Thuret C. Zeitz T. Léger I. Audo First identification of ITM2B interactome in the human retina
description	Abstract Integral Membrane Protein 2 B (ITM2B) is a type II ubiquitous transmembrane protein which role remains unclear. ITM2B mutations have been associated with different disorders: mutations leading to longer mutant proteins have been reported in two distinct Alzheimer-like autosomal dominant disorders with early-onset progressive dementia and cerebellar ataxia. Both disorders share neurological features including severe cerebral amyloid angiopathy, non-neuritic plaques, and fibrillary tangles as in Alzheimer disease. Our group reported a missense mutation in ITM2B, in an unusual retinal dystrophy with no dementia. This finding suggests a specific role of ITM2B in the retina. As the identification of retinal-specific ITM2B partners could bring new insights into the cellular functions of ITM2B, we performed quantitative proteomics of ITM2B interactome of the human retina. Overall, 457 ITM2B partners were identified with 8 of them involved in visual transduction. In addition, bulk Gene Ontology analyses showed that many ITM2B partners are involved in several other biological functions, such as microtubule organization, protein translation and interestingly, mitochondrial homeostasis. These data represent the first report of the ITM2B interactome in the human retina and may serve as a valuable inventory of new potential ITM2B partners for future investigations of ITM2B physiological functions and dysfunctions.
format	article
author	J. Wohlschlegel M. Argentini C. Michiels C. Letellier V. Forster C. Condroyer Z. He G. Thuret C. Zeitz T. Léger I. Audo
author_facet	J. Wohlschlegel M. Argentini C. Michiels C. Letellier V. Forster C. Condroyer Z. He G. Thuret C. Zeitz T. Léger I. Audo
author_sort	J. Wohlschlegel
title	First identification of ITM2B interactome in the human retina
title_short	First identification of ITM2B interactome in the human retina
title_full	First identification of ITM2B interactome in the human retina
title_fullStr	First identification of ITM2B interactome in the human retina
title_full_unstemmed	First identification of ITM2B interactome in the human retina
title_sort	first identification of itm2b interactome in the human retina
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8808d894b8d94bb192c4750edd86a0ff
work_keys_str_mv	AT jwohlschlegel firstidentificationofitm2binteractomeinthehumanretina AT margentini firstidentificationofitm2binteractomeinthehumanretina AT cmichiels firstidentificationofitm2binteractomeinthehumanretina AT cletellier firstidentificationofitm2binteractomeinthehumanretina AT vforster firstidentificationofitm2binteractomeinthehumanretina AT ccondroyer firstidentificationofitm2binteractomeinthehumanretina AT zhe firstidentificationofitm2binteractomeinthehumanretina AT gthuret firstidentificationofitm2binteractomeinthehumanretina AT czeitz firstidentificationofitm2binteractomeinthehumanretina AT tleger firstidentificationofitm2binteractomeinthehumanretina AT iaudo firstidentificationofitm2binteractomeinthehumanretina
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First identification of ITM2B interactome in the human retina

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