THE STUDY OF REGULARITIES OF INDUCTION OF IL-8 SYNTHESIS BY NEUTROPHIL DEFENSINS IN VITRO

Abstract. We explored the effects of defensins on IL-8 synthesis in various human cells, including bronchoepithelial cell line A-549, monocytes, monocyte-derived macrophages differentiated in the presence of M-CSF, HU-VEC cells, HEK293 and THP-1 cell lines. HNP at 10-5-10-4М induced IL-8 production...

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Autores principales:	Y. V. Chaly, K. V. Kotlinsky, A. M. Sholukh, N. N. Voitenok
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	monocytes macrophages il–8 human alpha-defensins Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/880b8b2d61974710ad57749df0b2ca1e
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spelling	oai:doaj.org-article:880b8b2d61974710ad57749df0b2ca1e2021-11-18T08:03:43ZTHE STUDY OF REGULARITIES OF INDUCTION OF IL-8 SYNTHESIS BY NEUTROPHIL DEFENSINS IN VITRO1563-06252313-741X10.15789/1563-0625-2005-5-6-579-582https://doaj.org/article/880b8b2d61974710ad57749df0b2ca1e2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/630https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. We explored the effects of defensins on IL-8 synthesis in various human cells, including bronchoepithelial cell line A-549, monocytes, monocyte-derived macrophages differentiated in the presence of M-CSF, HU-VEC cells, HEK293 and THP-1 cell lines. HNP at 10-5-10-4М induced IL-8 production and cytotoxicity in serum-less A-549 culture. The addition of serum abrogated the cytotoxicity along with the induction of IL-8 synthesis. Similar effects were observed in HEK293 cell line and HUVEC.The induction of cytotoxicity along with IL-8 production by HNP at 10-5-10-4М was also observed in the serum-less cultures of human monocytes and macrophages. However, monocytes and macrophages retained HNP-induced production of IL-8 after the addition of serum, while cytotoxic effect of HNP was completely inhibited. The data imply a specific interaction of alpha-defensins with some membrane receptors of monocytes and macrophages that leads to the induction of IL-8 and might also occur in vivo.Y. V. ChalyK. V. KotlinskyA. M. SholukhN. N. VoitenokSPb RAACIarticlemonocytesmacrophagesil–8human alpha-defensinsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 7, Iss 5-6, Pp 579-582 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	monocytes macrophages il–8 human alpha-defensins Immunologic diseases. Allergy RC581-607
spellingShingle	monocytes macrophages il–8 human alpha-defensins Immunologic diseases. Allergy RC581-607 Y. V. Chaly K. V. Kotlinsky A. M. Sholukh N. N. Voitenok THE STUDY OF REGULARITIES OF INDUCTION OF IL-8 SYNTHESIS BY NEUTROPHIL DEFENSINS IN VITRO
description	Abstract. We explored the effects of defensins on IL-8 synthesis in various human cells, including bronchoepithelial cell line A-549, monocytes, monocyte-derived macrophages differentiated in the presence of M-CSF, HU-VEC cells, HEK293 and THP-1 cell lines. HNP at 10-5-10-4М induced IL-8 production and cytotoxicity in serum-less A-549 culture. The addition of serum abrogated the cytotoxicity along with the induction of IL-8 synthesis. Similar effects were observed in HEK293 cell line and HUVEC.The induction of cytotoxicity along with IL-8 production by HNP at 10-5-10-4М was also observed in the serum-less cultures of human monocytes and macrophages. However, monocytes and macrophages retained HNP-induced production of IL-8 after the addition of serum, while cytotoxic effect of HNP was completely inhibited. The data imply a specific interaction of alpha-defensins with some membrane receptors of monocytes and macrophages that leads to the induction of IL-8 and might also occur in vivo.
format	article
author	Y. V. Chaly K. V. Kotlinsky A. M. Sholukh N. N. Voitenok
author_facet	Y. V. Chaly K. V. Kotlinsky A. M. Sholukh N. N. Voitenok
author_sort	Y. V. Chaly
title	THE STUDY OF REGULARITIES OF INDUCTION OF IL-8 SYNTHESIS BY NEUTROPHIL DEFENSINS IN VITRO
title_short	THE STUDY OF REGULARITIES OF INDUCTION OF IL-8 SYNTHESIS BY NEUTROPHIL DEFENSINS IN VITRO
title_full	THE STUDY OF REGULARITIES OF INDUCTION OF IL-8 SYNTHESIS BY NEUTROPHIL DEFENSINS IN VITRO
title_fullStr	THE STUDY OF REGULARITIES OF INDUCTION OF IL-8 SYNTHESIS BY NEUTROPHIL DEFENSINS IN VITRO
title_full_unstemmed	THE STUDY OF REGULARITIES OF INDUCTION OF IL-8 SYNTHESIS BY NEUTROPHIL DEFENSINS IN VITRO
title_sort	study of regularities of induction of il-8 synthesis by neutrophil defensins in vitro
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/880b8b2d61974710ad57749df0b2ca1e
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THE STUDY OF REGULARITIES OF INDUCTION OF IL-8 SYNTHESIS BY NEUTROPHIL DEFENSINS IN VITRO

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