Allogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine

Kevin Yaneselli,1 Andrea Filomeno,1 Gabriel Semiglia,1 Carolina Arce,1 Analía Rial,2 Natalia Muñoz,2 María Moreno,2 Kent Erickson,3 Jacqueline Maisonnave11Universidad de la República, Facultad de Veterinaria, Montevideo, Uruguay; 2Laboratory for Vaccine Re...

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Autores principales:	Yaneselli K, Filomeno A, Semiglia G, Arce C, Rial A, Mu&ntilde;oz N, Moreno M, Erickson K, Maisonnave J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/880c31beea294a4fb058438f0bb4f4cd
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spelling	oai:doaj.org-article:880c31beea294a4fb058438f0bb4f4cd2021-12-02T03:42:15ZAllogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine2230-2034https://doaj.org/article/880c31beea294a4fb058438f0bb4f4cd2013-10-01T00:00:00Zhttp://www.dovepress.com/allogeneic-stem-cell-transplantation-for-bone-regeneration-of-a-nonuni-a14626https://doaj.org/toc/2230-2034Kevin Yaneselli,1 Andrea Filomeno,1 Gabriel Semiglia,1 Carolina Arce,1 Analía Rial,2 Natalia Muñoz,2 María Moreno,2 Kent Erickson,3 Jacqueline Maisonnave11Universidad de la República, Facultad de Veterinaria, Montevideo, Uruguay; 2Laboratory for Vaccine Research, Department of Biotechnology, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay; 3University of California, Davis, CA, USAAbstract: Nonunion bone defects occur frequently with local pain, functional limitations, muscular atrophy, and fistulas due to osteomyelitis. The application of mesenchymal stem cells (MSCs) could improve regeneration of bone following bone defects. The objective of the present study was to evaluate the treatment of a nonunion defect due to chronic osteomyelitis in a greyhound female dog with allogeneic adipose tissue-derived mesenchymal stem cells (AT-MSCs). The implanted cells were adherent to plastic, were of fibroblast type, and expressed the canine stem cell markers CD90low, CD44high, and CD45-. Cell therapy consisted of five percutaneous weekly injections of 2 × 106 allogeneic AT-MSCs into the bone defect (total of 10 × 106 AT-MSCs). The patient was evaluated clinically and radiologically for up to 1 year. The results were clinical improvement, a light lameness score of 1 at week 16, return to use of its forearm, no pain, and increased muscular mass. No signs of osteomyelitis were observed radiologically and clinically there were no fistulas. There was no evidence of local or systemic adverse reactions caused by the aloimplants. The clinical relevance of the cell therapy contributing to repair of bone defects in small animals is a very promising future alternative. These results may have an important impact in new regenerative treatments for animal and human orthopedics.Keywords: allogeneic, AT-MSCs, treatment, nonunion, canineYaneselli KFilomeno ASemiglia GArce CRial AMu&ntilde;oz NMoreno MErickson KMaisonnave JDove Medical PressarticleVeterinary medicineSF600-1100ENVeterinary Medicine: Research and Reports, Vol 2013, Iss default, Pp 39-44 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Veterinary medicine SF600-1100
spellingShingle	Veterinary medicine SF600-1100 Yaneselli K Filomeno A Semiglia G Arce C Rial A Mu&ntilde;oz N Moreno M Erickson K Maisonnave J Allogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine
description	Kevin Yaneselli,1 Andrea Filomeno,1 Gabriel Semiglia,1 Carolina Arce,1 Analía Rial,2 Natalia Muñoz,2 María Moreno,2 Kent Erickson,3 Jacqueline Maisonnave11Universidad de la República, Facultad de Veterinaria, Montevideo, Uruguay; 2Laboratory for Vaccine Research, Department of Biotechnology, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay; 3University of California, Davis, CA, USAAbstract: Nonunion bone defects occur frequently with local pain, functional limitations, muscular atrophy, and fistulas due to osteomyelitis. The application of mesenchymal stem cells (MSCs) could improve regeneration of bone following bone defects. The objective of the present study was to evaluate the treatment of a nonunion defect due to chronic osteomyelitis in a greyhound female dog with allogeneic adipose tissue-derived mesenchymal stem cells (AT-MSCs). The implanted cells were adherent to plastic, were of fibroblast type, and expressed the canine stem cell markers CD90low, CD44high, and CD45-. Cell therapy consisted of five percutaneous weekly injections of 2 × 106 allogeneic AT-MSCs into the bone defect (total of 10 × 106 AT-MSCs). The patient was evaluated clinically and radiologically for up to 1 year. The results were clinical improvement, a light lameness score of 1 at week 16, return to use of its forearm, no pain, and increased muscular mass. No signs of osteomyelitis were observed radiologically and clinically there were no fistulas. There was no evidence of local or systemic adverse reactions caused by the aloimplants. The clinical relevance of the cell therapy contributing to repair of bone defects in small animals is a very promising future alternative. These results may have an important impact in new regenerative treatments for animal and human orthopedics.Keywords: allogeneic, AT-MSCs, treatment, nonunion, canine
format	article
author	Yaneselli K Filomeno A Semiglia G Arce C Rial A Mu&ntilde;oz N Moreno M Erickson K Maisonnave J
author_facet	Yaneselli K Filomeno A Semiglia G Arce C Rial A Mu&ntilde;oz N Moreno M Erickson K Maisonnave J
author_sort	Yaneselli K
title	Allogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine
title_short	Allogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine
title_full	Allogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine
title_fullStr	Allogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine
title_full_unstemmed	Allogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine
title_sort	allogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/880c31beea294a4fb058438f0bb4f4cd
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Allogeneic stem cell transplantation for bone regeneration of a nonunion defect in a canine

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