Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells

Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells

Jong Kyu Hong,1,2 Ju Yup Bang,3 Guan Xu,4 Jun-Hee Lee,1 Yeon-Ju Kim,1 Ho-Jun Lee,5 Han Seong Kim,3 Sang-Mo Kwon1,2,6 1Laboratory for Vascular Medicine and Stem Cell Biology, Medical Research Institute, Department of Physiology, School of Medicine, Pusan National University, Yangsan, South...

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Autores principales: Hong JK, Bang JY, Xu G, Lee JH, Kim YJ, Lee HJ, Kim HS, Kwon SM
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/880c85a7feab4631903c402162e34d4a
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spelling oai:doaj.org-article:880c85a7feab4631903c402162e34d4a2021-12-02T00:20:48ZThickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells1178-2013https://doaj.org/article/880c85a7feab4631903c402162e34d4a2015-02-01T00:00:00Zhttp://www.dovepress.com/thickness-controllable-electrospun-fibers-promote-tubular-structure-fo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Jong Kyu Hong,1,2 Ju Yup Bang,3 Guan Xu,4 Jun-Hee Lee,1 Yeon-Ju Kim,1 Ho-Jun Lee,5 Han Seong Kim,3 Sang-Mo Kwon1,2,6 1Laboratory for Vascular Medicine and Stem Cell Biology, Medical Research Institute, Department of Physiology, School of Medicine, Pusan National University, Yangsan, South Korea; 2Conversence Stem Cell Research Center, Medical Research Institute, School of Medicine, Pusan National University, Yangsan, South Korea; 3Department of Organic Material Science, Pusan National University, Geumjeong-gu, Busan, South Korea; 4Department of Radiology, School of Medicine, University of Michigan, Ann Arbor, MI, USA; 5Department of Electrical Engineering, Pusan National University, Geumjeong-gu, Busan, South Korea; 6Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Department of Physiology, Pusan National University School of Medicine, Yangsan, South Korea Abstract: Controlling the thickness of an electrospun nanofibrous scaffold by altering its pore size has been shown to regulate cell behaviors such as cell infiltration into a three-dimensional (3D) scaffold. This is of great importance when manufacturing tissue-engineering scaffolds using an electrospinning process. In this study, we report the development of a novel process whereby additional aluminum foil layers were applied to the accumulated electrospun fibers of an existing aluminum foil collector, effectively reducing the incidence of charge buildup. Using this process, we fabricated an electrospun scaffold with a large pore (pore size >40 µm) while simultaneously controlling the thickness. We demonstrate that the large pore size triggered rapid infiltration (160 µm in 4 hours of cell culture) of individual endothelial progenitor cells (EPCs) and rapid cell colonization after seeding EPC spheroids. We confirmed that the 3D, but not two-dimensional, scaffold structures regulated tubular structure formation by the EPCs. Thus, incorporation of stem cells into a highly porous 3D scaffold with tunable thickness has implications for the regeneration of vascularized thick tissues and cardiac patch development. Keywords: electrospinning, nanofibrous scaffold, tunable thickness, vascularization, stem cellHong JKBang JYXu GLee JHKim YJLee HJKim HSKwon SMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1189-1200 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Hong JK
Bang JY
Xu G
Lee JH
Kim YJ
Lee HJ
Kim HS
Kwon SM
Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells
description Jong Kyu Hong,1,2 Ju Yup Bang,3 Guan Xu,4 Jun-Hee Lee,1 Yeon-Ju Kim,1 Ho-Jun Lee,5 Han Seong Kim,3 Sang-Mo Kwon1,2,6 1Laboratory for Vascular Medicine and Stem Cell Biology, Medical Research Institute, Department of Physiology, School of Medicine, Pusan National University, Yangsan, South Korea; 2Conversence Stem Cell Research Center, Medical Research Institute, School of Medicine, Pusan National University, Yangsan, South Korea; 3Department of Organic Material Science, Pusan National University, Geumjeong-gu, Busan, South Korea; 4Department of Radiology, School of Medicine, University of Michigan, Ann Arbor, MI, USA; 5Department of Electrical Engineering, Pusan National University, Geumjeong-gu, Busan, South Korea; 6Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Department of Physiology, Pusan National University School of Medicine, Yangsan, South Korea Abstract: Controlling the thickness of an electrospun nanofibrous scaffold by altering its pore size has been shown to regulate cell behaviors such as cell infiltration into a three-dimensional (3D) scaffold. This is of great importance when manufacturing tissue-engineering scaffolds using an electrospinning process. In this study, we report the development of a novel process whereby additional aluminum foil layers were applied to the accumulated electrospun fibers of an existing aluminum foil collector, effectively reducing the incidence of charge buildup. Using this process, we fabricated an electrospun scaffold with a large pore (pore size >40 µm) while simultaneously controlling the thickness. We demonstrate that the large pore size triggered rapid infiltration (160 µm in 4 hours of cell culture) of individual endothelial progenitor cells (EPCs) and rapid cell colonization after seeding EPC spheroids. We confirmed that the 3D, but not two-dimensional, scaffold structures regulated tubular structure formation by the EPCs. Thus, incorporation of stem cells into a highly porous 3D scaffold with tunable thickness has implications for the regeneration of vascularized thick tissues and cardiac patch development. Keywords: electrospinning, nanofibrous scaffold, tunable thickness, vascularization, stem cell
format article
author Hong JK
Bang JY
Xu G
Lee JH
Kim YJ
Lee HJ
Kim HS
Kwon SM
author_facet Hong JK
Bang JY
Xu G
Lee JH
Kim YJ
Lee HJ
Kim HS
Kwon SM
author_sort Hong JK
title Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells
title_short Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells
title_full Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells
title_fullStr Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells
title_full_unstemmed Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells
title_sort thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/880c85a7feab4631903c402162e34d4a
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AT bangjy thicknesscontrollableelectrospunfiberspromotetubularstructureformationbynbspendothelialprogenitorcells
AT xug thicknesscontrollableelectrospunfiberspromotetubularstructureformationbynbspendothelialprogenitorcells
AT leejh thicknesscontrollableelectrospunfiberspromotetubularstructureformationbynbspendothelialprogenitorcells
AT kimyj thicknesscontrollableelectrospunfiberspromotetubularstructureformationbynbspendothelialprogenitorcells
AT leehj thicknesscontrollableelectrospunfiberspromotetubularstructureformationbynbspendothelialprogenitorcells
AT kimhs thicknesscontrollableelectrospunfiberspromotetubularstructureformationbynbspendothelialprogenitorcells
AT kwonsm thicknesscontrollableelectrospunfiberspromotetubularstructureformationbynbspendothelialprogenitorcells
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