Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds

Abstract The appreciation that cell interactions in tissues is dependent on their three dimensional (3D) distribution has stimulated the development of 3D cell culture models. We constructed an artificial 3D tumour by culturing human breast cancer JIMT-1 cells and human dermal fibroblasts (HDFs) in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Atena Malakpour-Permlid, Irina Buzzi, Cecilia Hegardt, Fredrik Johansson, Stina Oredsson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88151e5832c749809f828ff0ec29c938
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:88151e5832c749809f828ff0ec29c938
record_format	dspace
spelling	oai:doaj.org-article:88151e5832c749809f828ff0ec29c9382021-12-02T11:45:03ZIdentification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds10.1038/s41598-021-85742-02045-2322https://doaj.org/article/88151e5832c749809f828ff0ec29c9382021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85742-0https://doaj.org/toc/2045-2322Abstract The appreciation that cell interactions in tissues is dependent on their three dimensional (3D) distribution has stimulated the development of 3D cell culture models. We constructed an artificial 3D tumour by culturing human breast cancer JIMT-1 cells and human dermal fibroblasts (HDFs) in a 3D network of electrospun polycaprolactone fibres. Here, we investigate ECM components produced by the cells in the artificial 3D tumour, which is an important step in validating the model. Immunostaining and confocal fluorescence microscopy show that the ECM proteins fibronectin, collagen I, and laminin are deposited throughout the entire 3D structure. Secreted soluble factors including matrix metalloproteinases (MMPs) and interleukine-6 (IL-6) were analysed in collected medium and were found to be mainly derived from the HDFs. Treatment with transforming growth factor-β1 (TGF-β1), a major cytokine found in a tumour, significantly alters the MMP activity and IL-6 concentration. In addition, TGF-β1 treatment, changes the morphology of the HDFs to become more elongated and with increased linearized actin filaments compared to non-treated HDFs. Collectively, these novel findings suggest that the artificial 3D tumour displays a clear cell distribution and ECM deposition that resembles a tumour environment in vivo, suggesting an innovative biological model to study a human tumour.Atena Malakpour-PermlidIrina BuzziCecilia HegardtFredrik JohanssonStina OredssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Atena Malakpour-Permlid Irina Buzzi Cecilia Hegardt Fredrik Johansson Stina Oredsson Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds
description	Abstract The appreciation that cell interactions in tissues is dependent on their three dimensional (3D) distribution has stimulated the development of 3D cell culture models. We constructed an artificial 3D tumour by culturing human breast cancer JIMT-1 cells and human dermal fibroblasts (HDFs) in a 3D network of electrospun polycaprolactone fibres. Here, we investigate ECM components produced by the cells in the artificial 3D tumour, which is an important step in validating the model. Immunostaining and confocal fluorescence microscopy show that the ECM proteins fibronectin, collagen I, and laminin are deposited throughout the entire 3D structure. Secreted soluble factors including matrix metalloproteinases (MMPs) and interleukine-6 (IL-6) were analysed in collected medium and were found to be mainly derived from the HDFs. Treatment with transforming growth factor-β1 (TGF-β1), a major cytokine found in a tumour, significantly alters the MMP activity and IL-6 concentration. In addition, TGF-β1 treatment, changes the morphology of the HDFs to become more elongated and with increased linearized actin filaments compared to non-treated HDFs. Collectively, these novel findings suggest that the artificial 3D tumour displays a clear cell distribution and ECM deposition that resembles a tumour environment in vivo, suggesting an innovative biological model to study a human tumour.
format	article
author	Atena Malakpour-Permlid Irina Buzzi Cecilia Hegardt Fredrik Johansson Stina Oredsson
author_facet	Atena Malakpour-Permlid Irina Buzzi Cecilia Hegardt Fredrik Johansson Stina Oredsson
author_sort	Atena Malakpour-Permlid
title	Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds
title_short	Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds
title_full	Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds
title_fullStr	Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds
title_full_unstemmed	Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds
title_sort	identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3d electrospun scaffolds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/88151e5832c749809f828ff0ec29c938
work_keys_str_mv	AT atenamalakpourpermlid identificationofextracellularmatrixproteinssecretedbyhumandermalfibroblastsculturedin3delectrospunscaffolds AT irinabuzzi identificationofextracellularmatrixproteinssecretedbyhumandermalfibroblastsculturedin3delectrospunscaffolds AT ceciliahegardt identificationofextracellularmatrixproteinssecretedbyhumandermalfibroblastsculturedin3delectrospunscaffolds AT fredrikjohansson identificationofextracellularmatrixproteinssecretedbyhumandermalfibroblastsculturedin3delectrospunscaffolds AT stinaoredsson identificationofextracellularmatrixproteinssecretedbyhumandermalfibroblastsculturedin3delectrospunscaffolds
_version_	1718395292106096640

Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds

Ejemplares similares