CRISPR Element Patterns vs. Pathoadaptability of Clinical <i>Pseudomonas aeruginosa</i> Isolates from a Medical Center in Moscow, Russia

<i>Pseudomonas aeruginosa</i> is a member of the ESKAPE opportunistic pathogen group, which includes six species of the most dangerous microbes. This pathogen is characterized by the rapid acquisition of antimicrobial resistance, thus causing major healthcare concerns. This study present...

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Autores principales:	Marina Tyumentseva, Yulia Mikhaylova, Anna Prelovskaya, Konstantin Karbyshev, Aleksandr Tyumentsev, Lyudmila Petrova, Anna Mironova, Mikhail Zamyatin, Andrey Shelenkov, Vasiliy Akimkin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pseudomonas aeruginosa</i> WGS antibiotic resistance virulence factors multiple CRISPR/Cas systems pathoadaptability Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/881d0d67073849baac51f0b52f26051a
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spelling	oai:doaj.org-article:881d0d67073849baac51f0b52f26051a2021-11-25T16:22:21ZCRISPR Element Patterns vs. Pathoadaptability of Clinical <i>Pseudomonas aeruginosa</i> Isolates from a Medical Center in Moscow, Russia10.3390/antibiotics101113012079-6382https://doaj.org/article/881d0d67073849baac51f0b52f26051a2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1301https://doaj.org/toc/2079-6382<i>Pseudomonas aeruginosa</i> is a member of the ESKAPE opportunistic pathogen group, which includes six species of the most dangerous microbes. This pathogen is characterized by the rapid acquisition of antimicrobial resistance, thus causing major healthcare concerns. This study presents a comprehensive analysis of clinical <i>P. aeruginosa</i> isolates based on whole-genome sequencing data. The isolate collection studied was characterized by a variety of clonal lineages with a domination of high-risk epidemic clones and different CRISPR/Cas element patterns. This is the first report on the coexistence of two and even three different types of CRISPR/Cas systems simultaneously in Russian clinical strains of <i>P. aeruginosa</i>. The data include molecular typing and genotypic antibiotic resistance determination, as well as the phylogenetic analysis of the full-length <i>cas</i> gene and anti-CRISPR genes sequences, predicted prophage sequences, and conducted a detailed CRISPR array analysis. The differences between the isolates carrying different types and quantities of CRISPR/Cas systems were investigated. The pattern of virulence factors in <i>P. aeruginosa</i> isolates lacking putative CRISPR/Cas systems significantly differed from that of samples with single or multiple putative CRISPR/Cas systems. We found significant correlations between the numbers of prophage sequences, antibiotic resistance genes, and virulence genes in <i>P. aeruginosa</i> isolates with different patterns of CRISPR/Cas-elements. We believe that the data presented will contribute to further investigations in the field of bacterial pathoadaptability, including antimicrobial resistance and the role of CRISPR/Cas systems in the plasticity of the <i>P. aeruginosa</i> genome.Marina TyumentsevaYulia MikhaylovaAnna PrelovskayaKonstantin KarbyshevAleksandr TyumentsevLyudmila PetrovaAnna MironovaMikhail ZamyatinAndrey ShelenkovVasiliy AkimkinMDPI AGarticle<i>Pseudomonas aeruginosa</i>WGSantibiotic resistancevirulence factorsmultiple CRISPR/Cas systemspathoadaptabilityTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1301, p 1301 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pseudomonas aeruginosa</i> WGS antibiotic resistance virulence factors multiple CRISPR/Cas systems pathoadaptability Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Pseudomonas aeruginosa</i> WGS antibiotic resistance virulence factors multiple CRISPR/Cas systems pathoadaptability Therapeutics. Pharmacology RM1-950 Marina Tyumentseva Yulia Mikhaylova Anna Prelovskaya Konstantin Karbyshev Aleksandr Tyumentsev Lyudmila Petrova Anna Mironova Mikhail Zamyatin Andrey Shelenkov Vasiliy Akimkin CRISPR Element Patterns vs. Pathoadaptability of Clinical <i>Pseudomonas aeruginosa</i> Isolates from a Medical Center in Moscow, Russia
description	<i>Pseudomonas aeruginosa</i> is a member of the ESKAPE opportunistic pathogen group, which includes six species of the most dangerous microbes. This pathogen is characterized by the rapid acquisition of antimicrobial resistance, thus causing major healthcare concerns. This study presents a comprehensive analysis of clinical <i>P. aeruginosa</i> isolates based on whole-genome sequencing data. The isolate collection studied was characterized by a variety of clonal lineages with a domination of high-risk epidemic clones and different CRISPR/Cas element patterns. This is the first report on the coexistence of two and even three different types of CRISPR/Cas systems simultaneously in Russian clinical strains of <i>P. aeruginosa</i>. The data include molecular typing and genotypic antibiotic resistance determination, as well as the phylogenetic analysis of the full-length <i>cas</i> gene and anti-CRISPR genes sequences, predicted prophage sequences, and conducted a detailed CRISPR array analysis. The differences between the isolates carrying different types and quantities of CRISPR/Cas systems were investigated. The pattern of virulence factors in <i>P. aeruginosa</i> isolates lacking putative CRISPR/Cas systems significantly differed from that of samples with single or multiple putative CRISPR/Cas systems. We found significant correlations between the numbers of prophage sequences, antibiotic resistance genes, and virulence genes in <i>P. aeruginosa</i> isolates with different patterns of CRISPR/Cas-elements. We believe that the data presented will contribute to further investigations in the field of bacterial pathoadaptability, including antimicrobial resistance and the role of CRISPR/Cas systems in the plasticity of the <i>P. aeruginosa</i> genome.
format	article
author	Marina Tyumentseva Yulia Mikhaylova Anna Prelovskaya Konstantin Karbyshev Aleksandr Tyumentsev Lyudmila Petrova Anna Mironova Mikhail Zamyatin Andrey Shelenkov Vasiliy Akimkin
author_facet	Marina Tyumentseva Yulia Mikhaylova Anna Prelovskaya Konstantin Karbyshev Aleksandr Tyumentsev Lyudmila Petrova Anna Mironova Mikhail Zamyatin Andrey Shelenkov Vasiliy Akimkin
author_sort	Marina Tyumentseva
title	CRISPR Element Patterns vs. Pathoadaptability of Clinical <i>Pseudomonas aeruginosa</i> Isolates from a Medical Center in Moscow, Russia
title_short	CRISPR Element Patterns vs. Pathoadaptability of Clinical <i>Pseudomonas aeruginosa</i> Isolates from a Medical Center in Moscow, Russia
title_full	CRISPR Element Patterns vs. Pathoadaptability of Clinical <i>Pseudomonas aeruginosa</i> Isolates from a Medical Center in Moscow, Russia
title_fullStr	CRISPR Element Patterns vs. Pathoadaptability of Clinical <i>Pseudomonas aeruginosa</i> Isolates from a Medical Center in Moscow, Russia
title_full_unstemmed	CRISPR Element Patterns vs. Pathoadaptability of Clinical <i>Pseudomonas aeruginosa</i> Isolates from a Medical Center in Moscow, Russia
title_sort	crispr element patterns vs. pathoadaptability of clinical <i>pseudomonas aeruginosa</i> isolates from a medical center in moscow, russia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/881d0d67073849baac51f0b52f26051a
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CRISPR Element Patterns vs. Pathoadaptability of Clinical <i>Pseudomonas aeruginosa</i> Isolates from a Medical Center in Moscow, Russia

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