Comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study.

We analyzed 4,352 participant- and staff-collected respiratory specimens from 2,796 subjects in the Oregon Child Absenteeism due to Respiratory Disease Study. Trained staff collected oropharyngeal specimens from school-aged children with acute respiratory illness while household participants of all...

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Autores principales:	Mitchell T Arnold, Jonathan L Temte, Shari K Barlow, Cristalyne J Bell, Maureen D Goss, Emily G Temte, Mary M Checovich, Erik Reisdorf, Samantha Scott, Kyley Guenther, Mary Wedig, Peter Shult, Amra Uzicanin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/881e1a1f41194e7fb842199c6620e1fc
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spelling	oai:doaj.org-article:881e1a1f41194e7fb842199c6620e1fc2021-12-02T20:18:57ZComparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study.1932-620310.1371/journal.pone.0239000https://doaj.org/article/881e1a1f41194e7fb842199c6620e1fc2020-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0239000https://doaj.org/toc/1932-6203We analyzed 4,352 participant- and staff-collected respiratory specimens from 2,796 subjects in the Oregon Child Absenteeism due to Respiratory Disease Study. Trained staff collected oropharyngeal specimens from school-aged children with acute respiratory illness while household participants of all ages collected their own midturbinate nasal specimens in year one and anterior nasal specimens in year two. Human ribonuclease P levels were measured using RT-PCR for all staff- and participant-collected specimens to determine adequacy, defined as Cycle threshold less than 38. Overall, staff- and participant-collected specimens were 99.9% and 96.4% adequate, respectively. Participant-collected midturbinate specimens were 95.2% adequate in year one, increasing to 97.2% in year two with anterior nasal collection. The mean human ribonuclease P Cycle threshold for participant-collected specimens was 31.18 in year one and 28.48 in year two. The results from this study suggest that community-based participant collection of respiratory specimens is comparable to staff-collected oropharyngeal specimens, is feasible, and may be optimal with anterior nasal collection.Mitchell T ArnoldJonathan L TemteShari K BarlowCristalyne J BellMaureen D GossEmily G TemteMary M ChecovichErik ReisdorfSamantha ScottKyley GuentherMary WedigPeter ShultAmra UzicaninPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 15, Iss 10, p e0239000 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mitchell T Arnold Jonathan L Temte Shari K Barlow Cristalyne J Bell Maureen D Goss Emily G Temte Mary M Checovich Erik Reisdorf Samantha Scott Kyley Guenther Mary Wedig Peter Shult Amra Uzicanin Comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study.
description	We analyzed 4,352 participant- and staff-collected respiratory specimens from 2,796 subjects in the Oregon Child Absenteeism due to Respiratory Disease Study. Trained staff collected oropharyngeal specimens from school-aged children with acute respiratory illness while household participants of all ages collected their own midturbinate nasal specimens in year one and anterior nasal specimens in year two. Human ribonuclease P levels were measured using RT-PCR for all staff- and participant-collected specimens to determine adequacy, defined as Cycle threshold less than 38. Overall, staff- and participant-collected specimens were 99.9% and 96.4% adequate, respectively. Participant-collected midturbinate specimens were 95.2% adequate in year one, increasing to 97.2% in year two with anterior nasal collection. The mean human ribonuclease P Cycle threshold for participant-collected specimens was 31.18 in year one and 28.48 in year two. The results from this study suggest that community-based participant collection of respiratory specimens is comparable to staff-collected oropharyngeal specimens, is feasible, and may be optimal with anterior nasal collection.
format	article
author	Mitchell T Arnold Jonathan L Temte Shari K Barlow Cristalyne J Bell Maureen D Goss Emily G Temte Mary M Checovich Erik Reisdorf Samantha Scott Kyley Guenther Mary Wedig Peter Shult Amra Uzicanin
author_facet	Mitchell T Arnold Jonathan L Temte Shari K Barlow Cristalyne J Bell Maureen D Goss Emily G Temte Mary M Checovich Erik Reisdorf Samantha Scott Kyley Guenther Mary Wedig Peter Shult Amra Uzicanin
author_sort	Mitchell T Arnold
title	Comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study.
title_short	Comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study.
title_full	Comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study.
title_fullStr	Comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study.
title_full_unstemmed	Comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study.
title_sort	comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease p detection with rt-pcr during a community-based study.
publisher	Public Library of Science (PLoS)
publishDate	2020
url	https://doaj.org/article/881e1a1f41194e7fb842199c6620e1fc
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Comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study.

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