Quantification of cell-bubble interactions in a 3D engineered tissue phantom

Abstract Understanding cell-bubble interactions is crucial for preventing bubble related pathologies and harnessing their potential therapeutic benefits. Bubbles can occur in the body as a result of therapeutic intravenous administration, surgery, infections or decompression. Subsequent interactions...

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Autores principales:	C. Walsh, N. Ovenden, E. Stride, U. Cheema
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88243497ecc642c0806f31ae79da40cc
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spelling	oai:doaj.org-article:88243497ecc642c0806f31ae79da40cc2021-12-02T16:06:07ZQuantification of cell-bubble interactions in a 3D engineered tissue phantom10.1038/s41598-017-06678-y2045-2322https://doaj.org/article/88243497ecc642c0806f31ae79da40cc2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06678-yhttps://doaj.org/toc/2045-2322Abstract Understanding cell-bubble interactions is crucial for preventing bubble related pathologies and harnessing their potential therapeutic benefits. Bubbles can occur in the body as a result of therapeutic intravenous administration, surgery, infections or decompression. Subsequent interactions with living cells, may result in pathological responses such as decompression sickness (DCS). This work investigates the interactions that occur between bubbles formed during decompression and cells in a 3D engineered tissue phantom. Increasing the tissue phantoms’ cellular density resulted in decreased dissolved O2 (DO) concentrations (p = 0.0003) measured using real-time O2 monitoring. Direct microscopic observation of these phantoms, revealed a significant (p = 0.0024) corresponding reduction in bubble nucleation. No significant difference in growth rate or maximum size of the bubbles was measured (p = 0.99 and 0.23). These results show that bubble nucleation is dominated by DO concentration (affected by cellular metabolism), rather than potential nucleation sites provided by cell-surfaces. Consequent bubble growth depends not only on DO concentration but also on competition for dissolved gas. Cell death was found to significantly increase (p = 0.0116) following a bubble-forming decompression. By comparison to 2D experiments; the more biomimetic 3D geometry and extracellular matrix in this work, provide data more applicable for understanding and developing models of in vivo bubble dynamics.C. WalshN. OvendenE. StrideU. CheemaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q C. Walsh N. Ovenden E. Stride U. Cheema Quantification of cell-bubble interactions in a 3D engineered tissue phantom
description	Abstract Understanding cell-bubble interactions is crucial for preventing bubble related pathologies and harnessing their potential therapeutic benefits. Bubbles can occur in the body as a result of therapeutic intravenous administration, surgery, infections or decompression. Subsequent interactions with living cells, may result in pathological responses such as decompression sickness (DCS). This work investigates the interactions that occur between bubbles formed during decompression and cells in a 3D engineered tissue phantom. Increasing the tissue phantoms’ cellular density resulted in decreased dissolved O2 (DO) concentrations (p = 0.0003) measured using real-time O2 monitoring. Direct microscopic observation of these phantoms, revealed a significant (p = 0.0024) corresponding reduction in bubble nucleation. No significant difference in growth rate or maximum size of the bubbles was measured (p = 0.99 and 0.23). These results show that bubble nucleation is dominated by DO concentration (affected by cellular metabolism), rather than potential nucleation sites provided by cell-surfaces. Consequent bubble growth depends not only on DO concentration but also on competition for dissolved gas. Cell death was found to significantly increase (p = 0.0116) following a bubble-forming decompression. By comparison to 2D experiments; the more biomimetic 3D geometry and extracellular matrix in this work, provide data more applicable for understanding and developing models of in vivo bubble dynamics.
format	article
author	C. Walsh N. Ovenden E. Stride U. Cheema
author_facet	C. Walsh N. Ovenden E. Stride U. Cheema
author_sort	C. Walsh
title	Quantification of cell-bubble interactions in a 3D engineered tissue phantom
title_short	Quantification of cell-bubble interactions in a 3D engineered tissue phantom
title_full	Quantification of cell-bubble interactions in a 3D engineered tissue phantom
title_fullStr	Quantification of cell-bubble interactions in a 3D engineered tissue phantom
title_full_unstemmed	Quantification of cell-bubble interactions in a 3D engineered tissue phantom
title_sort	quantification of cell-bubble interactions in a 3d engineered tissue phantom
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/88243497ecc642c0806f31ae79da40cc
work_keys_str_mv	AT cwalsh quantificationofcellbubbleinteractionsina3dengineeredtissuephantom AT novenden quantificationofcellbubbleinteractionsina3dengineeredtissuephantom AT estride quantificationofcellbubbleinteractionsina3dengineeredtissuephantom AT ucheema quantificationofcellbubbleinteractionsina3dengineeredtissuephantom
_version_	1718385098996318208

Quantification of cell-bubble interactions in a 3D engineered tissue phantom

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