Development of an immunochromatographic test strip for the detection of procaine in milk

Procaine (PRC) is a local anaesthetic used in the treatment of several disease related to ageing, asthma, and arthritis. In this study, we describe a rapid, sensitive, simple, and accurate method for the determination of PRC in milk, using an immunochromatographic test strip. For the immunochromatog...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kai Hao, Steven Suryoprabowo, Shanshan Song, Liqiang Liu, Qiankun Zheng, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	immunochromatographic assay local anaesthetic monoclonal antibody procaine strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/8831916419284c59ae4b6b197db87121
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8831916419284c59ae4b6b197db87121
record_format	dspace
spelling	oai:doaj.org-article:8831916419284c59ae4b6b197db871212021-11-26T11:19:47ZDevelopment of an immunochromatographic test strip for the detection of procaine in milk0954-01051465-344310.1080/09540105.2018.1523371https://doaj.org/article/8831916419284c59ae4b6b197db871212018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1523371https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Procaine (PRC) is a local anaesthetic used in the treatment of several disease related to ageing, asthma, and arthritis. In this study, we describe a rapid, sensitive, simple, and accurate method for the determination of PRC in milk, using an immunochromatographic test strip. For the immunochromatographic test, antigen (PRC-bovine serum albumin) and goat anti-mouse IgG were drawn onto a nitrocellulose membrane representing the control line and test line. The test was developed with a sensitive monoclonal antibody specific for PRC, generated by immunizing BALB/c mice with a well-characterized PRC- Keyhole limpet hemocyanin conjugate, produced in our laboratory. Under optimized conditions, the semi-quantitative test strip was able to detect PRC at concentrations down to 1 ng/mL in both 0.01 M phosphate buffer saline (pH 7.4) and milk samples. All results were obtained within 5 min. Our data indicate that the immunochromatographic assay is capable of sensitive, rapid, and specific on-site screening of PRC.Kai HaoSteven SuryoprabowoShanshan SongLiqiang LiuQiankun ZhengHua KuangTaylor & Francis Grouparticleimmunochromatographic assaylocal anaestheticmonoclonal antibodyprocainestrip testAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1150-1161 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunochromatographic assay local anaesthetic monoclonal antibody procaine strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	immunochromatographic assay local anaesthetic monoclonal antibody procaine strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Kai Hao Steven Suryoprabowo Shanshan Song Liqiang Liu Qiankun Zheng Hua Kuang Development of an immunochromatographic test strip for the detection of procaine in milk
description	Procaine (PRC) is a local anaesthetic used in the treatment of several disease related to ageing, asthma, and arthritis. In this study, we describe a rapid, sensitive, simple, and accurate method for the determination of PRC in milk, using an immunochromatographic test strip. For the immunochromatographic test, antigen (PRC-bovine serum albumin) and goat anti-mouse IgG were drawn onto a nitrocellulose membrane representing the control line and test line. The test was developed with a sensitive monoclonal antibody specific for PRC, generated by immunizing BALB/c mice with a well-characterized PRC- Keyhole limpet hemocyanin conjugate, produced in our laboratory. Under optimized conditions, the semi-quantitative test strip was able to detect PRC at concentrations down to 1 ng/mL in both 0.01 M phosphate buffer saline (pH 7.4) and milk samples. All results were obtained within 5 min. Our data indicate that the immunochromatographic assay is capable of sensitive, rapid, and specific on-site screening of PRC.
format	article
author	Kai Hao Steven Suryoprabowo Shanshan Song Liqiang Liu Qiankun Zheng Hua Kuang
author_facet	Kai Hao Steven Suryoprabowo Shanshan Song Liqiang Liu Qiankun Zheng Hua Kuang
author_sort	Kai Hao
title	Development of an immunochromatographic test strip for the detection of procaine in milk
title_short	Development of an immunochromatographic test strip for the detection of procaine in milk
title_full	Development of an immunochromatographic test strip for the detection of procaine in milk
title_fullStr	Development of an immunochromatographic test strip for the detection of procaine in milk
title_full_unstemmed	Development of an immunochromatographic test strip for the detection of procaine in milk
title_sort	development of an immunochromatographic test strip for the detection of procaine in milk
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/8831916419284c59ae4b6b197db87121
work_keys_str_mv	AT kaihao developmentofanimmunochromatographicteststripforthedetectionofprocaineinmilk AT stevensuryoprabowo developmentofanimmunochromatographicteststripforthedetectionofprocaineinmilk AT shanshansong developmentofanimmunochromatographicteststripforthedetectionofprocaineinmilk AT liqiangliu developmentofanimmunochromatographicteststripforthedetectionofprocaineinmilk AT qiankunzheng developmentofanimmunochromatographicteststripforthedetectionofprocaineinmilk AT huakuang developmentofanimmunochromatographicteststripforthedetectionofprocaineinmilk
_version_	1718409545835872256

Development of an immunochromatographic test strip for the detection of procaine in milk

Ejemplares similares